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04-811 Anti-phospho-eNOS/NOS III (Thr495) Antibody, rabbit monoclonal

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      Replacement Information
      Replacement InformationCat. # 05-811. 05-811 will only be availabe on a custom basis.

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      BWBRbCulture SupernatantMonoclonal Antibody
      Catalogue Number04-811
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-eNOS/NOS III (Thr495) Antibody, rabbit monoclonal
      OverviewNote: ADMA (Catalog #19-169) and NMMA (Catalog #19-170) are inhibitors of NOS
      Alternate Names
      • NOS III
      • endothelial nitric oxidase synthase
      • endothelial nitric oxide synthase
      • nitric oxide synthase 3 (endothelial cell)
      Product Information
      FormatCulture Supernatant
      • Fetal bovine heart endothelial cell lysate
      PresentationCultured supernatant in 0.05% sodium azide
      Quality LevelMQ100
      ApplicationThis Anti-phospho-eNOS/NOS III (Thr495) Antibody is validated for use in WB for the detection of phospho-eNOS/NOS III (Thr495).
      Key Applications
      • Western Blotting
      Application NotesWestern Blot Analysis:
      A 1:2,000-1:10,000 dilution of this lot detected phosphorylated eNOS/NOS III in fetal bovine heart endothelial cell lysate.
      Biological Information
      ImmunogenPeptide containing the sequence KK[pT]FK in which pT corresponds to phosphothreonine at residue 495 of human eNOS/NOS III
      SpecificityRecognizes phosphorylated eNOS/NOS III.
      Species Reactivity
      • Bovine
      Species Reactivity NotePredicted cross-reactivity with human, rabbit, mouse and rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NOS3
      • cNOS
      • ECNOS
      • eNOS
      • EC-NOS
      • NOSIII
      • Phosphorylation
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P29474 # Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. No mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
      COFACTOR: Heme group. & Binds 1 FAD. & Binds 1 FMN. & Metrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.
      SIZE: 1203 amino acids; 133289 Da
      SUBUNIT: Homodimer. Interacts with NOSIP and NOSTRIN.
      SUBCELLULAR LOCATION: Cell membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Note=Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity.
      TISSUE SPECIFICITY: Platelets, placenta, liver and kidney.
      SIMILARITY: SwissProt: P29474 ## Belongs to the NOS family. & Contains 1 flavodoxin-like domain.
      Molecular Weight135 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on fetal bovine heart endothelial cell lysate
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information


      Anti-phospho-eNOS/NOS III (Thr495) Antibody, rabbit monoclonal SDS


      Safety Data Sheet (SDS) 

      Anti-phospho-eNOS/NOS III (Thr495) Antibody, rabbit monoclonal Certificates of Analysis

      TitleLot Number
      Anti-phospho-eNOS/NOS III (Thr495) 3172126
      Anti-phospho-eNOS/NOS III (Thr495) 2840424
      Anti-phospho-eNOS/NOS III (Thr495) - 2137052 2137052
      Anti-phospho-eNOS/NOS III (Thr495) - 2492799 2492799
      Anti-phospho-eNOS/NOS III (Thr495) - 3003697 3003697
      Anti-phospho-eNOS/NOS III (Thr495) - DAM1495797 DAM1495797
      Anti-phospho-eNOS/NOS III (Thr495) - JBC1482108 JBC1482108
      Anti-phospho-eNOS/NOS III (Thr495) - NG1873186 NG1873186
      Anti-phospho-eNOS/NOS III (Thr495) -2743612 2743612
      Anti-phospho-eNOS/NOS III (Thr495) -2769017 2769017


      Reference overviewPub Med ID
      Novel protective role of endogenous cardiac myocyte P2X4 receptors in heart failure.
      Yang, T; Shen, JB; Yang, R; Redden, J; Dodge-Kafka, K; Grady, J; Jacobson, KA; Liang, BT
      Circulation. Heart failure  7  510-8  2014

      Show Abstract
      24622244 24622244


      Pathways and Biomarkers of Oxidative Stress (EMD)

      Technical Info

      White Paper- Modern Methods in Oxidative Stress Research (EMD)

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      Life Science Research > Antibodies and Assays > Primary Antibodies