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MABN54 Anti-pan-SAPAP Antibody, clone N127/31

MABN54
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      RIHC, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN54
      DescriptionAnti-pan-SAPAP Antibody, clone N127/31
      Alternate Names
      • discs, large (Drosophila) homolog-associated protein 1
      • SAP90/PSD-95-associated protein 1
      • disks large-associated protein 1
      • Guanylate kinase-associated protein
      • PSD-95/SAP90 binding protein 1
      • PSD-95/SAP90-binding protein 1
      Background InformationSAPAP1 is a member of the SAPAP family of proteins and is thought to be instrumental in the postsynaptic scaffold of neurons. SAPAP1 may function to assemble various other proteins at synapses. These functional proteins include scaffolding proteins, signaling proteins and cytoskeletal proteins. SAPAP1 is primarily found in the brain and more specifically in the cell body zones.
      References
      Product Information
      FormatPurified
      Control
      • Rat brain membrane tissue lysate
      PresentationPurified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationThis Anti-pan-SAPAP Antibody, clone N127/31 is validated for use in IH, WB for the detection of pan-SAPAP.
      Key Applications
      • Immunohistochemistry
      • Western Blotting
      Application NotesImmunohistochemistry Analysis: 1:400 dilution from a previous lot detected SAPAP in rat schwan cells (putamen area) and rat dopaminergic neurons.

      Immunofluorescence Analysis: A previous lot of this antibody was used by an independent laboratory in cultured rat hippocampal pyramidal cells and interneurons. (Courtesy of Dieter Edbauer and Morgan Sheng, Picower Institute, MIT.)
      Biological Information
      ImmunogenRecombinant protein corresponding to rat SAPAP.
      CloneN127/31
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG2bκ
      Species Reactivity
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene Summarybinds the guanylate kinase-like domain of the PSD-95 family of synaptic ion channel clustering proteins; may play a role in protein-protein interactions at the synaptic junction [RGD].
      Gene Symbol
      • DLGAP1
      • GKAP
      • DAP-1-BETA
      • DAP-1
      • SAPAP1
      • DAP-1-ALPHA
      • hGKAP
      • DAP1
      Purification MethodProtein G
      UniProt Number
      UniProt SummaryFUNCTION: Part of the postsynaptic scaffold in neuronal cells.

      SUBUNIT STRUCTURE: Interacts with guanylate kinase-like domain of DLG1, DLG2, DLG3, DLG4 and AIP1. Interacts with the PDZ domain of SHANK1, SHANK2 and SHANK3. Found in a complex with DLG4 and SHANK1, SHANK2 or SHANK3. Found in a complex with DLG4 and BEGAIN. Interacts with DYL2 and LRFN1.

      SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein. Cell junction › synapse › postsynaptic cell membrane › postsynaptic density. Cell junction › synapse. Note: Found in postsynaptic density of neuronal cells.

      TISSUE SPECIFICTY: Expressed in brain and testis.

      DEVELOPMENTAL STAGE: Highest level of isoform 1 in the brain of newborn rats. Increasing levels of isoforms 2, 3, 4, and 5 in the brain of newborn rats from birth to 6 weeks of postnatal development. Increasing but low level of isoform 6 is expressed in the brain from 2 to 6 weeks of postnatal development.

      SEQUENCE SIMILARITIES: Belongs to the SAPAP family.
      Molecular WeightIsoforms between ~90-120 kDa observed. Other isoforms may be observed in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in rat brain membrane tissue lysate.

      Western Blot Analysis: 2 µg/mL of this antibody detected SAPAP on 10 µg of rat brain membrane tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications