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07-681 Anti-phospho-AMPK α (Thr172) Antibody

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07-681
200 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RWBRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number07-681
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-AMPK α (Thr172) Antibody
      References
      Product Information
      FormatPurified
      Control
      • Serum starved HEK293 cells
      PresentationProtein A Purified rabbit IgG in a solution of 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.135M NaCl, and 0.035% sodium azide as a preservative.
      Quality LevelMQ100
      Applications
      ApplicationAnti-phospho-AMPK α (Thr172) Antibody is an antibody against phospho-AMPK α (Thr172) for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide corresponding to amino acids surrounding phosphorylated Thr172 of human AMP-activated protein kinase alpha 2 (AMPKα2)
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes phospho-AMPKα.
      IsotypeIgG
      Species Reactivity
      • Human
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
      Gene Symbol
      • PRKAA1
      • MGC57364
      • AMPK
      • MGC33776
      • AMPKa1
      • AMPK1
      Modifications
      • Phosphorylation
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q13131 # Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.
      COFACTOR: Magnesium.
      SIZE: 550 amino acids; 62808 Da
      SUBUNIT: Heterotrimer of an alpha catalytic subunit, a beta and a gamma non-catalytic subunits. Interacts with FNIP1.
      SIMILARITY: SwissProt: Q13131 ## Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily. & Contains 1 protein kinase domain.
      Molecular WeightMr 63 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates from overnight starved HEK293 cells and partially purified rat liver AMPK
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-phospho-AMPK α (Thr172) Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-phospho-AMPK α (Thr172) Antibody Certificates of Analysis

      TitleLot Number
      Anti-phospho-AMPK alpha; (Thr172) - 30346 30346
      Anti-phospho-AMPK#945; (Thr172) (rabbit polyclonal IgG) - 2117737 2117737
      Anti-phospho-AMPK#945; (Thr172) (rabbit polyclonal IgG) - DAM1813211 DAM1813211
      Anti-phospho-AMPKa (Thr172) (rabbit polyclonal IgG) 2901522
      Anti-phospho-AMPKa (Thr172) (rabbit polyclonal IgG) - 2193168 2193168
      Anti-phospho-AMPKa (Thr172) (rabbit polyclonal IgG) - 2325758 2325758
      Anti-phospho-AMPKa (Thr172) - 1984819 1984819
      Anti-phospho-AMPKa (Thr172) - DAM1564575 DAM1564575
      Anti-phospho-AMPKa (Thr172) - DAM1614931 DAM1614931
      Anti-phospho-AMPKa (Thr172) - DAM1767495 DAM1767495

      References

      Reference overviewApplicationSpeciesPub Med ID
      Enhanced external counterpulsation improves peripheral artery function and glucose tolerance in subjects with abnormal glucose tolerance.
      Martin, JS; Beck, DT; Aranda, JM; Braith, RW
      Journal of applied physiology (Bethesda, Md. : 1985)  112  868-76  2012

      Show Abstract
      Western BlottingHuman22194326 22194326
      Sending the signal: molecular mechanisms regulating glucose uptake.
      Krook, Anna, et al.
      Medicine and science in sports and exercise, 36: 1212-7 (2004)  2004

      Show Abstract
      15235328 15235328
      Adiponectin, obesity, and cardiovascular disease.
      Fasshauer, Mathias, et al.
      Biochimie, 86: 779-84 (2004)  2004

      Show Abstract
      15589686 15589686
      AMP-activated protein kinase, super metabolic regulator.
      Kemp, B E, et al.
      Biochem. Soc. Trans., 31: 162-8 (2003)  2003

      Show Abstract
      12546677 12546677
      Roles of 5'-AMP-activated protein kinase (AMPK) in mammalian glucose homoeostasis.
      Rutter, Guy A, et al.
      Biochem. J., 375: 1-16 (2003)  2003

      Show Abstract
      12839490 12839490
      AMPK as a metabolic switch in rat muscle, liver and adipose tissue after exercise.
      Ruderman, N B, et al.
      Acta Physiol. Scand., 178: 435-42 (2003)  2003

      Show Abstract
      12864749 12864749
      Transgenic models--a scientific tool to understand exercise-induced metabolism: the regulatory role of AMPK (5'-AMP-activated protein kinase) in glucose transport and glycogen synthase activity in skeletal muscle.
      Wojtaszewski, J F P, et al.
      Biochem. Soc. Trans., 31: 1290-4 (2003)  2003

      Show Abstract
      14641045 14641045

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      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies