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MABF2094-100UG Anti-alpha TLR10 Antibody, clone 5C2C5

MABF2094-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF2094-100UG
      DescriptionAnti-alpha TLR10 Antibody, clone 5C2C5
      Alternate Names
      • CD290
      Background InformationToll-like receptor 10 (UniProt: Q9BXR5; also known as CD290) is encoded by the TLR10 (also known as UNQ315/PRO358) gene (Gene ID: 81793) in human. Toll-like Receptors (TLRs) are transmembrane proteins that are expressed on various immune cells. The extracellular N-terminal region of TLRs recognizes specific pathogen components. At least 10 different members of TLR family have been identified in humans that detect different pathogen associated molecular patterns (PAMPs), including lipopolysaccharides, flagellin, bacterial CpG DNA, and viral RNA and DNA. Recognition of PAMPs by TLRs is considered as a key process for the induction of an inflammatory response. TLR10 is a single-pass type I membrane protein that is highly expressed in spleen, lymph node, thymus, and tonsil. Higher expressions have also been reported in promyelocytic HL-60 and in B-cell lines. It acts via MYD88 and TRAF6, leading to cytokine secretion and the inflammatory response. In TLR10-expressing cells, IkappaB alpha is shown to be consistently inhibited suggesting that TLR10 suppresses NF-kB signaling upstream of IkappaB alpha degradation. TLR10 is synthesized with a signal peptide (aa 1-19) that is cleaved off in the mature form, which contains an extracellular domain (aa 20-576), a transmembrane domain (aa 577-597), and a cytoplasmic domain (aa 598-811). Clone 5C2C5 is shown to suppress LPS-induced IL-6 production in isolated monocytes. (Ref: Hess, NJ., et al. (2017). J. Leukoc. Biol. 101(5); 1245-1252; Jiang, S., et al. (2016). J. Immunol. 196(9): 3834-3841).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in PBS without azide.
      Applications
      ApplicationAnti-alpha TLR10, clone 5C2C5, Cat. No. MABF2094, is a mouse monoclonal antibody that detects Toll-like receptor 10 and has been tested for use in Flow Cytometry and Inhibition/Functional Analysis.
      Key Applications
      • Flow Cytometry
      • Inhibits Activity/Function
      Application NotesAgonist Analysis: A representative lot activated TLR10 receptor when added 30 minutes prior to stiimulation of monocytes. (Hess, N.J., et. al. (2017). J Leukoc Biol. 101(5):1245-1252).

      Flow Cytometry Analysis: A representative lot detected alpha TLR10 in Flow Cytometry applications (Hess, N.J., et. al. (2017). J Leukoc Biol. 101(5):1245-1252; Hess, N.J., et. al. (2017). J Immunol. 198(2):699-707).

      Inhibition/Function Analysis: A representative lot suppressed LPS-induced IL-6 production in isolated monocytes when added after stimulation. (Hess, N.J., et. al. (2017). J Leukoc Biol. 101(5):1245-1252).
      Biological Information
      ImmunogenRecombinant fragment corresponding to 455 amino acids from the N-terminal, extracellular domain of human Toll-like receptor 10.
      Epitopeextracellular domain
      Clone5C2C5
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 5C2C5 is a mouse monoclonal antibody that detects human Toll-like receptor 10.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TLR10
      • UNQ315/PRO358
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight94.56 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in human peripheral blood mononuclear cells (PBMC).

      Flow Cytometry Analysis: 2 µg/mL of this antibody detected alpha TLR10 in one million human peripheral blood mononuclear cells (PBMC).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-alpha TLR10 Antibody, clone 5C2C5 Certificates of Analysis

      TitleLot Number
      Anti-alpha TLR10, clone 5C2C5 - Q3245721Q3245721

      References

      Reference overviewPub Med ID
      TLR10 Is a B Cell Intrinsic Suppressor of Adaptive Immune Responses.
      Hess, NJ; Jiang, S; Li, X; Guan, Y; Tapping, RI
      J Immunol  198  699-707  2017

      Show Abstract
      27956526 27956526
      TLR10 suppresses the activation and differentiation of monocytes with effects on DC-mediated adaptive immune responses.
      Hess, NJ; Felicelli, C; Grage, J; Tapping, RI
      J Leukoc Biol  101  1245-1252  2017

      Show Abstract
      28235773 28235773