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CBL404 Anti-p53 Antibody, aa 211-220, clone240

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CBL404
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, Ch, H, Ht, M, Mk, RIP, WB, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberCBL404
      Replaces04-1083
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-p53 Antibody, aa 211-220, clone240
      Background Informationp53 was discovered in 1979 as a cellular protein associating with the transforming protein of SV40 tumor virus. Since then, many different biochemical functions have been attributed to the 53 kD phosphoprotein. Experimental evidence has suggested that p53 acts as a negative regulator of cell growth in normal cells (Finlay, 1989). Thus, the inactivation or mutation of p53 may be an essential step in the development of malignancy (Lane and Benchmol, 1990). Wild-type p53 levels in normal cells and tissues were found to be very low. Mutant p53 polypeptide, however, is often found to be present at high concentrations in mammalian tumors and tumor cell lines. For example, in an immuno-histochemistry study 40% of human breast cancer showed elevated levels of mutant p53 in the cell nucleus. Mutations of the p53 protein have some characteristic features:

      a) Most of them are missense point mutations giving rise to an altered protein function.

      b) Many -but not all- mutant p53 proteins exhibit a common mutant structure, which can be recognized by monoclonal antibodies specific for p53 in the mutant conformation.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal in buffer containing 0.1M Tris-glycine (pH 7.4), 0.15M NaCl, with 0.05% sodium azide. We recommend that each laboratory determine an optimum working titre for use in its particular application.
      Quality LevelMQ100
      Applications
      ApplicationDetect p53 with Anti-p53 Antibody, aa 211-220, clone240 (Mouse Monoclonal Antibody), that has been shown to work in IP, WB & IHC.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • Immunohistochemistry
      Applications Not Recommended
      • Immunohistochemistry (Paraffin)
      Application NotesDetection of p53 oncogene protein

      Detection of mutant p53

      Prevalence of detection using CBL 404

      -50% colon carcinoma sections positive (30 samples)

      -70% lung carcinoma sections positive (50 samples)

      -30% carcinoma breast samples positive (50 samples)

      Normal and pre-malignant tissues negative

      Reacts on methacarn fixed tissue

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenMurine p53-beta galactosidase fusion protein expressed in E. coli.
      Epitopeaa 211-220
      ClonePAb240
      HostMouse
      SpecificityPAb 240 recognizes an epitope of p53 tumor suppressor protein between amino acids 211 and 220 (Gannon, 1990; Legros, 1994) in human, mouse, rat, hamster, monkey, cow and chicken. Assaying native samples (immunoprecipitation, ELISA) the PAb 240 detects only the mutant forms of p53 (Gannon, 1990; Said, 1994). In methods using denatured samples [Western blot analysis (Gannon, 1990) and immunohistochemistry of frozen tissue sections (Said, 1994; Bartek, 1991; Walker, 1991)], the PAb 240 recognizes both mutant and denatured wild-type p53.
      IsotypeIgG1
      Species Reactivity
      • Bovine
      • Chicken
      • Human
      • Hamster
      • Mouse
      • Monkey
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryTumor protein p53, a nuclear protein, plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. It is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing DNA-binding, oligomerization and transcription activation domains. It is postulated to bind as a tetramer to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of the TP53 gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome.
      Gene Symbol
      • TP53
      • TRP53
      • P53
      • p53
      • LFS1
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P04637 # Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression.
      COFACTOR: Binds 1 zinc ion per subunit.
      SIZE: 393 amino acids; 43653 Da
      SUBUNIT: Interacts with AXIN1. Probably part of a complex consisting of TP53, HIPK2 and AXIN1 (By similarity). Binds DNA as a homotetramer. Interacts with histone acetyltransferases EP300 and methyltransferases HRMT1L2 and CARM1, and recruits them to promoters. In vitro, the interaction of TP53 with cancer- associated/HPV (E6) viral proteins leads to ubiquitination and degradation of TP53 giving a possible model for cell growth regulation. This complex formation requires an additional factor, E6-AP, which stably associates with TP53 in the presence of E6. C- terminus interacts with TAF1, when TAF1 is part of the TFIID complex. Interacts with ING4 and this interaction may be indirect. Found in a complex with CABLES1 and TP73. Interacts with HIPK1, HIPK2, and P53DINP1. Interacts with WWOX. May interacts with HCV core protein. Interacts with USP7 and SYVN1. Interacts with HSP90AB1 (By similarity). Interacts with BANP.
      SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Endoplasmic reticulum. Note=Interaction with BANP promotes nuclear localization.
      DOMAIN: SwissProt: P04637 The nuclear export signal acts as a transcriptional repression domain.
      PTM: Acetylated. Acetylation of Lys-382 by CREBBP enhances transcriptional activity. Deacetylation of Lys-382 by SIRT1 impairs its ability to induce proapoptotic program and modulate cell senescence. & Phosphorylation on Ser residues mediates transcriptional activation. Phosphorylated by HIPK1 (By similarity). Phosphorylated on Thr-18 by VRK1, which may prevent the interaction with MDM2. Phosphorylated on Thr-55 by TAF1, which promotes MDM2-mediated degradation. Phosphorylated on Ser-46 by HIPK2 upon UV irradiation. Phosphorylation on Ser-46 is required for acetylation by CREBBP. Phosphorylated on Ser-392 following UV but not gamma irradiation. Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylated on Ser-15 upon ultraviolet irradiation; which is enhanced by interaction with BANP. & Dephosphorylated by PP2A. SV40 small T antigen inhibits the dephosphorylation by the AC form of PP2A. & May be O-glycosylated in the C-terminal basic region. Studied in EB-1 cell line. & Ubiquitinated by SYVN1, which leads to proteasomal degradation.
      DISEASE: SwissProt: P04637 # TP53 is found in increased amounts in a wide variety of transformed cells. TP53 is frequently mutated or inactivated in about 60% of cancers. & Defects in TP53 are involved in esophageal squamous cell carcinoma (ESCC) [MIM:133239]. ESCC is a tumor of the esophagus. & Defects in TP53 are a cause of Li-Fraumeni syndrome (LFS) [MIM:151623]. LFS is an autosomal dominant familial cancer syndrome that in its classic form is defined by the existence of both a proband with a sarcoma and two other first-degree relatives with a cancer by age 45 years. In these families the affected relatives develop a diverse set of malignancies at unusually early ages. The spectrum of cancers in LFS includes breast carcinomas, soft-tissue sarcomas, brain tumors, osteosarcoma, leukemia and adreno-cortical carcinoma. Other possible component tumors of LFS are melanoma, gonadal cell tumors and carcinomas of the lung, pancreas and prostate. & Defects in TP53 may be associated with nasopharyngeal carcinoma [MIM:161550]; also known as nasopharyngeal cancer. & Defects in TP53 are found in Barrett metaplasia; also known as Barrett esophagus. It is a condition in which the normally stratified squamous epithelium of the lower esophagus is replaced by a metaplastic columnar epithelium. The condition develops as a complication in approximately 10% of patients with chronic gastroesophageal reflux disease and predisposes to the development of esophageal adenocarcinoma. & Defects in TP53 are involved in head and neck squamous cell carcinomas (HNSCC) [MIM:275355]. & Defects in TP53 are involved in oral squamous cell carcinoma (OSCC). Cigarette smoke is a prime mutagenic agent in cancer of the aerodigestive tract. & Defects in TP53 are a cause of lung cancer [MIM:211980]. & Defects in TP53 are a cause of choroid plexus papilloma [MIM:260500]. Choroid plexus papilloma is a slow-growing benign tumor of the choroid plexus that often invades the leptomeninges. In children it is usually in a lateral ventricle but in adults it is more often in the fourth ventricle. Hydrocephalus is common, either from obstruction or from tumor secretion of cerebrospinal fluid. If it undergoes malignant transformation it is called a choroid plexus carcinoma. Primary choroid plexus tumors are rare and usually occur in early childhood. & Defects in TP53 are a cause of one form of hereditary adrenocortical carcinoma (ADCC) [MIM:202300]. ADCC is a rare childhood tumor, representing about 0.4% of childhood tumors, with a high incidence of associated tumors. ADCC occurs with increased frequency in patients with the Beckwith-Wiedemann syndrome [MIM:130650] and is a component tumor in Li-Fraumeni syndrome [MIM:151623].
      SIMILARITY: Belongs to the p53 family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsFor use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-p53 Antibody, aa 211-220, clone240 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-p53 Antibody, aa 211-220, clone240 Certificates of Analysis

      TitleLot Number
      Anti-p53, aa 211-220, clone PAb240 2475754
      Anti-p53, aa 211-220, clone PAb240 - 2204910 2204910
      Anti-p53, aa 211-220, clone PAb240 - 2273812 2273812
      Anti-p53, aa 211-220, clone PAb240 - 2344658 2344658
      Anti-p53, aa 211-220, clone PAb240 - 3013380 3013380
      Anti-p53, aa 211-220, clone PAb240 - 3158855 3158855
      Anti-p53, aa 211-220, clone PAb240 - 3365609 3365609
      Anti-p53, aa 211-220, clone PAb240 - 3472438 3472438
      Anti-p53, aa 211-220, clone PAb240 - 3557799 3557799
      Anti-p53, aa 211-220, clone PAb240 - 3679452 3679452

      References

      Reference overviewApplicationPub Med ID
      Clld7, a candidate tumor suppressor on chromosome 13q14, regulates pathways of DNA damage/repair and apoptosis.
      Zhou, X; Münger, K
      Cancer research  70  9434-43  2010

      Show Abstract
      Western Blotting20926398 20926398
      The amino-terminal transforming region of simian virus 40 large T and small t antigens functions as a J domain
      Srinivasan, A, et al
      Mol Cell Biol, 17:4761-73 (1997)  1997

      9234732 9234732
      Linear antigenic sites defined by the B-cell response to human p53 are localized predominantly in the amino and carboxy-termini of the protein.
      Legros, Y, et al.
      Oncogene, 9: 2071-6 (1994)  1994

      Show Abstract
      8208553 8208553
      Activating mutations in p53 produce a common conformational effect. A monoclonal antibody specific for the mutant form.
      Gannon, J V, et al.
      EMBO J., 9: 1595-602 (1990)  1990

      Show Abstract
      1691710 1691710
      Increased expression of mutant forms of p53 oncogene in primary lung cancer.
      Iggo, R, et al.
      Lancet, 335: 675-9 (1990)  1990

      Show Abstract
      1969059 1969059

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      Life Science Research > Antibodies and Assays > Primary Antibodies