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ABF131 Anti-phospho TIE2/TEK Antibody (Tyr992)

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ABF131
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IP, PIARbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABF131
      Replaces09-407
      DescriptionAnti-phospho TIE2/TEK Antibody (Tyr992)
      Alternate Names
      • Angiopoietin-1 receptor
      • Endothelial tyrosine kinase
      • Tunica interna endothelial cell kinase
      • Tyrosine kinase with Ig and EGF homology domains-2
      • Tyrosine-protein kinase receptor TEK
      • Tyrosine-protein kinase receptor TIE-2
      • hTIE2
      • p140 TEK
      • CD202b
      Background InformationTIE2/TEK (tyrosine kinase with Ig and EGF homology domains 2) is expressed almost exclusively in endothelial cells in mice, rats and humans. This receptor possesses a unique extracellular domain containing two immunoglobulin-like loops separated by three epidermal growth factor-like repeats that are connected to three fibronectin type III-like repeats. The ligand for the receptor is Angiopoietin 1. Defects in TIE2/TEK are associated with inherited venous malformations; the TIE2/TEK signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-phospho TIE2/TEK Antibody (Tyr992) is validated for use in western blotting, IP & peptide inhibition assay for the detection of phospho TIE2/TEK (Tyr992).
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Peptide Inhibition Assay
      Application NotesImmunoprecipitation Analysis: 5 µL from a representative lot immunoprecipitated phospho TIE2/TEK (Tyr992) from Pervanadate treated HUVEC cell lysate. Immunoprecipated sample was then subjected to Western Blotting using a 1:5,000 dilution of this antibody.

      Peptide Inhibition Assay Analysis: A 1:500 dilution from a representative lot detected phospho TIE2/TEK (Tyr992) from transfected HEK293T lysates when pre-incubated without or with non-phospho peptide, and not when pre-incubated with phospho peptide.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the Protein Kinase Domain of human TIE2/TEK phosphorylated at Tyr992.
      EpitopeProtein Kinase Domain
      Concentration0.25 mg/mL
      HostRabbit
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Mouse, Rat, Bovine, and Zebrafish based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TEK
      • TIE2
      • VMCM
      • VMCM1
      Modifications
      • Phosphorylation
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~125 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in untreated and Pervanadate treated HUVEC cell lysate.

      Western Blotting Analysis: A 1:5,000 dilution of this antibody detected phospho TIE2/TEK (Tyr992) in 10 µg of Pervanadate treated HUVEC cell lysate, and not in untreated HUVEC cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-phospho TIE2/TEK Antibody (Tyr992) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-phospho TIE2/TEK Antibody (Tyr992) Certificates of Analysis

      TitleLot Number
      Anti-phospho TIE2/TEK (Tyr992) 3073761
      Anti-phospho TIE2/TEK (Tyr992) - Q2420919 Q2420919
      Anti-phospho TIE2/TEK (Tyr992) - 3434784 3434784
      Anti-phospho TIE2/TEK (Tyr992) - 3517907 3517907
      Anti-phospho TIE2/TEK (Tyr992) - 3758615 3758615
      Anti-phospho TIE2/TEK (Tyr992) -2683262 2683262
      Anti-phospho TIE2/TEK (Tyr992) -2788125 2788125
      Anti-phospho TIE2/TEK (Tyr992) Polyclonal Antibody 2887486
      Anti-phospho TIE2/TEK (Tyr992) Polyclonal Antibody 2953822
      Anti-phospho TIE2/TEK (Tyr992) Polyclonal Antibody 2865446

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies