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AB4332 Anti-acetyl-MVH (Lys405) Antibody

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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, H, R, PoDB, WB, ICC, PIA, IPRbAffinity PurifiedPolyclonal Antibody
      Catalogue NumberAB4332
      DescriptionAnti-acetyl-MVH (Lys405) Antibody
      Alternate Names
      • Probable ATP-dependent RNA helicase DDX4
      • DEAD box protein 4
      • Mvh
      • Vasa homolog
      Background InformationMVH (also known as DDX4 or Vasa) is a member of the DEAD family of proteins and is a probable ATP-dependent RNA helicase. MVH is mostly localized in testicular germ cells in the cytoplasm of spermatocytes. MVH is a high-copy extragenic suppressor gene. MVH is shown to be significantly decreased in spermatozoa of men that are infertile and may have an important role in male fertility and in stem cell development.
      Product Information
      FormatAffinity Purified
      • Non-acetylated and acetylated MVH (Lys405)
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      ApplicationAnti-acetyl-MVH (Lys405) Antibody is a Rabbit Polyclonal Antibody for detection of acetyl-MVH (Lys405) also known as Probable ATP-dependent RNA helicase DDX4, DEAD box protein & has been validated in DB, WB, ICC, PIA & IP.
      Key Applications
      • Dot Blot
      • Western Blotting
      • Immunocytochemistry
      • Peptide Inhibition Assay
      • Immunoprecipitation
      Application NotesWestern Blot/Immunoprecipitation Analysis: A representative lot detected Flag-tagged acetyl-MVH (Lys405) in the presence of Myc-HAT/p46.

      Immunocytochemistry Analysis: A representative lot from an independent laboratory detected acetyl-MVH (Lys405) in mouse germ cells (I. Nagamori, Department of Pharmacology, University of California, Irvine).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to mouse MVH acetylated at Lys405.
      EpitopeAcetylated Lys405
      SpecificityThis antibody specifically recognizes wild type MVH acetylated at Lys405, but not the Lys405 mutant.
      Species Reactivity
      • Mouse
      • Human
      • Rat
      • Pig
      Species Reactivity NoteDemonstrated to react with Mouse and Human.
      Predicted to react with Rat and Porcine based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Ddx4
      • Vasa
      • Acetylation
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: May play a role in germ cell development. May play a role in sperm motility.

      CATALYTIC ACTIVITY: ATP + H2O = ADP + phosphate.

      SUBUNIT STRUCTURE: Found in a mRNP complex, at least composed of TDRD1, TDRD6, TDRD7 and DDX4. N-terminus interacts with RANBP9. Interacts with PIWIL2 and MAEL.
      Molecular Weight76 kDa calculated
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Dot Blot/Peptide Inhibition Assay in non-acetylated and acetylated MVH (Lys405).

      Dot Blot/Peptide Inhibition Analysis: A 1:1,000 dilution of this antibody detected acetyl-MVH (Lys405) peptide.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information

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      Life Science Research > Antibodies and Assays > Primary Antibodies