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07-213 Anti-dimethyl-Histone H4 (Arg3) Antibody

100 µL  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Vrt, Inv WB Rb Affinity Purified Polyclonal Antibody
      Catalogue Number07-213
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-dimethyl-Histone H4 (Arg3) Antibody
      Alternate Names
      • H4 histone family, member A
      • histone 1
      • H4a
      • histone cluster 1
      • H4a
      Background InformationHistone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).
      Product Information
      FormatAffinity Purified
      • Acid extracted proteins from HeLa cells.
      PresentationPurified rabbit polyclonal IgG in buffer containing 70% storage buffer 0.2 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide, 5 mg/mL BSA and 30% glycerol.
      ApplicationAnti-dimethyl-Histone H4 (Arg3) Antibody is a Rabbit Polyclonal Antibody for detection of dimethyl-Histone H4 (Arg3) also known as H4 histone family member A, histone 1 H4a & has been validated in WB.
      Key Applications
      • Western Blotting
      Application NotesMethylation/Western Blot Assay: Reported by an independent laboratory to detect Histone H4 methylated in vitro by recombinant PRMT1 and detected by western blot analysis.
      Biological Information
      ImmunogenBSA-conjugated synthetic peptide (SG[me2R]GKGGKG-C) corresponding to amino acids 1-9 of Histone H4 with a C-terminal cysteine added to facilitate conjugation. The immunizing sequence is conserved from Tetrahymena to human.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityRecognizes methylated Histone H4 (Arg3), MW 10 kDa. Addition of 0.1% Tween®-20 in western blot buffers results in the detection of additional proteins at 17 kDa (possibly methylated Histone H2a), 34 kDa and 80 kDa. Specificity demonstrated by ELISA using methylated and unmethylated Histone H4 peptides. The antibody does not detect recombinant Histone H3 purified from E. coli.
      Species Reactivity
      • Human
      • Vertebrates
      • Invertebrates
      Species Reactivity NoteHuman. Broad species cross-reactivity is expected.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element.
      Gene Symbol
      • HIST1H4A
      • H4/J
      • HIST1H4F
      • HIST1H4L
      • H4FN
      • H4FA
      • H4FH
      • HIST1H4H
      • H4F2
      • H4FM
      • H4/A
      • H4FK
      • H4FG
      • HIST2H4
      • H4FB
      • H4/K
      • HIST1H4I
      • H4/N
      • HIST1H4B
      • H4FE
      • H4/M
      • H4/a
      • HIST1H4E
      • HIST4H4
      • HIST2H4A
      • H4FC
      • H4FI
      • H4/H
      • HIST1H4C
      • H4/G
      • HIST1H4K
      • H4FJ
      • H4FD
      • H4/I
      • H4/B
      • H4/D
      • H4/C
      • HIST1H4J
      • HIST1H4D
      • H4/E
      • Methylation
      Purification MethodImmunoaffinity Chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P62805 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 103 amino acids; 11367 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      PTM: Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage (By similarity). & Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.
      SIMILARITY: SwissProt: P62805 ## Belongs to the histone H4 family.
      Molecular Weight10 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceWestern Blot Analysis: A 1:500-1:2000 dilution of this antibody detected methylated Histone H4 in acid-extracted proteins from HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information


      Anti-dimethyl-Histone H4 (Arg3) Antibody Certificates of Analysis

      TitleLot Number
      Anti-dimethyl-Histone H4 (Arg3) - 2045020450
      Anti-dimethyl-Histone H4 (Arg3) - 2277922779
      Anti-dimethyl-Histone H4 (Arg3) - 2297422974
      Anti-dimethyl-Histone H4 (Arg3) - 2400424004
      Anti-dimethyl-Histone H4 (Arg3) - 2773527735
      Anti-dimethyl-Histone H4 (Arg3) - 2994229942


      Reference overviewApplicationSpeciesPub Med ID
      The HPV E6 oncoprotein targets histone methyltransferases for modulating specific gene transcription.
      Hsu, CH; Peng, KL; Jhang, HC; Lin, CH; Wu, SY; Chiang, CM; Lee, SC; Yu, WC; Juan, LJ
      Oncogene  31  2335-49  2012

      Show Abstract
      Western Blotting21963854 21963854
      Acute running stimulates hippocampal dopaminergic neurotransmission in rats, but has no influence on brain-derived neurotrophic factor.
      Maaike Goekint,Inge Bos,Elsa Heyman,Romain Meeusen,Yvette Michotte,Sophie Sarre
      Journal of applied physiology (Bethesda, Md. : 1985)  112  2012

      Show Abstract
      22134693 22134693
      Characterization of the PRMT gene family in rice reveals conservation of arginine methylation.
      Ahmad, A; Dong, Y; Cao, X
      PloS one  6  e22664  2011

      Show Abstract Full Text Article
      Western Blotting21853042 21853042
      Analysis of histones in Xenopus laevis. I. A distinct index of enriched variants and modifications exists in each cell type and is remodeled during developmental transitions.
      Shechter, D; Nicklay, JJ; Chitta, RK; Shabanowitz, J; Hunt, DF; Allis, CD
      The Journal of biological chemistry  284  1064-74  2009

      Show Abstract
      18957438 18957438
      Functional connection between deimination and deacetylation of histones.
      Denis, H; Deplus, R; Putmans, P; Yamada, M; Métivier, R; Fuks, F
      Molecular and cellular biology  29  4982-93  2009

      Show Abstract
      19581286 19581286
      The protein arginine methyltransferases CARM1 and PRMT1 cooperate in gene regulation.
      Kleinschmidt, MA; Streubel, G; Samans, B; Krause, M; Bauer, UM
      Nucleic acids research  36  3202-13  2008

      Show Abstract Full Text Article
      18413343 18413343
      PRMT6-mediated methylation of R2 in histone H3 antagonizes H3 K4 trimethylation.
      Hyllus, D; Stein, C; Schnabel, K; Schiltz, E; Imhof, A; Dou, Y; Hsieh, J; Bauer, UM
      Genes & development  21  3369-80  2007

      Show Abstract Full Text Article
      Human18079182 18079182
      hCAF1, a new regulator of PRMT1-dependent arginine methylation.
      Robin-Lespinasse, Y; Sentis, S; Kolytcheff, C; Rostan, MC; Corbo, L; Le Romancer, M
      Journal of cell science  120  638-47  2007

      Show Abstract
      17264152 17264152
      USF1 recruits histone modification complexes and is critical for maintenance of a chromatin barrier.
      Huang, S; Li, X; Yusufzai, TM; Qiu, Y; Felsenfeld, G
      Molecular and cellular biology  27  7991-8002  2007

      Show Abstract Full Text Article
      17846119 17846119
      Coactivator-associated arginine methyltransferase 1 (CARM1) is a positive regulator of the Cyclin E1 gene.
      El Messaoudi, S; Fabbrizio, E; Rodriguez, C; Chuchana, P; Fauquier, L; Cheng, D; Theillet, C; Vandel, L; Bedford, MT; Sardet, C
      Proceedings of the National Academy of Sciences of the United States of America  103  13351-6  2006

      Show Abstract
      16938873 16938873


      Is this peptide asymmetric or symmetric?This is an asymmetric dimethyl-modified Histone H4 peptide.