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05-598 Anti-phospho-Histone H3 (Ser10) Antibody, clone RR002

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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Vrt, NemICC, IHC, WBMPurifiedMonoclonal Antibody
      Catalogue Number05-598
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-Histone H3 (Ser10) Antibody, clone RR002
      Alternate Names
      • H3 histone, family 3A
      • H3S10P
      • Histone H3 (phospho S10)
      • H3 histone, family 3A
      • H3 histone, family 3B
      • H3 histone, family 3B (H3.3B)
      Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
      Product Information
      • UV-treated 293 cell extracts, UV-treated HeLa cell extracts, breast cancer tissue, HEPG2 cell extrats.
      PresentationPurified mouse IgG in buffer containing 0.1M Tris Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH 7.4.
      Quality LevelMQ100
      ApplicationAnti-phospho-Histone H3 (Ser10) Antibody, clone RR002 is a Mouse Monoclonal Antibody for detection of Histone H3 phosphorylated at serine 10. This purified mAb, also known as Anti-H3S10p, is published in peer reviewed journals and is validated in ICC, IHC, WB.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Western Blotting
      Applications Not Recommended
      • ELISA
      Application NotesELISA Analysis:
      This antibody showed no immuno-reactivity with an unphosphorylated Histone H3 peptide (residues 7-20), nor with other histone phosphopeptides.

      A 1:1000 dilution of this antibody detected mitotic cells in C. elegans embryo sections as shown by an independent laboratory.

      This lot showed positive staining of mitotic cells in cultures of HeLa cells undergoing exponential growth
      Biological Information
      ImmunogenPeptide (ARK[pS]TGGKAPRKQL-C) corresponding to amino acids 7-20 of human histone H3 with a c-terminal cyteine added for conjugation purposes.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody recognizes Histone H3 phosphorylated at Serine 10.
      Species Reactivity
      • Human
      • Vertebrates
      • Nematode
      Species Reactivity NoteProven to react with Human and C. elegans. Broad species cross-reactivity expected due to sequence conservation.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family.
      Gene Symbol
      • HIST1H3A
      • H3FA
      • HIST1H3B
      • H3FL
      • HIST1H3C
      • H3FC
      • HIST1H3D
      • H3FB
      • HIST1H3E
      • H3FD
      • HIST1H3F
      • H3FI
      • HIST1H3G
      • H3FH
      • HIST1H3H
      • H3FK
      • HIST1H3I
      • H3FF
      • HIST1H3J
      • H3FJ
      • HIST3H3
      • H3FT
      • H3T
      • MGC126886
      • Phosphorylation
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 136 amino acids; 15508 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity).
      SIMILARITY: SwissProt: Q16695 ## Belongs to the histone H3 family.
      Molecular Weight17 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by western blot on acid extracted proteins from colcemid-arrested HeLa cells.

      Western Blot Analysis:
      0.005-0.025 μg/mL of this lot detected phosphorylated Histone H3 in acid extracted proteins from colcemid-arrested HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Global Trade Item Number
      Catalog Number GTIN
      05-598 08436037123214


      Anti-phospho-Histone H3 (Ser10) Antibody, clone RR002 SDS


      Safety Data Sheet (SDS) 

      Anti-phospho-Histone H3 (Ser10) Antibody, clone RR002 Certificates of Analysis

      TitleLot Number
      Anti-phospho-Histone H3 (Ser10), clone RR002 - 2375607 2375607
      Anti-phospho-Histone H3 (Ser10), clone RR002 - 2455628 2455628
      Anti-phospho-Histone H3 (Ser10), clone RR002 - 20306 20306
      Anti-phospho-Histone H3 (Ser10), clone RR002 - 2032286 2032286
      Anti-phospho-Histone H3 (Ser10), clone RR002 - 2055510 2055510
      Anti-phospho-Histone H3 (Ser10), clone RR002 - 21884 21884
      Anti-phospho-Histone H3 (Ser10), clone RR002 - 2197497 2197497
      Anti-phospho-Histone H3 (Ser10), clone RR002 - 22980 22980
      Anti-phospho-Histone H3 (Ser10), clone RR002 - 2326375 2326375
      Anti-phospho-Histone H3 (Ser10), clone RR002 - 2517825 2517825


      Reference overviewApplicationPub Med ID
      Nuclear shape changes are induced by knockdown of the SWI/SNF ATPase BRG1 and are independent of cytoskeletal connections.
      Imbalzano, KM; Cohet, N; Wu, Q; Underwood, JM; Imbalzano, AN; Nickerson, JA
      PloS one  8  e55628  2013

      Show Abstract
      Immunofluorescence23405182 23405182
      Activating RNAs associate with Mediator to enhance chromatin architecture and transcription.
      Lai, F; Orom, UA; Cesaroni, M; Beringer, M; Taatjes, DJ; Blobel, GA; Shiekhattar, R
      Nature  494  497-501  2013

      Show Abstract
      23417068 23417068
      Light-activation of the Archaerhodopsin H(+)-pump reverses age-dependent loss of vertebrate regeneration: sparking system-level controls in vivo.
      Adams, DS; Tseng, AS; Levin, M
      Biology open  2  306-13  2013

      Show Abstract
      23519324 23519324
      Biochemical characterization of protein quality control mechanisms during disease progression in the C22 mouse model of CMT1A.
      Chittoor, VG; Sooyeon, L; Rangaraju, S; Nicks, JR; Schmidt, JT; Madorsky, I; Narvaez, DC; Notterpek, L
      ASN neuro  5  e00128  2013

      Show Abstract
      24175617 24175617
      Transmembrane potential of GlyCl-expressing instructor cells induces a neoplastic-like conversion of melanocytes via a serotonergic pathway.
      Blackiston, D; Adams, DS; Lemire, JM; Lobikin, M; Levin, M
      Disease models & mechanisms  4  67-85  2011

      Show Abstract
      Immunohistochemistry20959630 20959630
      EAPP: gatekeeper at the crossroad of apoptosis and p21-mediated cell-cycle arrest.
      Andorfer, P; Rotheneder, H
      Oncogene  30  2679-90  2011

      Show Abstract
      21258403 21258403
      The metazoan ATAC and SAGA coactivator HAT complexes regulate different sets of inducible target genes.
      Zita Nagy,Anne Riss,Sally Fujiyama,Arnaud Krebs,Meritxell Orpinell,Pascal Jansen,Adrian Cohen,Henk G Stunnenberg,Shigeaki Kato,Làszlò Tora
      Cellular and molecular life sciences : CMLS  67  2010

      Show Abstract
      19936620 19936620
      The spatial relationship between heterochromatin protein 1 alpha and histone modifications during mouse oocyte meiosis.
      Qiang Wang,Jun-Shu Ai,Safiriyu Idowu Ola,Ling Gu,Yong-Zhong Zhang,Da-Yuan Chen,Qing-Yuan Sun
      Cell cycle (Georgetown, Tex.)  7  2008

      Show Abstract
      18239470 18239470
      Drug-induced activation of dopamine D(1) receptor signaling and inhibition of class I/II histone deacetylase induce chromatin remodeling in reward circuitry and modulate cocaine-related behaviors.
      Schroeder, FA; Penta, KL; Matevossian, A; Jones, SR; Konradi, C; Tapper, AR; Akbarian, S
      Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology  33  2981-92  2008

      Show Abstract
      18288092 18288092
      Histone hyperacetylation occurs on promoters of lytic cycle regulatory genes in Epstein-Barr virus-infected cell lines which are refractory to disruption of latency by histone deacetylase inhibitors.
      Countryman, JK; Gradoville, L; Miller, G
      Journal of virology  82  4706-19  2008

      Show Abstract
      18337569 18337569

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      Life Science Research > Antibodies and Assays > Primary Antibodies