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07-449-AF555 Anti-trimethyl Histone H3 (Lys27), Alexa Fluor® 555 Conjugate Antibody

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07-449-AF555
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICCRbAlexaFluor®555Polyclonal Antibody
      Description
      Catalogue Number07-449-AF555
      DescriptionAnti-trimethyl Histone H3 (Lys27), Alexa Fluor® 555 Conjugate Antibody
      Alternate Names
      • Histone H3.1t
      • H3/t
      • H3t
      • H3/g
      Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Histone proteins are highly post-translationally modified however Histone H3 is the most extensively modified of the five histones. Histone H3 sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes. Trimethylation of histone H3 on Lys9 (H3K9me3) is one of the most highly studied epigenetic marks. H3K9me3 functions in the repression of euchromatic genes, and in epigenetic control of heterochromatin assembly, most likely by acting as a recognition motif for the binding of chromatin-associated proteins, such as Swi6 or HP1α. The enzymes responsible for H3K9 trimethylation are SUV39H1 and SUV39H2.
      References
      Product Information
      FormatAlexaFluor®555
      PresentationPurified rabbit IgG in PBS containing 15 mg/mL BSA and 0.1% Sodium Azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-trimethyl Histone H3 (Lys27) is validated for use in Immunocytochemistry for the detection of trimethyl Histone H3 (Lys27).
      Key Applications
      • Immunocytochemistry
      Biological Information
      ImmunogenBranch peptide corresponding to trimethyl-Histone H3 (Lys27).
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThe unconjugated parent antibody of this antibody is Cat. No. 07-449.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Mouse based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HIST3H3
      • H3FT
      Purification MethodProtein A Purfied
      UniProt Number
      Molecular Weight~17 kDa observed. The unconjugated parent antibody (Cat. No. 07-449) has an observed molecular weight at ~17 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells.

      Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected trimethyl-Histone H3 (Lys27) in HeLa cells.
      Alexa Fluor® is a registered trademark of Life Technologies®.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-trimethyl Histone H3 (Lys27), Alexa Fluor® 555 Conjugate Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-trimethyl Histone H3 (Lys27), Alexa Fluor® 555 Conjugate Antibody Certificates of Analysis

      TitleLot Number
      Anti-trimethyl Histone H3 (Lys27), -2569696 2569696
      Anti-trimethyl Histone H3 (Lys27), -2569810 2569810
      Anti-trimethyl Histone H3 (Lys27), Alexa Fluor® 555 Conjugate 3050588
      Anti-trimethyl Histone H3 (Lys27), Alexa Fluor® 555 Conjugate - Q2443133 Q2443133
      Anti-trimethyl Histone H3 (Lys27), Alexa Fluor® 555 Conjugate - 3714301 3714301

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies