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07-030 Anti-dimethyl-Histone H3 (Lys4) Antibody

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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, TDB, ICC, IF, WB, PIA, ChIP-seq, ChIPRbUnpurifiedPolyclonal Antibody
      Catalogue Number07-030
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-dimethyl-Histone H3 (Lys4) Antibody
      Alternate Names
      • H3K4me2
      • Histone H3 (di methyl K4)
      • H3 histone family, member T
      • histone 3
      • H3
      • histone cluster 3
      • H3
      Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
      Product Information
      • HeLa acid extract
      PresentationRabbit polyclonal antiserum containing 0.05% sodium azide.
      Quality LevelMQ100
      ApplicationUse Anti-dimethyl-Histone H3 (Lys4) Antibody (rabbit polyclonal antibody) is published and validated in ChIP, DB, ICC, IF, WB, PIA, ChIP-seq. to detect dimethyl-Histone H3 (Lys4) also known as H3K4me2, Histone H3 (di methyl K4).
      Key Applications
      • Dot Blot
      • Immunocytochemistry
      • Immunofluorescence
      • Western Blotting
      • Peptide Inhibition Assay
      • ChIP-seq
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesChromatin Immunoprecipitation:
      5 μL of a previous lot immunoprecipitated methylated Histone H3 from 1 x 106 3T3/A31 cells.

      1:500 dilution of a previous lot showed positive immunostaining for methylated Histone H3 in L6 cells fixed with 4% paraformaldehyde and permeabilized using 0.1% Triton X-100.
      Biological Information
      ImmunogenKLH conjugated synthetic peptide (ARTMe2KQTAR-GC) corresponding to amino acids 1-8 of human Histone H3.
      Epitopedimethylated Histone H3 (Lys4)
      SpecificityRecognizes dimethylated Histone H3 (Lys4), MW ~17 kDa. Specificity demonstrated by ELISA using methylated peptides.
      Species Reactivity
      • Human
      • Tetrahymena
      Species Reactivity NoteHuman and tetrahymena. Broad species cross-reactivity is expected.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
      Gene Symbol
      • HIST3H3
      • H3FT
      • H3/t
      • H3t
      • MGC126888
      • H3/g
      • H3.4
      • H3T
      • MGC126886
      • Methylation
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 136 amino acids; 15508 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      TISSUE SPECIFICITY: Expressed in testicular cells.
      DEVELOPMENTAL STAGE: Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
      PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity).
      SIMILARITY: SwissProt: Q16695 ## Belongs to the histone H3 family.
      Molecular WeightApprox. 17 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by western blot on HeLa acid extract.

      Western Blot Analysis: 1:2000-1:10,000 dilution of this antibody detected methylated Histone H3 in acid extracted proteins from HeLa cells.
      1:2000-1:5000 dilution of a previous lot detected methylated Histone H3 in acid extracted proteins from HeLa and tetrahymena cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Global Trade Item Number
      Catalog Number GTIN
      07-030 04053252269202


      Anti-dimethyl-Histone H3 (Lys4) Antibody SDS


      Safety Data Sheet (SDS) 

      Anti-dimethyl-Histone H3 (Lys4) Antibody Certificates of Analysis

      TitleLot Number
      Anti-dimethyl-Histone H3 (Lys4) 2477948
      Anti-dimethyl-Histone H3 (Lys4) (rabbit antiserum) - DAM1474881 DAM1474881
      Anti-dimethyl-Histone H3 (Lys4) - 2019745 2019745
      Anti-dimethyl-Histone H3 (Lys4) - 2142243 2142243
      Anti-dimethyl-Histone H3 (Lys4) - 2430486 2430486
      Anti-dimethyl-Histone H3 (Lys4) - 20132 20132
      Anti-dimethyl-Histone H3 (Lys4) - 2089140 2089140
      Anti-dimethyl-Histone H3 (Lys4) - 2225237 2225237
      Anti-dimethyl-Histone H3 (Lys4) - 22672 22672
      Anti-dimethyl-Histone H3 (Lys4) - 2309072 2309072


      Reference overviewApplicationSpeciesPub Med ID
      Lysine-specific demethylase (LSD1/KDM1A) and MYCN cooperatively repress tumor suppressor genes in neuroblastoma.
      Amente, S; Milazzo, G; Sorrentino, MC; Ambrosio, S; Di Palo, G; Lania, L; Perini, G; Majello, B
      Oncotarget  6  14572-83  2015

      Show Abstract
      26062444 26062444
      Poised chromatin and bivalent domains facilitate the mitosis-to-meiosis transition in the male germline.
      Sin, HS; Kartashov, AV; Hasegawa, K; Barski, A; Namekawa, SH
      BMC biology  13  53  2015

      Show Abstract
      26198001 26198001
      RE-IIBP Methylates H3K79 and Induces MEIS1-mediated Apoptosis via H2BK120 Ubiquitination by RNF20.
      Woo Park, J; Kim, KB; Kim, JY; Chae, YC; Jeong, OS; Seo, SB
      Scientific reports  5  12485  2015

      Show Abstract
      26206755 26206755
      Chromatin organization and cytological features of carnivorous Genlisea species with large genome size differences.
      Tran, TD; Cao, HX; Jovtchev, G; Novák, P; Vu, GT; Macas, J; Schubert, I; Fuchs, J
      Frontiers in plant science  6  613  2015

      Show Abstract
      26347752 26347752
      Epigenetic synergy between decitabine and platinum derivatives.
      Qin, T; Si, J; Raynal, NJ; Wang, X; Gharibyan, V; Ahmed, S; Hu, X; Jin, C; Lu, Y; Shu, J; Estecio, MR; Jelinek, J; Issa, JP
      Clinical epigenetics  7  97  2015

      Show Abstract
      26366234 26366234
      A comprehensive epigenome map of Plasmodium falciparum reveals unique mechanisms of transcriptional regulation and identifies H3K36me2 as a global mark of gene suppression.
      Karmodiya, K; Pradhan, SJ; Joshi, B; Jangid, R; Reddy, PC; Galande, S
      Epigenetics & chromatin  8  32  2015

      Show Abstract
      26388940 26388940
      The Nucleosome Acidic Patch Regulates the H2B K123 Monoubiquitylation Cascade and Transcription Elongation in Saccharomyces cerevisiae.
      Cucinotta, CE; Young, AN; Klucevsek, KM; Arndt, KM
      PLoS genetics  11  e1005420  2015

      Show Abstract
      26241481 26241481
      Histone modifications rather than the novel regional centromeres of Zymoseptoria tritici distinguish core and accessory chromosomes.
      Schotanus, K; Soyer, JL; Connolly, LR; Grandaubert, J; Happel, P; Smith, KM; Freitag, M; Stukenbrock, EH
      Epigenetics & chromatin  8  41  2015

      Show Abstract
      26430472 26430472
      An integrative analysis of post-translational histone modifications in the marine diatom Phaeodactylum tricornutum.
      Veluchamy, A; Rastogi, A; Lin, X; Lombard, B; Murik, O; Thomas, Y; Dingli, F; Rivarola, M; Ott, S; Liu, X; Sun, Y; Rabinowicz, PD; McCarthy, J; Allen, AE; Loew, D; Bowler, C; Tirichine, L
      Genome biology  16  102  2015

      Show Abstract
      25990474 25990474
      An Lnc RNA (GAS5)/SnoRNA-derived piRNA induces activation of TRAIL gene by site-specifically recruiting MLL/COMPASS-like complexes.
      He, X; Chen, X; Zhang, X; Duan, X; Pan, T; Hu, Q; Zhang, Y; Zhong, F; Liu, J; Zhang, H; Luo, J; Wu, K; Peng, G; Luo, H; Zhang, L; Li, X; Zhang, H
      Nucleic Acids Res  43  3712-25  2015

      Show Abstract
      25779046 25779046

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