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ABE286 Anti-acetyl-p53 (Lys120) Antibody

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ABE286
200 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HDB, IPRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE286
      DescriptionAnti-acetyl-p53 (Lys120) Antibody
      Alternate Names
      • Cellular tumor antigen p53
      • Antigen NY-CO-13
      • Phosphoprotein p53
      • Tumor suppressor p53
      Background Informationp53 is a DNA-binding, oligomerization domain and transcription activation domain-containing tumor suppressor that upregulates growth arrest and apoptosis-related genes in response to stress signals, thereby influencing programmed cell death, cell differentiation and cell cycle control mechanisms. p53 localizes to the nucleus yet can be chaperoned to the cytoplasm by the negative regulator MDM2, an E3 ubiquitin ligase that is upregulated in the presence of active p53, where MDM2 polyubiquitinates p53 for proteasome targeting. p53 can assemble into tetramers in the absence of DNA, fluctuates between latent and active (DNA-binding) conformations, and is differentially activated through posttranslational modifications including phosphorylation and acetylation. Mutations in the DNA-binding domain (DBD) (amino acids 110-286) of p53 can compromise energetically favorable association with cis elements and are implicated in several human cancers.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Non-acetylated and acetylated p53 peptides
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-acetyl-p53 (Lys120) Antibody is a highly specific rabbit polyclonal antibody, that targets p53 & has been tested in Dot Blot & IP.
      Key Applications
      • Dot Blot
      • Immunoprecipitation
      Application NotesImmunoprecipitation Analysis: 1 µg from a representative lot immunoprecipitated acetyl-p53 in camptothecin treated HCT116 cell lysate.

      Immunoprecipitation Analysis: 1 µg from a representative lot immunoprecipitated acetyl-p53 in p53 transfected H1299 human lung cotransfected with or without hMOF (Prof. S. McMahon, Kimmel Cancer Center of Thomas Jefferson Medical College).

      Immunoprecipitation Analysis: 1.5 µg or 4.5 µg from a representative lot immunoprecipitated endogenous acetyl-p53 in HCT116 cell lysates, which were treated with a vehicle (DMSO) or camptothecin (CPT) (Prof. S. McMahon, Kimmel Cancer Center of Thomas Jefferson Medical College).

      Immunoprecipitation Analysis: 1 µg from a representative lot immunoprecipitated acetyl-p53 in H1299 cell lysates, which were transfected with either wild type p53, the 9 K/R mutant (all lysine residues except Lys120 mutated to arginine), the 10 K/R mutant (all lysine residues mutate including Lys 120), or empty vector, with or without hMOF (Prof. S. McMahon, Kimmel Cancer Center of Thomas Jefferson Medical College).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the AXIN1 interacting domain of human p53 acetylated at Lys120.
      EpitopeAXIN1 interacting domain
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes the AXIN1 interacting domain of p53 acetylated at Lys120.
      Species Reactivity
      • Human
      Species Reactivity NoteDemonstrated to react with Human. Predicted to react with Sheep, Porcine, Rabbit, and Canine based on 100% sequence homology. Other homologies: Mouse, Rat, Bovine, Feline, Zebrafish, and Equine (88% sequence homology).
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TP53
      • P53
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~43 kDa calculated. Many isoforms are known to exist. This antibody is predicted to react with isoform 1 (44 kDa), isoform 2 (38 kDa), isoform 3 (39 kDa), isoform 4 (39 kDa), isoform 5 (34 kDa), and isoform 6 (34 kDa).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Dot Blot in non-acetylated and acetylated p53 peptides.

      Dot Blot Analysis: 1 µg/mL of this antibody detected acetyl-p53 in acetylated p53 peptides
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-acetyl-p53 (Lys120) Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-acetyl-p53 (Lys120) Antibody Certificates of Analysis

      TitleLot Number
      Anti-acetyl-p53 (Lys120) - Q2150356 Q2150356
      Anti-acetyl-p53 (Lys120) - 2520377 2520377
      Anti-acetyl-p53 (Lys120) - 3311810 3311810
      Anti-acetyl-p53 (Lys120) - 3589764 3589764
      Anti-acetyl-p53 (Lys120) -2779744 2779744
      Anti-acetyl-p53 (Lys120) -2792424 2792424
      Anti-acetyl-p53 (Lys120) -2831333 2831333
      Anti-acetyl-p53 (Lys120) -2845110 2845110
      Anti-acetyl-p53 (Lys120) -2855902 2855902
      Anti-acetyl-p53 (Lys120) Polyclonal Antibody 2918010

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies