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07-428 Anti-phospho-eNOS (Ser1177) Antibody

07-428
200 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, B, CaWBRbSerumPolyclonal Antibody
      Description
      Catalogue Number07-428
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-eNOS (Ser1177) Antibody
      OverviewNote: ADMA (Catalog #19-169) and NMMA (Catalog #19-170) are inhibitors of NOS
      Alternate Names
      • Constitutive NOS
      • Endothelial NOS
      • Endothelial NOS
      • Endothelial NOS
      • endothelial nitric oxide synthase
      • nitric oxide synthase 3 (endothelial cell)
      Background InformationNO, produced by nitric oxide synthases (NOS), is a critical signaling molecule involved in many physiological processes including muscle contraction, vasodilation, neuronal transmission, and immune responses. Some of these effects are mediated through reaction with and activation of guanylate cyclase (GC), resulting in increased cGMP production. With a half-life of seconds, NO readily diffuses through the cytosol and cell membrane, allowing it to act inside or adjacent to the cell in which it was produced.
      References
      Product Information
      FormatSerum
      Control
      • Brain tissue, VEGF treated HUVEC cell lysate.
      PresentationRabbit polyclonal antiserum IgG in buffer containing 0.05% sodium azide and glycerol to 30%. Liquid at -20°C.
      Applications
      ApplicationAnti-phospho-eNOS (Ser1177) Antibody detects level of phospho-eNOS (Ser1177) & has been published & validated for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide (C-RIRTQ[pS]FSLQER) corresponding to amino acids 1172-1183 of human eNOS (endothelial nitric oxide synthase) with an N-terminal cysteine added for conjugation purposes. The immunizing sequence is identical in rat, mouse, bovine, pig, and dog.
      EpitopeSer1177
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes phosphorylated eNOS, Mr 132 kDa. Additional unknown bands at 50 and 64 kDa were competed by the immunizing peptide.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Bovine
      • Canine
      Species Reactivity NoteHuman; other species not tested. Predicted to cross-react with mouse, rat, cow, and dog based on sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NOS3
      • cNOS
      • ECNOS
      • eNOS
      • EC-NOS
      • NOSIII
      Modifications
      • Phosphorylation
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P29474 # Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. No mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
      COFACTOR: Heme group. & Binds 1 FAD. & Binds 1 FMN. & Metrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.
      SIZE:1203 amino acids; 133289 Da
      SUBUNIT: Homodimer. Interacts with NOSIP and NOSTRIN.
      SUBCELLULAR LOCATION:Cell membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Note=Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity.
      TISSUE SPECIFICITY: Platelets, placenta, liver and kidney.
      SIMILARITY: SwissProt: P29474 ## Belongs to the NOS family. & Contains 1 flavodoxin-like domain.
      Molecular Weight132 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by western blot from VEGF treated HUVEC cell lysates.

      Western Blot Analysis:
      A 1:1000 dilution of this lot detected recombinant eNOS phosphorylated by PKA and endogenous eNOS from VEGF treated HUVEC cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      For maximum recovery of product, centrifuge the vial prior to removing the cap.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-phospho-eNOS (Ser1177) Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-phospho-eNOS (Ser1177) Antibody Certificates of Analysis

      TitleLot Number
      Anti-phospho-eNOS (Ser1177) (rabbit antiserum) - DAM1394809 DAM1394809
      Anti-phospho-eNOS (Ser1177) - 1972309 1972309
      Anti-phospho-eNOS (Ser1177) - 1992442 1992442
      Anti-phospho-eNOS (Ser1177) - 2068184 2068184
      Anti-phospho-eNOS (Ser1177) - DAM1479606 DAM1479606
      Anti-phospho-eNOS (Ser1177) - DAM1713221 DAM1713221
      Anti-phospho-eNOS (Ser1177) - DAM1745332 DAM1745332
      Anti-phospho-eNOS (Ser1177) - DAM1772424 DAM1772424
      Anti-phospho-eNOS (Ser1177) - DAM1821142 DAM1821142
      Anti-phospho-eNOS (Ser1177) - JBC1872197 JBC1872197

      References

      Reference overviewApplicationSpeciesPub Med ID
      Calcimimetic R-568 and its enantiomer S-568 increase nitric oxide release in human endothelial cells.
      Bonomini, M; Giardinelli, A; Morabito, C; Di Silvestre, S; Di Cesare, M; Di Pietro, N; Sirolli, V; Formoso, G; Amoroso, L; Mariggiò, MA; Pandolfi, A
      PloS one  7  e30682  2012

      Show Abstract
      Western BlottingHuman22295103 22295103
      Src kinase activates endothelial nitric-oxide synthase by phosphorylating Tyr-83.
      Fulton, D; Church, JE; Ruan, L; Li, C; Sood, SG; Kemp, BE; Jennings, IG; Venema, RC
      The Journal of biological chemistry  280  35943-52  2005

      Show Abstract
      16123043 16123043
      Acute activation and phosphorylation of endothelial nitric oxide synthase by HMG-CoA reductase inhibitors.
      Harris, MB; Blackstone, MA; Sood, SG; Li, C; Goolsby, JM; Venema, VJ; Kemp, BE; Venema, RC
      American journal of physiology. Heart and circulatory physiology  287  H560-6  2004

      Show Abstract
      15087285 15087285
      The Akt kinase signals directly to endothelial nitric oxide synthase.
      Michell, B J, et al.
      Curr. Biol., 9: 845-8 (1999)  1999

      Show Abstract
      10469573 10469573
      AMP-activated protein kinase phosphorylation of endothelial NO synthase.
      Chen, Z P, et al.
      FEBS Lett., 443: 285-9 (1999)  1999

      Show Abstract
      10025949 10025949

      Brochure

      Title
      Pathways and Biomarkers of Oxidative Stress (EMD)

      Technical Info

      Title
      White Paper- Modern Methods in Oxidative Stress Research (EMD)

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies