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07-1570 Anti-monomethyl Histone H4 (Lys20) Antibody

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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, DB, ChIP, PIA Rb Affinity Purified Polyclonal Antibody
      Catalogue Number07-1570
      DescriptionAnti-monomethyl Histone H4 (Lys20) Antibody
      Alternate Names
      • H4K20me1
      • Histone H4 (mono methyl K20)
      • H4 histone family, member A
      • histone 1
      • H4a
      • histone cluster 1
      • H4a
      Background InformationHistones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. Histones modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. These modifications can alter local chromatin architecture, or recruit trans-acting factors that recognize specific histone modifications (the "histone code" hypothesis). Methylation of histone H4 at lysine 20 has been implicated in transcriptional activation, gene silencing, heterochromatin formation, mitosis, and DNA repair. Histone H4 is progressively methylated at Lys20 during the G(2), M, and G(1) phases of the cell cycle, and methylation of H4Lys20 is a marker for heterochromatin. In mammals, the female X chromosome is coated by the Xist RNA, which is critical for silencing, and is marked mainly by methylation of H3Lys9, H3Lys27 and H4Lys20.
      Product Information
      FormatAffinity Purified
      • HeLa cell acid extracts.
      PresentationPurified from serum by affinity chromatography. Supplied at 1 mg/ml in 0.1M Tris-Glycine (pH7.4), 15mM NaCl, 0.05% NaN3.
      Quality LevelMQ100
      ApplicationAnti-monomethyl Histone H4 (Lys20) Antibody is a rabbit polyclonal antibody for detection of monomethyl Histone H4 (Lys20) also known as H4K20me1, Histone H4 (mono methyl K20), H4 histone family member A & has been validated in WB.
      Key Applications
      • Western Blotting
      • Dot Blot
      • Chromatin Immunoprecipitation (ChIP)
      • Peptide Inhibition Assay
      Application NotesChromatin Immunoprecipitation:
      Sonicated chromatin prepared from HeLa cells (2 X 106 cell equivalents per IP) was subjected to chromatin immunoprecipitation using 4 μg of either a negative control antibody or Anti-Monomethyl-Histone H4
      (Lys20) antibody and the Magna ChIP A Kit (Cat. #17-610). Successful immunoprecipitation of monomethyl-histone H4 (Lys20)-associated DNA fragments was verified by qPCR using GAPDH coding region ChIP Primers versus Control Primers corresponding to the GAPDH promoter (Please see figures). Data is presented as percent input of each IP sample relative to input chromatin, with immunoprecipitated DNA from negative control antibody shown as (-) and monomethyl-histone H4 (Lys20) shown as (+).
      Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.

      Dot Blot Analysis :
      Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665), which contain histone peptides with various modifications were probed with Cat. No. 07-1570 Anti-monomethyl Histone H4 (Lys20) at 1:1000 dilution. Proteins were visualized using a Donkey anti-rabbit IgG conjugated to HRP and a chemiluminescence detection system.

      Peptide Blocking Assay:
      40 μg of histone H4 peptide containing monomethyl lysine 20 abolished detection of histone H4 by anti-monomethyl-Histone H4 (Lys20) in immunoblot analysis of acid extracts of HeLa cells
      Biological Information
      ImmunogenKLH-conjugated, linear synthetic peptide corresponding to amino acids 15-24
      (AKRHR[me1K]VLRD-C) of human Histone H4.
      EpitopeMonomethyl Lys20
      SpecificityRecognizes monomethylated Histone H4
      (Lys20), Mr 11 kDa.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Based on sequence homology, broad species cross-reactivity is expected
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq]
      Gene Symbol
      • H4/A
      • H4/B
      • H4/C
      • H4/D
      • H4/E
      • H4/G
      • H4/H
      • H4/I
      • H4/J
      • H4/K
      • H4/M
      • H4/N
      • H4/O
      • H4/a
      • H4F2
      • H4FA
      • H4FB
      • H4FC
      • H4FD
      • H4FE
      • H4FG
      • H4FH
      • H4FI
      • H4FJ
      • H4FK
      • H4FM
      • H4FN
      • H4FO
      • HIST2H4
      • Methylation
      Purification MethodAffinity Chromatography
      UniProt Number
      UniProt SummaryFUNCTION: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA

      PTM: Acetylation at Lys-6, Lys-9, Lys-13 and Lys-17 occurs in coding regions of the genome but not in heterochromatin.
      PTM: Citrullination at Arg-4 by PADI4 impairs methylation.
      PTM: Monomethylation at Arg-4 by PRMT1 favors acetylation at Lys-9 and Lys-13. Demethylation is performed by JMJD6.
      PTM: Monomethylated, dimethylated or trimethylated at Lys-21. Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
      PTM: Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.
      PTM: Sumoylated, which is associated with transcriptional repression.
      SIMILARITY: Belongs to the histone H4 family.
      Molecular Weight~11 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by western blot analysis
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information


      Anti-monomethyl Histone H4 (Lys20) Antibody Certificates of Analysis

      TitleLot Number
      Anti- monomethyl Histone H4 (Lys20)2483962
      Anti- monomethyl Histone H4 (Lys20) - 33289913328991
      Anti- monomethyl Histone H4 (Lys20) -25670162567016
      Anti- monomethyl Histone H4 (Lys20) -27968262796826
      Anti- monomethyl Histone H4 (Lys20) Polyclonal Antibody2941503
      Anti-monomethyl Histone H4 (Lys20) - 21727862172786
      Anti-monomethyl Histone H4 (Lys20) - 22792652279265
      Anti-monomethyl Histone H4 (Lys20) - NG1547654NG1547654
      Anti-monomethyl Histone H4 (Lys20) - NG1894392NG1894392


      Reference overviewApplicationPub Med ID
      Radiation-induced alterations of histone post-translational modification levels in lymphoblastoid cell lines.
      Maroschik, B; Gürtler, A; Krämer, A; Rößler, U; Gomolka, M; Hornhardt, S; Mörtl, S; Friedl, AA
      Radiation oncology (London, England)  9  15  2014

      Show Abstract
      Western Blotting24406105 24406105
      Certain and progressive methylation of histone H4 at lysine 20 during the cell cycle.
      Pesavento, James J, et al.
      Mol. Cell. Biol., 28: 468-86 (2008)  2008

      17967882 17967882
      A trans-tail histone code defined by monomethylated H4 Lys-20 and H3 Lys-9 demarcates distinct regions of silent chromatin.
      Sims, Jennifer K, et al.
      J. Biol. Chem., 281: 12760-6 (2006)  2006

      16517599 16517599

      Technical Info

      White Paper - The Message in the Marks: Deciphering Cancer Epigenetics (EMD)