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ABE323 Anti-dimethyl Histone H4 Antibody (Arg17)

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100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, DB, ICCRbAffinity PurifiedPolyclonal Antibody
      Catalogue NumberABE323
      DescriptionAnti-dimethyl Histone H4 Antibody (Arg17)
      Alternate Names
      • Histone H4
      Background InformationHistone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).
      Product Information
      FormatAffinity Purified
      • Untreated and CARM1 treated Histone H4 recombinant proteins.
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      ApplicationDetect Histone H4 using this rabbit polyclonal antibody, Anti-dimethyl Histone H4 Antibody (Arg17) validated for use in western blotting, Dot Blot & ICC.
      Key Applications
      • Western Blotting
      • Dot Blot
      • Immunocytochemistry
      Application NotesDot Blot (Specificity) Analysis: A 1:200 dilution from a representative lot detected asymmetric Histone H4 dimethylated at Arg17, but not unmethylated Histone H4 or other peptides corresponding to modified histones.

      Immunocytochemistry Analysis: A 1:2,000 dilution from a representative lot detected Histone H4 dimethylated at Arg17 in MEF-1 cells.

      Alexa Fluor® is a registered trademark of Life Technologies®.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the N-terminus of human asymmetric Histone H4 dimethylated at Arg17.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody recognizes the N-terminus of asymmetric Histone H4 dimethylated at Arg17.
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HIST1H4A
      • H4/A
      • H4FA
      • HIST1H4B
      • H4/I
      • H4FI
      • HIST1H4C
      • H4/G
      • H4FG
      • HIST1H4D
      • H4/B
      • H4FB
      • HIST1H4E
      • H4/J
      • H4FJ
      • HIST1H4F
      • H4/C
      • H4FC
      • HIST1H4H
      • H4/H
      • H4FH
      • HIST1H4I
      • H4/M
      • H4FM
      • HIST1H4J
      • H4/E
      • H4FE
      • HIST1H4K
      • H4/D
      • H4FD
      • HIST1H4L
      • H4/K
      • H4FK
      • HIST2H4A
      • H4/N
      • H4F2
      • H4FN
      • HIST2H4
      • HIST2H4B
      • H4/O
      • H4FO
      • HIST4H4
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~13 kDa observed
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in untreated and CARM1 treated Histone H4 recombinant proteins.

      Western Blotting Analysis: 0.25 µg/mL of this antibody detected asymmetric Histone H4 dimethylated at Arg17 in CARM1 treated Histone H4 recombinant proteins.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information