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2160 Autofluorescence Eliminator Reagent

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2160
50 mL  
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      Overview

      Replacement Information

      Key Specifications Table

      Key Applications
      IHC
      Description
      Catalogue Number2160
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAutofluorescence Eliminator Reagent
      OverviewReady-to-use for application in fluorescence microscopy procedures. With increasing age, the autofluorescent pigment lipofuscin accumulates in the cytoplasm of many cell types, including neurons. The presence of lipofuscin granules can complicate the use of fluorescence microscopy in the central nervous system because of its broad excitation and emission spectra, which overlaps with those of most commonly used fluorophores. The Autofluorescence Eliminator Reagent will reduce or eliminate lipofuscin-like autofluorescence without adversely affecting other fluorescent label in sections of human, monkey or rat neural tissue as well as other tissues.
      References
      Product Information
      PresentationLiquid in 70% ethanol containing no preservatives. Store at room temperature in the dark.
      Applications
      ApplicationAutofluorescence Eliminator Reagent for IHC.
      Key Applications
      • Immunohistochemistry
      Application NotesFluorescence immunohistochemistry counterstain.

      PROTOCOL:

      1.After immunofluorescence histochemistry, the sections are immersed in PBS for 5 min.·

      2.Sections are immersed in 70% ethanol for 5 min.·

      3.Sections are immersed in Autofluorescence Eliminator Reagent for 5 min.

      4.Sections are immersed in three changes of 70% ethanol for 1 min. each time.·

      5.Sections are mounted using an antifading solution (e.g. DABCO, non-xylene-based).



      Note: Reagent will block all fluorescence if incubation is too long; length of incubation time and number of 70% ethanol washes can be altered and optimized for various tissues and fluorescent antibody probes. In some instances the reagent can be applied prior to immunofluorescence, however in most cases better results are obtained when used as we direct above (use after immunofluorescence procedures are completed).



      Optimal time of incubation with the Autofluorescence Eliminator Reagent should be determined by the end user for each sample.

      Absorption maximum: 595-605
      Biological Information
      Physicochemical Information
      Dimensions
      Volume50 mL
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsWhen stored at room temperature, this reagent is stable up to the expiration date printed on the label. Do not freeze or expose to elevated temperatures.
      Packaging Information
      Material Size50 mL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Autofluorescence Eliminator Reagent SDS

      Title

      Safety Data Sheet (SDS) 

      Autofluorescence Eliminator Reagent Certificates of Analysis

      TitleLot Number
      AUTOFLUORESCENCE ELIMINATOR REAGENT 3147602
      AUTOFLUORESCENCE ELIMINATOR REAGENT 3156770
      AUTOFLUORESCENCE ELIMINATOR REAGENT 2987603
      AUTOFLUORESCENCE ELIMINATOR REAGENT 2952686
      AUTOFLUORESCENCE ELIMINATOR REAGENT 3016635
      AUTOFLUORESCENCE ELIMINATOR REAGENT 3040796
      AUTOFLUORESCENCE ELIMINATOR REAGENT 2840415
      AUTOFLUORESCENCE ELIMINATOR REAGENT 2861905
      AUTOFLUORESCENCE ELIMINATOR REAGENT 2905302
      AUTOFLUORESCENCE ELIMINATOR REAGENT 2899739

      References

      Reference overviewPub Med ID
      Deletion of the huntingtin proline-rich region does not significantly affect normal huntingtin function in mice.
      Neveklovska, M; Clabough, EB; Steffan, JS; Zeitlin, SO
      Journal of Huntington's disease  1  71-87  2012

      Show Abstract
      22956985 22956985

      Data Sheet

      Title
      AUTOFLUORESCENCE ELIMINATOR REAGENT