Millipore Sigma Vibrant Logo

ABN26 Anti-iNOS/NOS II Antibody, NT

View Products on Sigmaaldrich.com
ABN26
100 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, HWB, IP, IH(P)RbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABN26
      Replaces06-573
      DescriptionAnti-iNOS/NOS II Antibody, NT
      Alternate Names
      • Nitric oxide synthase, inducible
      • Inducible NO synthase
      • Inducible NOS
      • iNOS
      • Macrophage NOS
      • MAC-NOS
      • NOS type II
      • Peptidyl-cysteine S-nitrosylase NOS2
      Background InformationNitric oxide synthase, inducible (UniProt: P29477; also known as EC:1.14.13.39, Inducible NO synthase, Inducible NOS, iNOS, Macrophage NOS, MAC-NOS, NOS type II, Peptidyl-cysteine S-nitrosylase NOS2) is encoded by the Nos2 (also known as Inosl) gene (Gene ID: 18126) in murine species. Nitric oxide synthases (nNOS, iNOS, eNOS) are single polypeptides that differ in their rates of Nitric oxide ( NO) synthesis, Ca2+- dependence, cytochrome c reduction, and NADPH utilization. For their catalytic activities NOS isoforms require three distinct domains: (a) a reductase domain, (b) a calmodulin-binding domain, and (c) an oxygenase domain. The reductase domain contains the FAD and FMN moieties. The oxygenase domain, which contains the binding sites for heme, tetrahydrobiopterin, and arginine, catalyzes the conversion of L-arginine to citrulline and NO. The maximal rate of NO synthesis is established by the intrinsic maximum ability of the reductase domain to deliver electrons to the heme domain. The iNOS is expressed in a variety of cells and is localized mainly in the cytoplasm. It binds tightly to calmodulin at very low Ca2+ levels, hence the enzyme activity is unaffected by changes in intracellular Ca2+ levels. The complete Ca2+-independence of inducible NOS requires interactions of calmodulin with both the calmodulin-binding motif and the flavoprotein domain. This property is important in assuring a long-lasting release of NO. The iNOS is capable of producing large amounts of NO when induced by mediators such as TNF- , IL-1, IL-2, IL-6, and -IFN. The amount of NO released by iNOS falls in the nanomolar range. The iNOS-induced release of NO plays an important role in the macrophage-mediated response to infections, where NO acts as an effector molecule to kill the invading organism. This toxic effect is achieved via the production of highly reactive nitrogen and oxygen intermediates. The induction of iNOS has been implicated in increased blood flow to the tumor site resulting in tumor growth. The cytotoxic effects of NO have also been reported against cancer cells. Over-expression of iNOS has been implicated in a number of pathologies including septic shock where excessive generation of NO, in response to infection, leads to a serious drop in blood pressure and subsequent dysfunction of multiple organs.
      References
      Product Information
      FormatAffinity Purified
      Control
      • IFNgamma/LPS untreated and treated RAW264.7 cell lysates
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-iNOS/NOS II, NT, Cat. No. ABN26, is a rabbit polyclonal antibody that detects inducible nitric oxide synthase (iNOS) and is tested for use in Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunohistochemistry (Paraffin)
      Application NotesImmunoprecipitation Analysis: 10 µg from a representative lot detected iNOS/NOS II, NT in RAW 234.7 treated with INFgamma/LPS.

      Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected iNOS/NOS II, NT in human lung cancer and human colon cancer tissue sections.

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenGST-tagged recombinant fragment corresponding to the first 130 amino acids from the N-terminal region of murine inducible nitric oxide synthase (iNOS).
      EpitopeN-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects inducible nitric oxide synthase (iNOS). It targets an epitope within the N-terminal region.
      Species Reactivity
      • Mouse
      • Human
      Species Reactivity NoteHuman, Mouse. Predicted to react with Rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Nos2
      • Inosl
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.

      CATALYTIC ACTIVITY: L-arginine + n NADPH + n H+ + m O2 = citrulline + nitric oxide + n NADP+.

      COFACTOR: Heme group.

      Binds 1 FAD.

      Binds 1 FMN.

      Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.

      ENZYME REGULATION: Not stimulated by calcium/calmodulin. Aspirin inhibits expression and function of this enzyme and effects may be exerted at the level of translational/post-translational modification and directly on the catalytic activity.

      SUBUNIT STRUCTURE: Homodimer. Binds SLC9A3R1 (By similarity).

      TISSUE SPECIFICTY: Macrophages.

      INDUCTION: By treatment with endotoxins or cytokines.

      SEQUENCE SIMILARITIES: Belongs to the NOS family.

      Contains 1 FAD-binding FR-type domain.

      Contains 1 flavodoxin-like domain.
      Molecular Weight~130 kDa observed; 135.58 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in RAW 234.7 treated with INFgamma/LPS cell lysate.

      Western Blotting Analysis: A 1:2,000 dilution of this antibody detected iNOS/NOS II, NT in RAW 234.7 treated with INFgamma/LPS cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-iNOS/NOS II Antibody, NT SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-iNOS/NOS II Antibody, NT Certificates of Analysis

      TitleLot Number
      Anti-iNOS/NOS II, NT 2445053
      Anti-iNOS/NOS II, NT (rabbit polyclonal) Polyclonal Antibody Q2922275
      Anti-iNOS/NOS II, NT - 2118972 2118972
      Anti-iNOS/NOS II, NT - 2153050 2153050
      Anti-iNOS/NOS II, NT - 2377298 2377298
      Anti-iNOS/NOS II, NT - 2424798 2424798
      Anti-iNOS/NOS II, NT - 2224493 2224493
      Anti-iNOS/NOS II, NT - 2281700 2281700
      Anti-iNOS/NOS II, NT - 2982380 2982380
      Anti-iNOS/NOS II, NT - 3182613 3182613

      References

      Reference overviewApplicationPub Med ID
      Crosstalk between glioma-initiating cells and endothelial cells drives tumor progression.
      Jeon, HM; Kim, SH; Jin, X; Park, JB; Kim, SH; Joshi, K; Nakano, I; Kim, H
      Cancer research  74  4482-92  2014

      Show Abstract
      Immunofluorescence24962027 24962027
      SMAD3 deficiency promotes inflammatory aortic aneurysms in angiotensin II-infused mice via activation of iNOS.
      Tan, CK; Tan, EH; Luo, B; Huang, CL; Loo, JS; Choong, C; Tan, NS
      Journal of the American Heart Association  2  e000269  2013

      Show Abstract
      23782924 23782924

      Brochure

      Title
      Pathways and Biomarkers of Oxidative Stress (EMD)

      Technical Info

      Title
      White Paper- Modern Methods in Oxidative Stress Research (EMD)