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MABN328 Anti-peptidyl-citrulline, clone F95 Antibody

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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, Ch, RIHC, WB, ICCMPurifiedMonoclonal Antibody
      Catalogue NumberMABN328
      DescriptionAnti-peptidyl-citrulline, clone F95 Antibody
      Alternate Names
      • Peptidyl-citrulline
      • Protein-bound citrulline
      Background InformationDeimination (citrullination) is a post translational modification in which the peptidyl-arginine deiminase family of enzymes convert the guanidino side chain of the amino acid arginine into a ureido group, yeilding peptidyl-citrulline. As a result, anti-peptidyl-citrulline antibodies should also cross react with peptidyl-homocitrulline, produced by the carbamylation of lysine, but not free citrulline, which is created in the urea cycle, or as a by-product of nitric oxide synthesis.
      Product Information
      PresentationPurified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.
      Quality LevelMQ100
      ApplicationThis Anti-peptidyl-citrulline, clone F95 Antibody is validated for use in Immunohistochemistry and Western Blotting and Immunocytochemistry for the detection of peptidyl-citrulline.
      Key Applications
      • Immunohistochemistry
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunohistochemistry Analysis: A 1:250 dilution from a representative lot detected peptidyl-citrulline in human cerebellum, and rat cerebellum tissue.
      Western Blotting Analysis: A representative lot detected peptidyl-citrulline in CNS proteins from brains and spinal cord of EAE mice (Nicholas et al., 2005).
      Biological Information
      ImmunogenCitrullinated peptide consisting of 10 citrulline residues and a carboxyl Gly-Gly-Cys.
      ConcentrationPlease refer to lot specific datasheet.
      Species Reactivity
      • Human
      • Mouse
      • Chicken
      • Rat
      Species Reactivity NotePredicted to react with All based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Purification MethodPurified by Ion-Exchange Chromatography
      Molecular WeightVarious kDa calculated
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in human cerebral cortex tissue.

      Imunnohistochemistry Analysis: A 1:250 dilution of this antibody detected peptidyl-citrulline inhuman cerebral cortex tissue.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information


      Anti-peptidyl-citrulline, clone F95 Antibody SDS


      Safety Data Sheet (SDS) 

      Anti-peptidyl-citrulline, clone F95 Antibody Certificates of Analysis

      TitleLot Number
      Anti-peptidyl-citrulline, clone F95 2485461
      Anti-peptidyl-citrulline, clone F95 3058401
      Anti-peptidyl-citrulline, clone F95 - Q2463716 Q2463716
      Anti-peptidyl-citrulline, clone F95 - 2517374 2517374
      Anti-peptidyl-citrulline, clone F95 - 2564054 2564054
      Anti-peptidyl-citrulline, clone F95 - 3245641 3245641
      Anti-peptidyl-citrulline, clone F95 - 3313127 3313127
      Anti-peptidyl-citrulline, clone F95 - 3430321 3430321
      Anti-peptidyl-citrulline, clone F95 - 3519866 3519866
      Anti-peptidyl-citrulline, clone F95 - 3724741 3724741


      Reference overviewPub Med ID
      Dual immunofluorescence study of citrullinated proteins in Parkinson diseased substantia nigra.
      Nicholas, Anthony P
      Neurosci. Lett., 495: 26-9 (2011)  2011

      Show Abstract
      21414385 21414385
      Expression of citrullinated proteins in murine experimental autoimmune encephalomyelitis.
      Nicholas, Anthony P, et al.
      J. Comp. Neurol., 486: 254-66 (2005)  2005

      Show Abstract
      15844173 15844173
      Synovial intracellular citrullinated proteins colocalizing with peptidyl arginine deiminase as pathophysiologically relevant antigenic determinants of rheumatoid arthritis-specific humoral autoimmunity.
      De Rycke, Leen, et al.
      Arthritis Rheum., 52: 2323-30 (2005)  2005

      Show Abstract
      16052592 16052592
      Preparation of a monoclonal antibody to citrullinated epitopes: its characterization and some applications to immunohistochemistry in human brain.
      Nicholas, Anthony P and Whitaker, John N
      Glia, 37: 328-36 (2002)  2002

      Show Abstract
      11870872 11870872