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MABE327 Anti-p53 (pantropic) Antibody, clone DO-1

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MABE327
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICC, IHC, IF, IPMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE327
      Replaces04-1083
      DescriptionAnti-p53 (pantropic) Antibody, clone DO-1
      Alternate Names
      • Cellular tumor antigen p53
      • Antigen NY-CO-13
      • Phosphoprotein p53
      • Tumor suppressor p53
      Background InformationCellular tumor antigen p53 (p53) is a ubiquitous transcription factor that is most widely known for its function as a tumor suppressor, through its regulation of cell growth and survival. p53 is activated in response to cell stress such as DNA damage and induces either cell cycle arrest or apoptosis. It coordinates the expression of multiple genes including p21, GADD45, Bax, and Puma. p53 is regulated by multiple mechanisms: At the post-transcriptional level, the stability of p53 mRNAs is regulated by the binding of molecules such as HuR, Wig-1, RPL26, L26, and WRAP53. Post-translationally, p53 undergoes phosphorylation and acetylation which may also play a role in regulating its activity. It may also undergo ubiquitination via the Akt-MDM2 pathway resulting in degradation by the 20S proteosome. Mutations in p53 are prevalent in many types of cancers, underscoring the therapeutic relevance of this protein.
      References
      Product Information
      FormatPurified
      Control
      • A431 cell lysate
      PresentationPurified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationUse Anti-p53 (pantropic) Antibody, clone DO-1 (Mouse Monoclonal Antibody) validated in WB, ICC, IHC, IF, IP to detect p53 (pantropic) also known as Cellular tumor antigen p53 or Antigen NY-CO-13.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunofluorescence
      • Immunoprecipitation
      Application NotesImmunocytochemistry Analysis: A 1:500 dilution from a representative lot detected p53 (pantropic) in A431 cells.

      Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected p53 (pantropic) in human colorectal adenocarcinoma tissue and in human prostate cancer tissue.

      Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected p53 (pantropic) in human colorectal cancer cells.

      Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in radiolabelled HOS cell lysate (Vojtesek, B., et al. (1995). 71(6):1253-1256.).

      Immunohistochemistry Analysis: A representative lot was used by an independent laboratory in primary breast carcinoma tissue (Beck, T., et al. (1995). Gynecol Oncol. 57(1):96-104.).
      Biological Information
      ImmunogenRecombinant protein corresponding to human p53 (pantropic).
      EpitopeN-terminus
      CloneDO-1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes the N-terminus of p53 (pantropic).
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of this gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome. Multiple p53 variants due to alternative promoters and multiple alternative splicing have been found. These variants encode distinct isoforms, which can regulate p53 transcriptional activity.
      Gene Symbol
      • TP53
      • P53
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~53 kDa observed. Uniprot describes 9 different isoforms due to alternative splicing and post-translational modification. This antibody detects isoform 1 (~38 kDa), isoform 2 (~39 kDa), and isoform 3 (~39 kDa). An uncharacterized band may be observed at ~43 kDa in some cell lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in A431 cell lysate.

      Western Blot Analysis: 1 µg/mL of this antibody detected p53 (pantropic) in 10 µg of A431 cell lysate. This antibody detects isoforms 1, 2, and 3.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-p53 (pantropic) Antibody, clone DO-1 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-p53 (pantropic) Antibody, clone DO-1 Certificates of Analysis

      TitleLot Number
      Anti-p53 (pantropic), clone DO-1 2480947
      Anti-p53 (pantropic), clone DO-1 - 2146023 2146023
      Anti-p53 (pantropic), clone DO-1 - 2424840 2424840
      Anti-p53 (pantropic), clone DO-1 - 2505480 2505480
      Anti-p53 (pantropic), clone DO-1 - 3277579 3277579
      Anti-p53 (pantropic), clone DO-1 - 3378721 3378721
      Anti-p53 (pantropic), clone DO-1 - 3639361 3639361
      Anti-p53 (pantropic), clone DO-1 - Q2005659 Q2005659
      Anti-p53 (pantropic), clone DO-1 -2594441 2594441
      Anti-p53 (pantropic), clone DO-1 -2677926 2677926

      References

      Reference overviewPub Med ID
      Usefulness of immunohistochemical staining for p53 in the prognosis of breast carcinomas: correlations with established prognosis parameters and with the proliferation marker, MIB-1.
      Beck, T, et al.
      Gynecol. Oncol., 57: 96-104 (1995)  1995

      Show Abstract
      7705708 7705708
      Absence of p53 autoantibodies in a significant proportion of breast cancer patients.
      Vojtesek, B, et al.
      Br. J. Cancer, 71: 1253-6 (1995)  1995

      Show Abstract
      7779720 7779720

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies