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101374 Brilliant green (hydrogen sulfate) (C.I. 42040)

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      Glass bottle 25 g
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      Catalogue Number101374
      SynonymsDiamond green G, Solid green, Ethyl green
      DescriptionBrilliant green (hydrogen sulfate) (C.I. 42040)
      OverviewBrilliant green (hydrogen sulfate) (C.I. 42040) for microscopy Certistain®, is a dry staining dye that is used to prepare a staining solution makes target structures in bacteriological specimen materials evaluable for diagnostic purposes. In the area of bacteriology, among others the Gram staining method, the Ziehl-Neelsen staining method, and the spore-staining method according to Rakette are used to yield a differentiated image of pathogens. This can be done either by using eosin Y as a counterstain or by modification according to Wirtz using safranine O solution. By this method, spores are always stained green and the cell elements always red.
      As an IVD product and CE registered, it can be used hassle-free in diagnostics and laboratory accreditations. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
      Product Information
      CAS number633-03-4
      EC number211-190-1
      Hill FormulaC₂₇H₃₄N₂O₄S
      Molar Mass482.65 g/mol
      HS Code3204 13 00
      Structure formula ImageStructure formula Image
      Quality LevelMQ400
      Applicationfor microscopy Certistain®
      Biological Information
      Physicochemical Information
      Melting Point210 °C (decomposition)
      pH value2.5 (10 g/l, H₂O, 25 °C)
      Bulk density500 - 600 kg/m3
      Solubility100 g/l
      Materials Information
      Toxicological Information
      LD 50 oralLD50 Rat 300 - 400 mg/kg
      Safety Information according to GHS
      Hazard Pictogram(s) Hazard Pictogram(s)
      Hazard Statement(s)H302: Harmful if swallowed.
      H319: Causes serious eye irritation.
      Precautionary Statement(s)P264: Wash skin thoroughly after handling.
      P270: Do not eat, drink or smoke when using this product.
      P280: Wear eye protection/ face protection.
      P301 + P312: IF SWALLOWED: Call a POISON CENTER/doctor if you feel unwell.
      P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
      P337 + P313: If eye irritation persists: Get medical advice/ attention.
      Signal WordWarning
      Storage class10 - 13 Other liquids and solids
      WGKWGK 3 highly hazardous to water
      Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.
      Safety Information
      Categories of dangerharmful, irritant
      Product Usage Statements
      Storage and Shipping Information
      StorageStore at +2°C to +30°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Dye content (spectrophotometrically)≥ 95 %
      - Hydrogen sulfate (as sulfur)

      passes test
      - UV/VIS-Spectrumpasses test
      Absorption maximum λ max (ethanol 50 %)628 - 632 nm
      Spec. Absorptivity A 1%/1cm (λmax; 0.003 g/l; ethanol 50 %)2210 - 2350
      TLC-Testpasses test
      Loss on drying (110 °C)≤ 5 %
      Suitability for microscopypasses test