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71546 BacMagic™ Transfection Kit - Novagen

71546
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      Description
      OverviewThe BacMagic™-1 Transfection Kit provides a positive selection for creating baculovirus recombinants in preparation for recombinant protein expression in insect cells. The kit improves on the traditional method for generating recombinant baculoviruses by eliminating the tedious, time-consuming steps of plaque purification. BacMagic DNA is an AcNPV genome with a portion of the essential open reading frame (ORF) 1629 deleted, preventing nonrecombinant virus from replicating in insect cells. The target coding sequence in a compatible transfer plasmid, such as pIEx/Bac™, pBAC™, or pTriEx™ vectors, is cotransfected with BacMagic DNA into insect cells. Homologous recombination within the cells restores the function of the viral ORF1629. Only recombinant baculovirus can replicate, producing a homogeneous population of recombinants. The BacMagic™-1 Transfection Kit contains the BacMagic™-1 DNA, Insect GeneJuice® Transfection Reagent, and a positive control plasmid to monitor co-transfection efficiency. In addition, it contains Sf9 cells and BacVector® Medium, for optimal transfection performance.
      Catalogue Number71546
      Brand Family Novagen®
      Application Data
      Figure 1. Construction of baculovirus recombinants with three BacMagic systems.

      Figure 2. Streamlined baculovirus method
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      Product Information
      0.5 µgBacMagic DNA
      50 µlInsect GeneJuice Transfection Reagent
      2 x 1 mlSf9 Cells
      1 LBacVector® Insect Cell Medium
      2 µgTransfection Control Plasmid
      Quality LevelMQ100
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      Ship Code Multiple Storage Conditions
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage Multiple storage requirements
      Do not freeze Ok to freeze
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