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07-357 Anti-phospho-eNOS/NOS III (Ser114) Antibody

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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, HWBRbAffinity PurifiedPolyclonal Antibody
      Catalogue Number07-357
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-eNOS/NOS III (Ser114) Antibody
      OverviewNote: ADMA (Catalog #19-169) and NMMA (Catalog #19-170) are inhibitors of NOS
      Product Information
      FormatAffinity Purified
      Presentation0.2 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.1 mM EDTA, and 0.05% sodium azide before the addition of glycerol to 30%
      Quality LevelMQ100
      ApplicationDetect phospho-eNOS/NOS III (Ser114) using this Anti-phospho-eNOS/NOS III (Ser114) Antibody validated for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenSynthetic peptide corresponding to amino acids 106-118 of human eNOS/NOS III (Ser114) (PRKLQGRPpSPGPP-C) with a C-terminal cysteine added for conjugation to KLH. The immunizing sequence shares 12/13 amino acids with pig and bovine eNOS/NOS III.
      SpecificityeNOS/NOS III phosphorylated on serine 114
      Species Reactivity
      • Bovine
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NOS3
      • cNOS
      • ECNOS
      • eNOS
      • EC-NOS
      • NOSIII
      • Phosphorylation
      Purification MethodImmunoaffinity Chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P29474 # Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. No mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
      COFACTOR: Heme group. & Binds 1 FAD. & Binds 1 FMN. & Metrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.
      SIZE: 1203 amino acids; 133289 Da
      SUBUNIT: Homodimer. Interacts with NOSIP and NOSTRIN.
      SUBCELLULAR LOCATION: Cell membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Note=Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity.
      TISSUE SPECIFICITY: Platelets, placenta, liver and kidney.
      SIMILARITY: SwissProt: P29474 ## Belongs to the NOS family. & Contains 1 flavodoxin-like domain.
      Molecular Weight132 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot in fetal bovine heart endothelial cell lysate
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions1 year at -20°C
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information


      Anti-phospho-eNOS/NOS III (Ser114) Antibody SDS


      Safety Data Sheet (SDS) 

      Anti-phospho-eNOS/NOS III (Ser114) Antibody Certificates of Analysis

      TitleLot Number
      Anti-phospho-eNOS/NOS III (Ser114) (rabbit immunoaffinity purified IgG) 2973090
      Anti-phospho-eNOS/NOS III (Ser114) - 3469483 3469483
      Anti-phospho-eNOS/NOS III (Ser114) -2787698A 2787698A
      Anti-phospho-eNOS/NOS III (Ser114) -2852433 2852433
      Anti-phospho-eNOS/NOS III (Ser116) (rabbit immunoaffinity purified IgG) - 2149489 2149489
      Anti-phospho-eNOS/NOS III (Ser116) (rabbit immunoaffinity purified IgG) - 2424503 2424503
      Anti-phospho-eNOS/NOS III (Ser116) (rabbit immunoaffinity purified IgG) - 2273974 2273974
      Anti-phospho-eNOS/NOS III (Ser116) - 22407 22407
      Anti-phospho-eNOS/NOS III (Ser116) - 28830 28830
      Anti-phospho-eNOS/NOS III (Ser116) -2604783 2604783


      Reference overviewPub Med ID
      Functional protein network activation mapping reveals new potential molecular drug targets for poor prognosis pediatric BCP-ALL.
      Accordi, Benedetta, et al.
      PLoS ONE, 5: e13552 (2010)  2010

      Show Abstract
      21042412 21042412


      Pathways and Biomarkers of Oxidative Stress (EMD)

      Technical Info

      White Paper- Modern Methods in Oxidative Stress Research (EMD)

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      Life Science Research > Antibodies and Assays > Primary Antibodies