ECM590 Blood Vessel Staining Kit

1 kit  sufficient for 100 sections
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      Key Specifications Table

      Species ReactivityKey ApplicationsDetection Methods
      H IHC, IH(P) Chromogenic
      Catalogue NumberECM590
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionBlood Vessel Staining Kit
      OverviewReagents and materials supplied in the Blood Vessel Staining Kit are sufficient for staining 100 tissue sections and when stored at 2-8ºC is stable up to the expiration date. ECM590 detects vWF and CD31 in human paraffin embedded tissues. In rat or mouse tissues only the vWF antibody is effective in paraffin; the CD31 antibody does not react with rodent CD31.


      Angiogenesis and tumor-associated neovascularization are complex processes by which new blood vessels are formed from pre-existing vasculature. Several studies implicate tumor-associated blood vessel formation as a central part in the process of growth, invasion and metastasis of malignancies (Rak et al., 1995; Jahroudi & Greenberger, 1995; Saphir, 1997).

      The most widely used endothelial cell markers for studying angiogenesis/neovascularization are von Willebrand Factor (Factor VIII Related Antigen) and CD31 (PECAM-1). von Willebrand Factor is synthesized by endothelial cells, causing adhesion of platelets to injured vessel walls and functions as a carrier and stabilizer for coagulation of Factor VIII. CHEMICON's von Willebrand Factor antibody reacts specifically with the endothelial cells of blood vessels and is a useful marker for the identification of endothelial lineage in tumors. It is important to note that not all endothelial cells synthesize von Willebrand factor, therefore a small percentage of these vascular-rich tumors might not stain with this antibody. CD31 or platelet endothelial adhesion molecule (PECAM-1), is a type I integral membrane glycoprotein and a member of the immunoglobulin super family of cell surface receptors. It is strongly expressed by all endothelial cells and weakly by several types of leucocytes (Parums et al., 1990). According to several studies, staining of blood vessels with CD31 antibodies has shown to be suitable for the identification of angiogenesis in several types of malignancies such as lung (Giatromanolaki et al., 1996), breast (Horak et al., 1992) and colorectal cancer (Takebayashi et al., 1996). CHEMICON's monoclonal antibody to CD31 is very specific for the staining of vascular endothelial cells in normal and cancerous tissue.

      CHEMICON's Blood Vessel Staining Kit is a very sensitive immunohistochemical tool for the detection of vascular endothelial cells, containing both anti-von Willebrand Factor and anti-CD31 primary antibodies and is applicable to a variety of species. This immunoperoxidase kit provides a simple, all-in-one method for the staining of frozen and paraffin-embedded sections in human (both CD31 and VWF) or rat and mouse (VWF only) tissues
      Materials Required but Not Delivered1. Tissue Sections/Slides: Paraffin-embedded or frozen sections can be used with this kit.

      2. 100% Ethanol.

      3. Xylene.

      4. Hematoxylin: If counterstaining if required.

      5. Solutions used for epitope/antigen retrieval: For example, 10 mM Sodium Citrate (pH 6.0) solution.

      6. Hydrogen Peroxide: Needed if quenching of endogenous peroxidase activity is required.

      7. Mounting Fluid: Aqueous or Xylene-based.

      8. Coverslips.

      9. Microscope.
      Product Information
      • Rabbit anti-vWF Polyclonal Antibody (Part No. 21540): One vial containing 100 μg at 1 mg/mL.
      • Mouse anti-CD31 Monoclonal Antibody (Part No. 90214): One vial containing 100 μL at 15 mg/mL (total protein including IgG and Carrier protein).
      • Goat anti-Rabbit Secondary Antibody (Part No. 21537): One 15 mL ready-to-use solution containing biotinylated goat anti-rabbit IgG in PBS with a carrier protein.
      • Goat anti-Mouse Secondary Antibody (Part No. 21538): One 15 mL ready-to-use solution containing biotinylated goat anti-mouse IgG in PBS with a carrier protein.
      • Blocking Reagent (Part No. 20783): One 15 mL ready-to-use solution containing normal goat serum in PBS with a carrier protein.
      • Streptavidin-HRP (Part No. 90215): One 15 ml ready-to-use solution of Streptavidin-HRP diluted in Tris-buffered saline (TBS).
      • DAB Chromogen A (Part No. 90216): One 5 mL bottle of 3, 3' Diaminobenzidine diluted in TBS.
      • DAB Chromogen B (Part No: 90217): Two 12 mL bottles of hydrogen peroxide diluted in distilled water.
      • 20X Rinse Buffer (Part No. 90218): One 275 mL bottle of 20X TBS.
      Detection methodChromogenic
      ApplicationReagents & materials supplied in the Blood Vessel Staining Kit are sufficient for staining 100 tissue sections & when stored at 2-8ºC is stable up to the expiration date.
      Key Applications
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      Application NotesECM590 detects vWF and CD31 in human paraffin embedded tissues. In rat or mouse tissues only the vWF antibody is effective in paraffin; the CD31 antibody does not react with rodent CD31.
      Biological Information
      Species Reactivity
      • Human
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsDo not freeze or expose to elevated temperatures. Discard any remaining reagents after the expiration date. Do not store kit components or perform staining in strong light, such as direct sunlight.
      Packaging Information
      Material Size1 kit
      Material Packagesufficient for 100 sections
      Transport Information
      Supplemental Information


      Blood Vessel Staining Kit SDS


      Safety Data Sheet (SDS) 


      Reference overviewPub Med ID
      Bioceramic-mediated trophic factor secretion by mesenchymal stem cells enhances in vitro endothelial cell persistence and in vivo angiogenesis.
      He, J; Decaris, ML; Leach, JK
      Tissue engineering. Part A  18  1520-8  2012

      Show Abstract
      22546052 22546052
      Brain-derived neurotrophic factor promotes tumorigenesis via induction of neovascularization: implication in hepatocellular carcinoma.
      Chi-Tat Lam,Zhen-Fan Yang,Chi-Keung Lau,Ka-Ho Tam,Sheung-Tat Fan,Ronnie T P Poon
      Clinical cancer research : an official journal of the American Association for Cancer Research  17  2011

      Show Abstract
      21421859 21421859
      Dopamine inhibits pulmonary edema through the VEGF-VEGFR2 axis in a murine model of acute lung injury.
      Vohra PK, Hoeppner LH, Sagar G, Dutta SK, Misra S, Hubmayr RD, Mukhopadhyay D
      American journal of physiology Lung cellular and molecular physiology  2011

      Show Abstract
      22003095 22003095
      Histidine-rich glycoprotein modulates the anti-angiogenic effects of vasculostatin.
      Klenotic PA, Huang P, Palomo J, Kaur B, Van Meir EG, Vogelbaum MA, Febbraio M, Gladson CL, Silverstein RL
      The American journal of pathology  176  2039-50 Epub 2010 Feb 18  2010

      Show Abstract Full Text Article
      20167858 20167858
      Structural variants of biodegradable polyesterurethane in vivo evoke a cellular and angiogenic response that is dictated by architecture.
      Jerome A Henry, Krishna Burugapalli, Peter Neuenschwander, Abhay Pandit
      Acta biomaterialia  5  29-42  2009

      Show Abstract
      18823827 18823827
      Angiogenesis: the unifying concept in cancer?
      Saphir, A
      J. Natl. Cancer Inst., 89: 1658-9 (1997)  1997

      9390530 9390530
      Prognostic value of angiogenesis in operable non-small cell lung cancer.
      Giatromanolaki, A, et al.
      J. Pathol., 179: 80-8 (1996)  1996

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      8691350 8691350
      Angiogenesis as an unfavorable prognostic factor in human colorectal carcinoma.
      Takebayashi, Y, et al.
      Cancer, 78: 226-31 (1996)  1996

      Show Abstract
      8673996 8673996
      Consequences of angiogenesis for tumor progression, metastasis and cancer therapy.
      Rak, J W, et al.
      Anticancer Drugs, 6: 3-18 (1995)  1995

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      7538829 7538829
      The role of endothelial cells in tumor invasion and metastasis.
      Jahroudi, N and Greenberger, J S
      J. Neurooncol., 23: 99-108 (1995)  1995

      Show Abstract
      7543941 7543941