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07-175 Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) Antibody

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07-175
200 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, H, R, Ca, Xn, Ch, B, EqWB, Enzyme Assays, IPRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-175
      DescriptionAnti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) Antibody
      Alternate Names
      • JNK1 alpha protein kinase
      • JNK1 beta protein kinase
      • JUN N-terminal kinase
      • Stress-activated protein kinase JNK1
      • Stress-activated protein kinase JNK1
      • mitogen-activated protein kinase 8
      • mitogen-activated protein kinase 8 isoform JNK1 alpha1
      • mitogen-activated protein kinase 8 isoform JNK1 alpha1
      • protein kinase JNK1
      Background InformationJNK1(MAPK8) is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-α) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrome c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. JNK1 is activated by threonine and tyrosine phosphorylation by either of two dual specificity kinases, MAP2K4 and MAP2K7. The JNK pathway is critically involved in diabetes and levels are abnormally elevated in obesity. The cell-permeable JNK inhibitory peptide may have promise as a therapeutic agent for diabetes.
      References
      Product Information
      FormatAffinity Purified
      HS Code3002 15 90
      Control
      • Untreated and Lambda phosphatase treated RAW264.7 cell lysates
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect phospho-JNK (Thr183/Tyr185 using this Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) Antibody validated for use in WB, EA & IP.
      Key Applications
      • Western Blotting
      • Enzyme Assays
      • Immunoprecipitation
      Application NotesImmunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated JNK phosphorylated at Thr183/Tyr185 and Thr221/Tyr223 from RAW264.7 cell lysate.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human JNK phosphorylated at Thr183/Tyr185 and Thr221/Tyr223.
      EpitopePhosphorylated at Thr183/Tyr185 and Thr221/Tyr223
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes JNK when phosphorylated at Thr183/Tyr185 and Thr221/Tyr223 .
      IsotypeIgG
      Species Reactivity
      • Mouse
      • Human
      • Rat
      • Canine
      • Xenopus
      • Chicken
      • Bovine
      • Horse
      Species Reactivity NoteDemonstrated to react with mouse and human. Predicted to react with rat, canine, xenopus, chicken, bovine, and equine based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.
      Gene Symbol
      • MAPK8
      • JNK1
      • JNK21B1/2
      • PRKM8
      • JNK-46
      • JNK
      • SAPK1
      • JNK1A2
      Modifications
      • Phosphorylation
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta- 2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.

      COFACTOR: Magnesium.

      SIZE: 427 amino acids; 48296 Da

      SUBUNIT: Binds to at least four scaffolding proteins, MAPK8IP1/JIP-1, MAPK8IP2/JIP-2, MAPK8IP3/JIP-3/JSAP1 and SPAG9/MAPK8IP4/JIP-4. These proteins also bind other components of the JNK signaling pathway. Interacts with TP53 and WWOX. Interacts with JAMP (By similarity).

      DOMAIN: The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

      PTM: Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.

      SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain. ... hide » see more »
      Molecular Weight~ 42 kDa observed. There are several isoforms between 40-50 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in untreated and lambda phosphatase treated RAW264.7 cell lysates.

      Western Blot Analysis: 2 µg/mL of this antibody detected JNK on 10 µg of untreated and lambda phosphatase RAW264.7 cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      07-175 08436037124822

      Documentation

      Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) Antibody Certificates of Analysis

      TitleLot Number
      Anti-phospho-JNK (Thr183/Tyr185, -2664268 2664268
      Anti-phospho-JNK (Thr183/Tyr185, -2817871 2817871
      Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) - 2430491 2430491
      Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) - DAM1713218 DAM1713218
      Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) - DAM1810820 DAM1810820
      Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) - 20316 20316
      Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) - 2073119 2073119
      Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) - 2276339 2276339
      Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) - 2318094 2318094
      Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) - 2517878 2517878

      References

      Reference overviewApplicationPub Med ID
      Acylcarnitines activate proinflammatory signaling pathways.
      Rutkowsky, JM; Knotts, TA; Ono-Moore, KD; McCoin, CS; Huang, S; Schneider, D; Singh, S; Adams, SH; Hwang, DH
      American journal of physiology. Endocrinology and metabolism  306  E1378-87  2014

      Show Abstract
      24760988 24760988
      Cardiotoxin III inhibits proliferation and migration of oral cancer cells through MAPK and MMP signaling.
      Yen, CY; Liang, SS; Han, LY; Chou, HL; Chou, CK; Lin, SR; Chiu, CC
      TheScientificWorldJournal  2013  650946  2013

      Show Abstract
      Western Blotting23710144 23710144
      Upon Wnt stimulation, Rac1 activation requires Rac1 and Vav2 binding to p120-catenin.
      Valls, G; Codina, M; Miller, RK; Del Valle-Pérez, B; Vinyoles, M; Caelles, C; McCrea, PD; García de Herreros, A; Duñach, M
      Journal of cell science  125  5288-301  2012

      Show Abstract
      22946057 22946057
      Activation of c-Jun N-terminal kinase (JNK) during mitosis in retinal progenitor cells.
      Ribas, VT; Gonçalves, BS; Linden, R; Chiarini, LB
      PloS one  7  e34483  2012

      Show Abstract
      22496813 22496813
      CD22 regulates adaptive and innate immune responses of B cells.
      Kawasaki, N; Rademacher, C; Paulson, JC
      Journal of innate immunity  3  411-9  2011

      Show Abstract
      21178327 21178327
      MEK4 function, genistein treatment, and invasion of human prostate cancer cells.
      Xu, L; Ding, Y; Catalona, WJ; Yang, XJ; Anderson, WF; Jovanovic, B; Wellman, K; Killmer, J; Huang, X; Scheidt, KA; Montgomery, RB; Bergan, RC
      Journal of the National Cancer Institute  101  1141-55  2009

      Show Abstract
      19638505 19638505
      Decoy receptor 3, upregulated by Epstein-Barr virus latent membrane protein 1, enhances nasopharyngeal carcinoma cell migration and invasion.
      Ho, CH; Chen, CL; Li, WY; Chen, CJ
      Carcinogenesis  30  1443-51  2009

      Show Abstract
      19483191 19483191
      Subcellular receptor redistribution and enhanced microspike formation by a Ret receptor preferentially recruiting Dok.
      Anna Stenqvist, T Kalle Lundgren, Matthew J Smith, Ola Hermanson, Gonçalo Castelo-Branco, Tony Pawson, Patrik Ernfors
      Neuroscience letters  435  11-6  2008

      Show Abstract
      18353552 18353552
      Role of MAPK phosphatase-1 in sustained activation of JNK during ethanol-induced apoptosis in hepatocyte-like VL-17A cells.
      Venugopal, SK; Chen, J; Zhang, Y; Clemens, D; Follenzi, A; Zern, MA
      The Journal of biological chemistry  282  31900-8  2007

      Show Abstract
      Western Blotting17848570 17848570
      WNT pathways in the neonatal ovine uterus: potential specification of endometrial gland morphogenesis by SFRP2.
      Hayashi, K; Spencer, TE
      Biology of reproduction  74  721-33  2006

      Show Abstract
      Immunohistochemistry (Paraffin)16407498 16407498