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05-636-AF647 Anti-phospho Histone H2A.X (Ser139) Antibody, clone JBW301, Alexa Fluor® 647

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05-636-AF647
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICCMAlexaFluor®647Monoclonal Antibody
      Description
      Catalogue Number05-636-AF647
      DescriptionAnti-phospho Histone H2A.X (Ser139) Antibody, clone JBW301, Alexa Fluor® 647
      Alternate Names
      • Histone H2AX
      • H2a/x
      • Histone H2A.X
      Background InformationAs a member of the histone H2A family, histone H2A.x (H2A.x) is a variant histone H2A which replaces conventional H2A in a subset of nucleosomes. H2A.x is involved in the DNA repair of double-strand breakage (DSB) damage on nuclear DNA. After a double strand DNA break, H2A.x is rapidly phosphorylated at serine 139 by ATM kinase and becomes gamma-H2AFX. This phosphorylation step can extend up to several thousand nucleosomes from the actual site of the DSB and may mark the surrounding chromatin for recruitment of proteins required for DNA damage signaling and repair. As a part of posttranslational modifications during apoptosis caused by severe DNA damage, high expression of phosphorylated H2A.x is considered as an accurate indicator of apoptosis.
      References
      Product Information
      FormatAlexaFluor®647
      PresentationPurified mouse conjugate in buffer containing PBS, 15 mg/ml BSA and 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-phospho Histone H2A.X (Ser139) Antibody, clone JBW301, Alexa Fluor® 647 is validated for use in ICC for the detection of phospho Histone H2A.X (Ser139).
      Key Applications
      • Immunocytochemistry
      Biological Information
      ImmunogenLinear peptide corresponding to phospho Histone H2A.X (Ser139).
      CloneJBW301
      Concentration0.5 mg/mL
      HostMouse
      IsotypeIgG1
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with vertebrates based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • H2AFX
      • H2AX
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~17 kDa observed. Refer to Cat. No. 05-636 for observed molecular weight information.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in untreated and staurosporin treated HeLa cells.

      Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected phospho Histone H2A.X (Ser139) in staurosporin treated HeLa cells.

      Alexa Fluor® is a registered trademark of Life Technologies®.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-phospho Histone H2A.X (Ser139) Antibody, clone JBW301, Alexa Fluor® 647 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-phospho Histone H2A.X (Ser139) Antibody, clone JBW301, Alexa Fluor® 647 Certificates of Analysis

      TitleLot Number
      Anti-phospho Histone H2A.X (Ser139), -2760520 2760520
      Anti-phospho Histone H2A.X (Ser139), -2816716 2816716
      Anti-phospho Histone H2A.X (Ser139), clone JBW301, Alexa Fluor® 647 3073304
      Anti-phospho Histone H2A.X (Ser139), clone JBW301, Alexa Fluor® 647 3082499
      Anti-phospho Histone H2A.X (Ser139), clone JBW301, Alexa Fluor® 647 2888554
      Anti-phospho Histone H2A.X (Ser139), clone JBW301, Alexa Fluor® 647 - 2555004
      Anti-phospho Histone H2A.X (Ser139), clone JBW301, Alexa Fluor® 647 - Q2430678 Q2430678
      Anti-phospho Histone H2A.X (Ser139), clone JBW301, Alexa Fluor® 647 - 3211145 3211145
      Anti-phospho Histone H2A.X (Ser139), clone JBW301, Alexa Fluor® 647 - 3288745 3288745
      Anti-phospho Histone H2A.X (Ser139), clone JBW301, Alexa Fluor® 647 - 3462127 3462127

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies