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PC05 Anti-c-Fos (Ab-2) (4-17) Rabbit pAb

Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H, M, R Rb Polyclonal Antibody

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PC05-100UG
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      Description
      OverviewRecognizes the ~50-62 kDa c-Fos protein in HepG2, NIH3T3, and MCF-7 cells.
      Catalogue NumberPC05
      Brand Family Calbiochem®
      References
      ReferencesDeTogni, P., et al. 1988. Cell Biol. 8, 2251.
      Rouscher III, F.J., et al. 1988. Science 240, 1010.
      Sassone-Corsi, P., et al. 1988. Cell 54, 553.
      Verma, I.M. and Graham, W.R. 1987. Cancer Res. 49, 29.
      Verma, I.M. and Sassone-Corsi, P. 1987. Cell 51, 513.
      Muller, R. 1986. Biochim. Biophys. Acta 823, 207.
      Verma, I. 1986. Trends Genet. 2, 93.
      Greenberg, M. and Ziff, E. 1984. Nature (London), 311, 433.
      Product Information
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
      Positive controlHepG2, NIH 3T3 cells, MCF-7 cells, colon or breast adenocarcinoma tissue
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoprecipitation
      Not Free-floating Sections
      Paraffin Sections
      Frozen Sections
      Immunoblotting (Western Blotting)
      Immunofluorescence
      Application NotesFrozen sections (5-10 µg/ml)
      Immunoblotting (1-5 µg/ml)
      Immunofluorescence (1-5 µg/ml)
      Immunoprecipitation (1-2 µg/reaction)
      Paraffin sections (5-10 µg/ml, pepsin or heat pre-treatment required)
      Free-floating Sections (not recommended)
      Application CommentsFor gel shift assays, use Cat. No. PC05L resuspended in 100 µl of buffer. The immunogen, c-fos (Peptide-2), Cat. No. PP10, is also available for competition studies (see Murphy, et al. below). Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide (SGFNADYEASSSRC) (Cat. No. PP10) corresponding to amino acids 4-17 of human c-Fos
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Highly Toxic
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-c-Fos (Ab-2) (4-17) Rabbit pAb Certificates of Analysis

      TitleLot Number
      PC05

      References

      Reference overview
      DeTogni, P., et al. 1988. Cell Biol. 8, 2251.
      Rouscher III, F.J., et al. 1988. Science 240, 1010.
      Sassone-Corsi, P., et al. 1988. Cell 54, 553.
      Verma, I.M. and Graham, W.R. 1987. Cancer Res. 49, 29.
      Verma, I.M. and Sassone-Corsi, P. 1987. Cell 51, 513.
      Muller, R. 1986. Biochim. Biophys. Acta 823, 207.
      Verma, I. 1986. Trends Genet. 2, 93.
      Greenberg, M. and Ziff, E. 1984. Nature (London), 311, 433.

      Citations

      Title
    • Abigail Derbyshire and Mike Ludwig. (2004) TFF3 induced Fos protein expression in the magnocellular oxytocin neurons of the hypothalamus. Peptides 25, 833-838.
    • Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision17-September-2015 JSW
      ApplicationFrozen sections (5-10 µg/ml)
      Immunoblotting (1-5 µg/ml)
      Immunofluorescence (1-5 µg/ml)
      Immunoprecipitation (1-2 µg/reaction)
      Paraffin sections (5-10 µg/ml, pepsin or heat pre-treatment required)
      Free-floating Sections (not recommended)
      DescriptionProtein A purified rabbit polyclonal antibody. Recognizes v-fos and ~50-62 kDa c-fos proteins.
      BackgroundThe proto-oncogene fos has been implicated in cell growth, differentiation and development. Fos is induced by a large number of stimuli including mitogens, differentiation-specific agents, pharmacological agents, etc. Induction of the 62,000 dalton fos protein is rapid but transient. The fos protein is associated with a 39,000 dalton protein which has been identified as the protein product of the jun proto-oncogene (c-jun). The fos/jun protein complex binds specifically to a sequence element in DNA referred to as the AP-1 binding site.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide (SGFNADYEASSSRC) (Cat. No. PP10) corresponding to amino acids 4-17 of human c-Fos
      IsotypeIgG
      Specieshuman, mouse, rat
      Positive controlHepG2, NIH 3T3 cells, MCF-7 cells, colon or breast adenocarcinoma tissue
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsFor gel shift assays, use Cat. No. PC05L resuspended in 100 µl of buffer. The immunogen, c-fos (Peptide-2), Cat. No. PP10, is also available for competition studies (see Murphy, et al. below). Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Highly Toxic
      ReferencesDeTogni, P., et al. 1988. Cell Biol. 8, 2251.
      Rouscher III, F.J., et al. 1988. Science 240, 1010.
      Sassone-Corsi, P., et al. 1988. Cell 54, 553.
      Verma, I.M. and Graham, W.R. 1987. Cancer Res. 49, 29.
      Verma, I.M. and Sassone-Corsi, P. 1987. Cell 51, 513.
      Muller, R. 1986. Biochim. Biophys. Acta 823, 207.
      Verma, I. 1986. Trends Genet. 2, 93.
      Greenberg, M. and Ziff, E. 1984. Nature (London), 311, 433.
      Citation
    • Abigail Derbyshire and Mike Ludwig. (2004) TFF3 induced Fos protein expression in the magnocellular oxytocin neurons of the hypothalamus. Peptides 25, 833-838.
    • Related Products & Applications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies