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ABS1631 Anti-phospho-eNOS (Ser602)

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ABS1631
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      BWB, ELISARbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberABS1631
      DescriptionAnti-phospho-eNOS (Ser602)
      Alternate Names
      • Nitric oxide synthase endothelial
      • Constitutive NOS
      • EC-NOS
      • eNOS
      • NOS III
      • NOS type III
      Background InformationNitric oxide synthase, endothelial (UniProt: P29473; also known as EC:1.14.13.39; constitutive NOS, EC-NOS, eNOS, NOS III, NOS type III) is encoded by the NOS3 gene (Gene ID: 287024) in bovine. The eNOS is mainly expressed in vascular endothelial cells and cardiomyocytes. It produces nitric oxide (NO) that is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. In endothelial cells, eNOS is localized in specialized plasmalemmal pockets known as caveolae, which contain a scaffolding protein termed caveolin. Acylation of eNOS by either myristate or palmitate targets it to caveolae, which facilitates enzyme activation following receptor stimulation. In a resting cell, eNOS is inhibited by its interaction with caveolin. Following agonist activation, eNOS dissociates from caveolin and translocates to the cytosol. The eNOS is generally activated in response to an increase in intracellular Ca2+ following stimulation with receptor-dependent agonists such as acetylcholine. However, shear stress can also activate this enzyme independently of Ca2+ level changes, via a tyrosine kinase-dependent mechanism involving its phosphorylation and redistribution. Perturbations of eNOS phosphorylation have been reported in a number of diseases. The primary sites where eNOS gets phosphorylated are multiple serine residues and, to a lesser extent, on tyrosine and threonine residues. Ser 602 is reported to lie at the N-terminal edge of the 42-residue eNOS autoinhibitory element and is phosphorylated by ERK. Low concentrations of nitric oxide in the endothelial cells prevent their apoptotic death and maintain their integrity during inflammation and atherosclerosis. Distorted production of nitric oxide in endothelial cells has been linked to hypertension, hypercholesterolemia, diabetes, and even cardiac failure. (Ref.: Rafikov, R., et al. (2011). J. Endocrinol. 210(3): 271-284).
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in PBS with 0.02% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect endothelial Nitric oxide synthase using this rabbit polyclonal Anti-phospho-eNOS (Ser602), Cat. No. ABS1631. It has been tested for use in ELISA and Western Blotting.
      Key Applications
      • Western Blotting
      • ELISA
      Application NotesWestern Blotting Analysis: A representative lot detected phospho-eNOS (Ser602) in bovine aortic endothelial cell lysate (BAEC) (Salerno, J.C. et. al. (2014). Biosci Rep. 34(5). pii: e00137).

      Western Blotting Analysis: A 1:250-1,000 dilution from a representative lot detected phospho-eNOS (Ser602) in bovine aortic endothelial cell lysates (Courtesy of Dr. Carol Chrestensen at Kennesaw State University, Kennesaw, GA).

      ELISA Analysis: A 1:64,000 dilution from a representative lot detected phospho-eNOS (Ser602) in Phospho-602 peptide/Non-Phospho-602 peptide (Courtesy of Dr. Carol Chrestensen at Kennesaw State University, Kennesaw, GA).


      Biological Information
      ImmunogenA linear peptide of 11 amino acids from the N-terminal edge of the 42-residue bovine eNOS autoinhibitory element.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis polyclonal antibody specifically detects the phospho-Ser602 in eNOS. It targets a sequence of 11 amino acids surrounding Ser602.
      Species Reactivity
      • Bovine
      Species Reactivity NoteBovine. Predicted to react with Human based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NOS3
      Modifications
      • Phosphorylation
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~150 kDa observed; 133.29 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in bovine aortic endothelial cell lysates.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected phospho-eNOS (Ser602) in 10 µg of bovine aortic endothelial cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications