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04-1479 Anti-methyl-PP2A Antibody, C subunit, clone 2A10

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04-1479
150 µg  
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      Overview

      Replacement Information
      Replacement Information04-1479 is a recommended replacement for 05-546.

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, Yeast (S. cerevisiae), R, Po, Rb, Ch, DrWBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number04-1479
      Replaces05-546
      DescriptionAnti-methyl-PP2A Antibody, C subunit, clone 2A10
      Background InformationType 2 protein serine/threonine phosphatase (PP2A) is a trimer composed of a catalytic C subunit and two regulatory (A and B) subunits; many isoforms for the A and B subunits have been found. PP2A has a high specific activity against the α subunit of phosphorylase kinase and is insensitive to Inhibitor-2. PP2A is distinguishable from PP2B/Calcineurin and PP2C by its partial activity in the absence of divalent cations, and by its high sensitivity to inhibition by okadaic acid. It is also thought to be the principal down-modulator of serine/threonine kinase cascades. PP2B requires Ca2+/calmodulin for activity, and is an activator of NFAT transcription factors. PP2C requires Mg2+, but is insensitive to okadaic acid, microcystin LR, or the calmodulin inhibitor trifluoperazine.
      References
      Product Information
      FormatPurified
      Control
      • Lysates from a yeast wild type strain (strain lacking the methyltransferase, delta PPM1) and a strain lacking the methylestrase, delta PPE1.
      PresentationPurified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl and 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-methyl-PP2A Antibody, C subunit, clone 2A10 is validated for use in WB for the detection of methyl-PP2A.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide (C-βA-RRTPDYFL-Ome) corresponding to amino acids 302-309 of human PP2A catalytic subunit. Clone 2A10.
      Clone2A10
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes the methylated form of PP2A, catalytic subunit.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      • Yeast (S. cerevisiae)
      • Rat
      • Pig
      • Rabbit
      • Chicken
      • Drosophila
      Species Reactivity NoteHuman, mouse and yeast. Predicted to cross react with rat, pig, rabbit, chicken and Drosophila.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Modifications
      • Methylation
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~36 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by western blot on lysates from a yeast wild type strain (strain lacking the methyltransferase, delta PPM1) and a strain lacking the methylestrase, delta PPE1.

      Western Blot Analysis: 1-5 µg/mL of this antibody detected the methylated form of PP2A catalytic subunit in yeast lysates lacking the methylestrase (increased methylation). Treatment of the blot with 0.2 M NaOH will demethylate proteins, thus eliminating detection of PP2A.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap.
      Packaging Information
      Material Size150 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-methyl-PP2A Antibody, C subunit, clone 2A10 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-methyl-PP2A Antibody, C subunit, clone 2A10 Certificates of Analysis

      TitleLot Number
      Anti-methyl-PP2A, C subunit, clone 2A10 - 2116004 2116004
      Anti-methyl-PP2A, C subunit, clone 2A10 - 2146011 2146011
      Anti-methyl-PP2A, C subunit, clone 2A10 - 2377651 2377651
      Anti-methyl-PP2A, C subunit, -2680521 2680521
      Anti-methyl-PP2A, C subunit, -2697391 2697391
      Anti-methyl-PP2A, C subunit, -2757106 2757106
      Anti-methyl-PP2A, C subunit, -2779130 2779130
      Anti-methyl-PP2A, C subunit, clone 2A10 - 1983712 1983712
      Anti-methyl-PP2A, C subunit, clone 2A10 - 2049815 2049815
      Anti-methyl-PP2A, C subunit, clone 2A10 - 2276407 2276407

      References

      Reference overviewApplicationPub Med ID
      Cytoplasmic SET induces tau hyperphosphorylation through a decrease of methylated phosphatase 2A.
      Chasseigneaux, S; Clamagirand, C; Huguet, L; Gorisse-Hussonnois, L; Rose, C; Allinquant, B
      BMC neuroscience  15  82  2014

      Show Abstract
      Western Blotting24981783 24981783
      Altered protein phosphatase 2A methylation and Tau phosphorylation in the young and aged brain of methylenetetrahydrofolate reductase (MTHFR) deficient mice.
      Sontag, JM; Wasek, B; Taleski, G; Smith, J; Arning, E; Sontag, E; Bottiglieri, T
      Frontiers in aging neuroscience  6  214  2014

      Show Abstract
      Western Blotting25202269 25202269
      NNMT promotes epigenetic remodeling in cancer by creating a metabolic methylation sink.
      Ulanovskaya, OA; Zuhl, AM; Cravatt, BF
      Nature chemical biology  9  300-6  2013

      Show Abstract
      Western Blotting23455543 23455543
      Protein phosphatase 2A carboxymethylation and regulatory B subunits differentially regulate mast cell degranulation.
      Kranias, Gregory, et al.
      Cell. Signal., 22: 1882-90 (2010)  2010

      Show Abstract
      20688157 20688157

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies