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07-1294 Anti-phospho-VEGFR2/Flk-1/KDR (Tyr1223) Antibody

07-1294
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, R, Pm, Ca, B, Po, EqWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-1294
      Trade Name
      • Upstate
      DescriptionAnti-phospho-VEGFR2/Flk-1/KDR (Tyr1223) Antibody
      Alternate Names
      • Kinase insert domain receptor
      • Protein-tyrosine kinase receptor Flk-1
      • kinase insert domain receptor (a type III receptor tyrosine kinase)
      • vascular endothelial growth factor receptor 2
      Background InformationThe Flt gene family of receptor tyrosine kinases includes Flt-1, KDR/Flk-1, Flt-3, and Flt-4. They encode receptors for VEGF, characterized by seven Ig-loops within their extracellular domain and a split kinase domain within the cytoplasmic domain. VEGF is an important angiogenic factor of many solid tumors and is also involved in the differentiation of endothelial cells. Flt-1 (Fms-like tyrosine kinase, VEGF-R1) is expressed on endothelial cells and on the common precursors of endothelial and hematopoietic stem cells. KDR (Flk-1, VEGF-R2) is the principal receptor involved in VEGF signaling. KDR is expressed on endothelial cells and stimulates angiogenesis, but is also expressed on bone marrow cells, and is thought to play a role in driving the proliferation of most or all leukemic cells. Flt3 is a class III receptor tyrosine kinase that is closely related to c-Kit and c-Fms. It is expressed in primitive hematopoietic cells and its ligand exerts proliferative effects on these cells in co-operation with other cytokines. The VEGF receptors stimulate the Ras-MAP kinase pathway, suggesting that it signals as a conventional receptor tyrosine kinase.
      References
      Product Information
      FormatAffinity Purified
      Control
      • CSF-1 treated PAE cell lysate, transfected with CSF/KDR.
      PresentationDouble affinity purified rabbit polyclonal in PBS with 0.05% NaN3 and 50% glycerol.
      Applications
      ApplicationThis Anti-phospho-VEGFR2/Flk-1/KDR (Tyr1223) Antibody is validated for use in WB for the detection of phospho-VEGFR2/Flk-1/KDR (Tyr1223).
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenSynthetic peptide encompassing phosphorylated Tyr1223 of human VEGFR2.
      HostRabbit
      SpecificityRecognizes VEGFR2/Flk-1/KDR when phosphorylated on Tyrosine 1223 (human).
      Species Reactivity
      • Mouse
      • Rat
      • Primate
      • Canine
      • Bovine
      • Pig
      • Horse
      Species Reactivity NoteHuman tested. Expected to react with Mouse, Rat, Primate, Canine, Cow, Pig, Horse, based on sequence homolgy.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • KDR
      • VEGFR-2
      • VEGFR2
      • VEGFR
      • CD309
      • FLK1
      • EC 2.7.10.1
      Modifications
      • Phosphorylation
      Purification MethodDual Antigen Affinity purification
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P35968 # Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
      SIZE: 1356 amino acids; 151527 Da
      SUBUNIT: Interacts with SHB upon VEGF activation. Interacts with HIV-1 Tat.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      SIMILARITY: SwissProt: P35968 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. & Contains 7 Ig-like C2-type (immunoglobulin-like) domains. & Contains 1 protein kinase domain. ...
      Molecular Weight152 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceWestern Blot Analysis:
      This lot detected VEGFR2 phosphorylated on Tyr1223 at a 1:1,000 dilution in PAE cells transfected with CSF/KDR and treated with 40ng/mL CSF-1 for 15 min. Cell lysate resolved via SDS-PAGE and transferred to PVDF (Immobilon-P).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies