Millipore Sigma Vibrant Logo

05-636 Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301

View Products on Sigmaaldrich.com
05-636
200 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited Availability
Limited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      VrtICC, IF, WB, ChIP, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-636
      ReplacesMABE205
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301
      Alternate Names
      • H2AXS139P
      • Histone H2A.X (phospho S139)
      Background InformationHistone H2A is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the 'beads on a string' structure.
      References
      Product Information
      FormatPurified
      Control
      • UV-treated 293 cell extracts, UV-treated HeLa cell extracts or breast cancer tissue
      PresentationImmunoaffinity Purified immunoglobulin in 0.1M Tris-Glycine,pH 7.4, 0.15M NaCl, 0.05% sodium azide as a preservative.
      Quality LevelMQ100
      Applications
      ApplicationAnti-phospho-Histone H2A.X (Ser139), clone JBW301 is a well published Mouse Monoclonal Antibody validated in ChIP, ICC, IF, WB. This purified mAb is highly specific for phospho-Histone H2A.X (Ser139) also known as H2AXS139p.
      Key Applications
      • Immunocytochemistry
      • Immunofluorescence
      • Western Blotting
      • Chromatin Immunoprecipitation (ChIP)
      • Immunohistochemistry
      Application NotesAdditional Referenced Applications:
      Immunohistochemistry Analysis: A representative lot detected Histone H2A.X (pSer139) in RNF168-WT and RNF 168-SA/SEKI mice lung tissue sections (Paraffin). (Xe, X., et al. (2015) Nat. Cell Biol. 20 (3); 320-331).
      Chromatin Immunoprecipitation, see Meier, Andreas, et al. EMBO J., 26: 2707-18 (2007) in technical information tab.
      Biological Information
      Immunogenpeptide (C-KATQA[pS]QEY) corresponding to amino acids 134-142 of human histone H2A.X
      CloneJBW301
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes Histone H2A.X phosphorylated at Ser139.
      IsotypeIgG1
      Species Reactivity
      • Vertebrates
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.
      Gene Symbol
      • H2AFX
      • H2AX
      • H2a/x
      • H2A/X
      • H2A.X
      Modifications
      • Phosphorylation
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P16104 # Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C- terminal phosphorylation.
      SIZE: 143 amino acids; 15145 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Interacts with numerous proteins required for DNA damage signaling and repair when phosphorylated on Ser-140. These include MDC1, TP53BP1, BRCA1 and the MRN complex, composed of MRE11A, RAD50, and NBN. Interaction with the MRN complex is mediated at least in part by NBN. Also interacts with DHX9/NDHII when phosphorylated on Ser-140.
      SUBCELLULAR LOCATION: Nucleus.DEVELOPMENTAL STAGE: Synthesized in G1 as well as in S-phase.
      DOMAIN: SwissProt: P16104 The [ST]-Q motif constitutes a recognition sequence for kinases from the PI3/PI4-kinase family.
      PTM: Phosphorylated on Ser-140 (to form gamma-H2AFX) in response to DNA double strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks, and may also occur during meiotic recombination events and immunoglobulin class switching in lymphocytes. Phosphorylation can extend up to several thousand nucleosomes from the actual site of the DSB and may mark the surrounding chromatin for recruitment of proteins required for DNA damage signaling and repair. Widespread phosphorylation may also serve to amplify the damage signal or aid repair of persistent lesions. Phosphorylation of Ser-140 in response to ionizing radiation is mediated by both ATM and PRKDC while defects in DNA replication induce Ser-140 phosphorylation subsequent to activation of ATR and PRKDC. Dephosphorylation of Ser-140 by PP2A is required for DNA DSB repair. In meiosis, Ser-140 phosphorylation may occur at synaptonemal complexes during leptotene as an ATM-dependent response to the formation of programmed DSBs by SPO11. Ser-140 phosphorylation may subsequently occurs at unsynapsed regions of both autosomes and the XY bivalent during zygotene, downstream of ATR and BRCA1 activation. Ser-140 phosphorylation may also be required for transcriptional repression of unsynapsed chromatin and meiotic sex chromosome inactivation (MSCI), whereby the X and Y chromosomes condense in pachytene to form the heterochromatic XY-body. During immunoglobulin class switch recombination in lymphocytes, Ser-140 phosphorylation may occur at sites of DNA-recombination subsequent to activation of the activation-induced cytidine deaminase AICDA. & Monoubiquitination of Lys-120 by RING1 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression (By similarity).
      SIMILARITY: Belongs to the histone H2A family.
      Molecular Weight17 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceImmunoblot Analysis: 0.05-1 μg/ml of this antibody detected phosphorylated histone H2A.X (Ser139) in acid extracted histone lysates from Jurkat cells treated with 0.5 μM staurosporine (Catalog # 19-123).
      Immunocytochemistry: 2 μg/ml of this antibody detected phosphorylated histone H2A.X in HeLa cells treated with 0.5 μM staurosporine for 4-6 hours.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 1 year at 2 to 8°C from date of shipment. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      05-636 04053252588945

      Documentation

      Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301 Certificates of Analysis

      TitleLot Number
      Anti-phospho-Histone H2A.X (Ser139), -2739172 2739172
      Anti-phospho-Histone H2A.X (Ser139), -2789757 2789757
      Anti-phospho-Histone H2A.X (Ser139), -2806127 2806127
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301 2476967
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301 (mouse monoclonal IgG1) - DAM1405597 DAM1405597
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301 (mouse monoclonal IgG1) - DAM1474315 DAM1474315
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301 - 2116620 2116620
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301 - 2390526 2390526
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301 - 2392265 2392265
      Anti-phospho-Histone H2A.X (Ser139), clone JBW301 - 2420445 2420445

      References

      Reference overviewApplicationSpeciesPub Med ID
      Antitumor Activity of a 5-Hydroxy-1H-Pyrrol-2-(5H)-One-Based Synthetic Small Molecule In Vitro and In Vivo.
      Geng, Y; Wang, X; Yang, L; Sun, H; Wang, Y; Zhao, Y; She, R; Wang, MX; Wang, DX; Tang, J
      PloS one  10  e0128928  2015

      Show Abstract
      26042776 26042776
      Combined inhibition of the cell cycle related proteins Wee1 and Chk1/2 induces synergistic anti-cancer effect in melanoma.
      Magnussen, GI; Emilsen, E; Giller Fleten, K; Engesæter, B; Nähse-Kumpf, V; Fjær, R; Slipicevic, A; Flørenes, VA
      BMC cancer  15  462  2015

      Show Abstract
      26054341 26054341
      Activation of DNA Damage Response Pathways during Lytic Replication of KSHV.
      Hollingworth, R; Skalka, GL; Stewart, GS; Hislop, AD; Blackbourn, DJ; Grand, RJ
      Viruses  7  2908-27  2015

      Show Abstract
      26057167 26057167
      Streptococcus pneumoniae secretes hydrogen peroxide leading to DNA damage and apoptosis in lung cells.
      Rai, P; Parrish, M; Tay, IJ; Li, N; Ackerman, S; He, F; Kwang, J; Chow, VT; Engelward, BP
      Proceedings of the National Academy of Sciences of the United States of America  112  E3421-30  2015

      Show Abstract
      26080406 26080406
      Targeted DNA damage at individual telomeres disrupts their integrity and triggers cell death.
      Sun, L; Tan, R; Xu, J; LaFace, J; Gao, Y; Xiao, Y; Attar, M; Neumann, C; Li, GM; Su, B; Liu, Y; Nakajima, S; Levine, AS; Lan, L
      Nucleic acids research  43  6334-47  2015

      Show Abstract
      26082495 26082495
      AluY-mediated germline deletion, duplication and somatic stem cell reversion in UBE2T defines a new subtype of Fanconi anemia.
      Virts, EL; Jankowska, A; Mackay, C; Glaas, MF; Wiek, C; Kelich, SL; Lottmann, N; Kennedy, FM; Marchal, C; Lehnert, E; Scharf, RE; Dufour, C; Lanciotti, M; Farruggia, P; Santoro, A; Savasan, S; Scheckenbach, K; Schipper, J; Wagenmann, M; Lewis, T; Leffak, M; Farlow, JL; Foroud, TM; Honisch, E; Niederacher, D; Chakraborty, SC; Vance, GH; Pruss, D; Timms, KM; Lanchbury, JS; Alpi, AF; Hanenberg, H
      Human molecular genetics  24  5093-108  2015

      Show Abstract
      26085575 26085575
      The Effect of MicroRNA-124 Overexpression on Anti-Tumor Drug Sensitivity.
      Chen, SM; Chou, WC; Hu, LY; Hsiung, CN; Chu, HW; Huang, YL; Hsu, HM; Yu, JC; Shen, CY
      PloS one  10  e0128472  2015

      Show Abstract
      26115122 26115122
      SERBP1 affects homologous recombination-mediated DNA repair by regulation of CtIP translation during S phase.
      Ahn, JW; Kim, S; Na, W; Baek, SJ; Kim, JH; Min, K; Yeom, J; Kwak, H; Jeong, S; Lee, C; Kim, SY; Choi, CY
      Nucleic acids research  43  6321-33  2015

      Show Abstract
      26068472 26068472
      Prmt5 is required for germ cell survival during spermatogenesis in mice.
      Wang, Y; Zhu, T; Li, Q; Liu, C; Han, F; Chen, M; Zhang, L; Cui, X; Qin, Y; Bao, S; Gao, F
      Scientific reports  5  11031  2015

      Show Abstract
      26072710 26072710
      Atrazine Triggers DNA Damage Response and Induces DNA Double-Strand Breaks in MCF-10A Cells.
      Huang, P; Yang, J; Ning, J; Wang, M; Song, Q
      International journal of molecular sciences  16  14353-68  2015

      Show Abstract
      26114388 26114388

      Technical Info

      Title
      White Paper - The Message in the Marks: Deciphering Cancer Epigenetics

      FAQ

      QuestionAnswer
      How can I check the phosphospecificity of my antibody once my protein is already immobilized on a membrane?You can check for the phosphospecificity of your antibody using Lambda Protein Phosphatase. Simply perform SDS-polyacrylamide gel electrophoresis (SDS-PAGE) on a cell lysate and transfer the proteins to your membrane of choice. Wash the blotted nitrocellulose twice with water. Block the blotted nitrocellulose in freshly prepared TBS containing 1% bovine serum albumin (BSA) and 0.1% Triton X-100 for 1 hour at 20-25°C with constant agitation. Incubate the nitrocellulose in TBS containing 1% bovine serum albumin (BSA), 0.1% Triton X-100 and 2 mM MnCl2, and where dephosphorylation of proteins is desirable, 400 U/ml Lambda Protein Phosphatase for two hours at room temperature, or overnight at 4°C. After incubation, wash the nitrocellulose in PBS-0.1% Tween 20 for 3-5 minutes. Rinse the nitrocellulose in 4-5 changes of water. Continue with your western blotting assay.

      Related Products & Applications

      Related Products

      Alternative Packsize

      Catalog Number Description  
      05-636-KC Anti-Phos-Hist H2A.1 (S139) (KC) Show Pricing & Availability

      Alternative Format

      Catalog Number Description  
      16-193 Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301, biotin conjugate Show Pricing & Availability
      16-202A Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301, FITC conjugate Show Pricing & Availability

      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies