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07-449 Anti-trimethyl-Histone H3 (Lys27) Antibody

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07-449
200 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MICC, IP, Mplex, WB, IHCRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number07-449
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-trimethyl-Histone H3 (Lys27) Antibody
      Alternate Names
      • H3 histone family, member T
      • histone 3
      • H3
      • histone cluster 3
      • H3
      • H3k27me3
      Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
      References
      Product Information
      FormatPurified
      Control
      • HeLa whole cell lysate.
      PresentationPurified rabbit IgG in buffer containing 0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl with 0.05% sodium azide before the addition of 30% glycerol.
      Quality LevelMQ100
      Applications
      ApplicationAnti-trimethyl-Histone H3 (Lys27), also known as Anti-H3K27me3, is a highly published Rabbit Polyclonal Antibody. This protein A purified antibody is dot blot tested for trimethylated lysine 27 specificity and validated in WB, ICC, IP.
      Key Applications
      • Immunocytochemistry
      • Immunoprecipitation
      • Multiplexing
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunocytochemistry:

      Immunoprecipitation:

      Beadlyte® Histone-Peptide Specificity Assay:
      Biological Information
      ImmunogenKLH-conjugated, synthetic 2X-branched peptide containing the sequence …AR(me3K)SAP… in which me3K corresponds to trimethyl-lysine at residue 27 of human histone H3.
      EpitopeLys27
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityTrimethylated histone H3 (Lys27). Broad species cross-reactivity expected.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, mouse. Broad species cross-reactivity expected.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
      Gene Symbol
      • HIST1H3A
      • H3FA
      • HIST1H3B
      • H3FL
      • HIST1H3C
      • H3FC
      • HIST1H3D
      • H3FB
      • HIST1H3E
      • H3FD
      • HIST1H3F
      • H3FI
      • HIST1H3G
      • H3FH
      • HIST1H3H
      • H3FK
      • HIST1H3I
      • H3FF
      • HIST1H3J
      • H3FJ
      • HIST3H3
      • H3FT
      • H3T
      • MGC126886
      Modifications
      • Methylation
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 136 amino acids; 15508 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      SUBCELLULAR LOCATION: Nucleus.
      TISSUE SPECIFICITY: Expressed in testicular cells.
      DEVELOPMENTAL STAGE: Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
      PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity).
      SIMILARITY: SwissProt: Q16695 ## Belongs to the histone H3 family.
      Molecular Weight17 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by western blot in acid extracted proteins from HeLa cells.

      Western Blot Analysis:
      0.4 µg/mL of anti-trimethyl histone H3 Lys27 was used on recombinant Histone H3 and HeLa cell acid extracts.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-trimethyl-Histone H3 (Lys27) Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-trimethyl-Histone H3 (Lys27) Antibody Certificates of Analysis

      TitleLot Number
      Anti-Trimethyl-Histone H3 (Lys27) 2475696
      Anti-Trimethyl-Histone H3 (Lys27) - 2382150 2382150
      Anti-Trimethyl-Histone H3 (Lys27) - 2455635 2455635
      Anti-Trimethyl-Histone H3 (Lys27) - 2194165 2194165
      Anti-Trimethyl-Histone H3 (Lys27) - 2275589 2275589
      Anti-Trimethyl-Histone H3 (Lys27) - 2318778 2318778
      Anti-Trimethyl-Histone H3 (Lys27) - 2506493 2506493
      Anti-Trimethyl-Histone H3 (Lys27) - 3170806 3170806
      Anti-Trimethyl-Histone H3 (Lys27) - 3236354 3236354
      Anti-Trimethyl-Histone H3 (Lys27) - 3253330 3253330

      References

      Reference overviewApplicationSpeciesPub Med ID
      Bmi1 promotes erythroid development through regulating ribosome biogenesis.
      Gao, R; Chen, S; Kobayashi, M; Yu, H; Zhang, Y; Wan, Y; Young, SK; Soltis, A; Yu, M; Vemula, S; Fraenkel, E; Cantor, A; Antipin, Y; Xu, Y; Yoder, MC; Wek, RC; Ellis, SR; Kapur, R; Zhu, X; Liu, Y
      Stem Cells  33  925-38  2015

      Show Abstract
      25385494 25385494
      EZH2 modulates angiogenesis in vitro and in a mouse model of limb ischemia.
      Mitić, T; Caporali, A; Floris, I; Meloni, M; Marchetti, M; Urrutia, R; Angelini, GD; Emanueli, C
      Molecular therapy : the journal of the American Society of Gene Therapy  23  32-42  2015

      Show Abstract
      25189741 25189741
      TELP, a sensitive and versatile library construction method for next-generation sequencing.
      Peng, X; Wu, J; Brunmeir, R; Kim, SY; Zhang, Q; Ding, C; Han, W; Xie, W; Xu, F
      Nucleic acids research  43  e35  2015

      Show Abstract
      Mouse25223787 25223787
      Tissue-specific regulation of Igf2r/Airn imprinting during gastrulation.
      Marcho, C; Bevilacqua, A; Tremblay, KD; Mager, J
      Epigenetics & chromatin  8  10  2015

      Show Abstract
      25918552 25918552
      Regulation of p53 during senescence in normal human keratinocytes.
      Kim, RH; Kang, MK; Kim, T; Yang, P; Bae, S; Williams, DW; Phung, S; Shin, KH; Hong, C; Park, NH
      Aging cell  14  838-46  2015

      Show Abstract
      26138448 26138448
      Identification of in vivo DNA-binding mechanisms of Pax6 and reconstruction of Pax6-dependent gene regulatory networks during forebrain and lens development.
      Sun, J; Rockowitz, S; Xie, Q; Ashery-Padan, R; Zheng, D; Cvekl, A
      Nucleic acids research  43  6827-46  2015

      Show Abstract
      26138486 26138486
      Transcriptome and H3K27 tri-methylation profiling of Ezh2-deficient lung epithelium.
      Holik, AZ; Galvis, LA; Lun, AT; Ritchie, ME; Asselin-Labat, ML
      Genomics data  5  346-51  2015

      Show Abstract
      26484284 26484284
      Cytomixis doesn't induce obvious changes in chromatin modifications and programmed cell death in tobacco male meiocytes.
      Mursalimov, S; Permyakova, N; Deineko, E; Houben, A; Demidov, D
      Frontiers in plant science  6  846  2015

      Show Abstract
      26528310 26528310
      Global analysis of H3K4me3 and H3K27me3 profiles in glioblastoma stem cells and identification of SLC17A7 as a bivalent tumor suppressor gene.
      Lin, B; Lee, H; Yoon, JG; Madan, A; Wayner, E; Tonning, S; Hothi, P; Schroeder, B; Ulasov, I; Foltz, G; Hood, L; Cobbs, C
      Oncotarget  6  5369-81  2015

      Show Abstract
      25749033 25749033
      Epigenetic silencing of Oct4 by a complex containing SUV39H1 and Oct4 pseudogene lncRNA.
      Scarola, M; Comisso, E; Pascolo, R; Chiaradia, R; Maria Marion, R; Schneider, C; Blasco, MA; Schoeftner, S; Benetti, R
      Nature communications  6  7631  2015

      Show Abstract
      26158551 26158551

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      Catalog Number Description  
      ABE44 Anti-trimethyl Histone H3 (Lys27) Antibody Show Pricing & Availability

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      Life Science Research > Antibodies and Assays > Primary Antibodies