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OP67 Anti-c-ErbB2/c-Neu (Ab-6) Mouse mAb (OD-3)

OP67
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H M Monoclonal Antibody

Pricing & Availability

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OP67-100UG
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      Plastic ampoule 100 μg
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      Description
      OverviewRecognizes the ~97-115 kDa truncated form of c-ErbB2/c-Neu in human serum as well as the ~185 kDa full-length protein. Does not cross-react with the EGF receptor.
      Catalogue NumberOP67
      Brand Family Calbiochem®
      SynonymsAnti-HER2, Anti-Neu, Anti-ErbB2, Anti-Erythroblastosis Virus, Anti-Human Epidermal Growth Factor
      References
      ReferencesCarney, W.P, et al. 1991. J. Tumor Marker Oncol. 6, 53.
      DiFiore, P.P., et al. 1987. Science 237, 178.
      Slamon, D.J., et al. 1987. Science 235, 177.
      Varley, J.M., et al. 1987. Oncogene 1, 423.
      Yamamoto, T., et al. 1986. Nature 319, 230.
      Blick, M., et al. 1984. Blood 64, 1234.
      Schwab, M., et al. 1984. Cold Spring Harbor Laboratory 2, 215.
      Bargmann, C.I., et al. 1968. Nature 319, 226.
      Product Information
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
      Negative controlHT 1080 or HepG2 cells
      Positive controlSK-BR-3 cell conditioned medium
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesImmunoblotting Carney, W.P, et al. 1991. J. Tumor Marker Oncol. 6, 53.
      Key Applications Not Immunoprecipitation
      Not Paraffin Sections
      Immunoblotting (Western Blotting)
      Not Frozen Sections
      Not Immunofluorescence
      Application NotesImmunoblotting (10 µg/ml)
      Frozen Sections (not recommended)
      Paraffin Sections (not recommended)
      Immunofluorescence (not recommended)
      Immunoprecipitation (not recommended)
      Application CommentsThis antibody reacts with an extracellular epitope and does not cross react with human EGF-receptor. For best results, use IgM-specific secondary reagents. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogencells overexpressing human c-Neu
      ImmunogenHuman
      CloneOD-3
      HostMouse
      IsotypeIgM
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-c-ErbB2/c-Neu (Ab-6) Mouse mAb (OD-3) Certificates of Analysis

      TitleLot Number
      OP67

      References

      Reference overview
      Carney, W.P, et al. 1991. J. Tumor Marker Oncol. 6, 53.
      DiFiore, P.P., et al. 1987. Science 237, 178.
      Slamon, D.J., et al. 1987. Science 235, 177.
      Varley, J.M., et al. 1987. Oncogene 1, 423.
      Yamamoto, T., et al. 1986. Nature 319, 230.
      Blick, M., et al. 1984. Blood 64, 1234.
      Schwab, M., et al. 1984. Cold Spring Harbor Laboratory 2, 215.
      Bargmann, C.I., et al. 1968. Nature 319, 226.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision03-October-2007 RFH
      SynonymsAnti-HER2, Anti-Neu, Anti-ErbB2, Anti-Erythroblastosis Virus, Anti-Human Epidermal Growth Factor
      ApplicationImmunoblotting (10 µg/ml)
      Frozen Sections (not recommended)
      Paraffin Sections (not recommended)
      Immunofluorescence (not recommended)
      Immunoprecipitation (not recommended)
      DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing with SP2/0 mouse myeloma cells. Recognizes the ~185 kDa full-length and the ~97-115 kDa truncated forms of c-ErbB2/c-Neu.
      BackgroundAmplification of the human proto-oncogene c-neu occurs in approximately 30% of breast carcinomas and the amplification maybe a useful predictor of disease prognosis. Gene amplification and the resulting overexpression of proto-oncogene encoded proteins is thought to transform cells by chronically stimulating signal transduction pathways and in model systems overexpression of human c-neu induces cellular transformation. In the rat model system, a point mutation has been located within the transmembrane domain of the c-neu oncogene. neu (from neuroglioblastomas) is also referred to as erbB-2 (from erythroblastosis virus) and HER2 (for Human Epidermal Growth Factor Receptor) and was the second of four members of the EGF receptor family to be described.
      HostMouse
      Immunogen speciesHuman
      Immunogencells overexpressing human c-Neu
      CloneOD-3
      IsotypeIgM
      Specieshuman, not mouse, not rat
      Positive controlSK-BR-3 cell conditioned medium
      Negative controlHT 1080 or HepG2 cells
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsThis antibody reacts with an extracellular epitope and does not cross react with human EGF-receptor. For best results, use IgM-specific secondary reagents. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesCarney, W.P, et al. 1991. J. Tumor Marker Oncol. 6, 53.
      DiFiore, P.P., et al. 1987. Science 237, 178.
      Slamon, D.J., et al. 1987. Science 235, 177.
      Varley, J.M., et al. 1987. Oncogene 1, 423.
      Yamamoto, T., et al. 1986. Nature 319, 230.
      Blick, M., et al. 1984. Blood 64, 1234.
      Schwab, M., et al. 1984. Cold Spring Harbor Laboratory 2, 215.
      Bargmann, C.I., et al. 1968. Nature 319, 226.
      Application referencesImmunoblotting Carney, W.P, et al. 1991. J. Tumor Marker Oncol. 6, 53.