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05-515 Anti-acetyl-Lysine Antibody, clone 4G12

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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, R, H, VrtIP, WBMPurifiedMonoclonal Antibody
      Catalogue Number05-515
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-acetyl-Lysine Antibody, clone 4G12
      Background InformationIn the nucleus, DNA is tightly packed into nucleosomes generating an environment which is highly repressive towards DNA processes such as transcription. Acetylation of lysine residues within proteins has emerged as an important mechanism used by cells to overcome this repression. The acetylation of non-histone proteins such as transcription factors, as well as histones appears to be involved in this process. Acetylation may result in structural transitions as well as specific signaling within discrete chromatin domains. The role of acetylation in intracellular signaling has been inferred from the binding of acetylated peptides by the conserved bromodomain. Furthermore, recent findings suggest that bromodomain/acetylated-lysine recognition can serve as a regulatory mechanism in protein-protein interactions in numerous cellular processes such as chromatin remodeling and transcriptional activation.
      Product Information
      • TSA-treated HeLa and NIH/3T3 cells, osteosarcoma tissue.
      PresentationPurified mouse monoclonal IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide, before the addition of glycerol to 30%.
      Quality LevelMQ100
      ApplicationDetect acetyl-Lysine with Anti-acetyl-Lysine Antibody, clone 4G12 (Mouse Monoclonal Antibody), that has been shown to work in IP & WB.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation:
      5 μg of a previous lot immunoprecipitated in vitro acetylated PCAF added to a 3T3 RIPA cell lysate. The immunoprecipitated PCAF was detected by subsequent western blot analysis using 1 μg/mL monoclonal anti-GST (Catalog # 05-311).
      Biological Information
      ImmunogenMixture of chemically acetylated antigens; recognizes numerous proteins acetylated on a lysine, most prominently on histones.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityAcetylated lysine-containing proteins including histones. Of all the possible H3 and H4 acetylation sites, this antibody exhibits highest affinity for histone H4 acetylated on lysine 8 and histone H3 acetylated on lysine 14.
      Species Reactivity
      • Mouse
      • Rat
      • Human
      • Vertebrates
      Species Reactivity NoteHuman. Expected to cross-react with rat and mouse.
      Antibody TypeMonoclonal Antibody
      • Acetylation
      Purification MethodProtein G Chromatography
      Molecular WeightVaries depending upon the protein being detected.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on untreated and sodium butyrated treated HeLa lysates.

      Western Blot Analysis:
      1:500 dilution of this lot detected ACETYL-LYSINE on 10 μg of sodium butyrated treated HeLa lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information


      Anti-acetyl-Lysine Antibody, clone 4G12 SDS


      Safety Data Sheet (SDS) 

      Anti-acetyl-Lysine Antibody, clone 4G12 Certificates of Analysis

      TitleLot Number
      Anti-acetyl-Lysine, clone 4G12 2475725
      Anti-acetyl-Lysine, clone 4G12 2834776
      Anti-acetyl-Lysine, clone 4G12 - 2138009 2138009
      Anti-acetyl-Lysine, clone 4G12 - 2391104 2391104
      Anti-acetyl-Lysine, clone 4G12 - 2452518 2452518
      Anti-acetyl-Lysine, clone 4G12 - 18728 18728
      Anti-acetyl-Lysine, clone 4G12 - 1958113 1958113
      Anti-acetyl-Lysine, clone 4G12 - 2016027 2016027
      Anti-acetyl-Lysine, clone 4G12 - 2056011 2056011
      Anti-acetyl-Lysine, clone 4G12 - 21532 21532


      Reference overviewApplicationPub Med ID
      HDAC1 activates FoxO and is both sufficient and required for skeletal muscle atrophy.
      Beharry, AW; Sandesara, PB; Roberts, BM; Ferreira, LF; Senf, SM; Judge, AR
      Journal of cell science  127  1441-53  2014

      Show Abstract
      Western Blotting24463822 24463822
      DNA damage signals through differentially modified E2F1 molecules to induce apoptosis.
      Carnevale, J; Palander, O; Seifried, LA; Dick, FA
      Molecular and cellular biology  32  900-12  2012

      Show Abstract
      Western Blotting22184068 22184068
      GmPHD5 acts as an important regulator for crosstalk between histone H3K4 di-methylation and H3K14 acetylation in response to salinity stress in soybean.
      Wu, T; Pi, EX; Tsai, SN; Lam, HM; Sun, SM; Kwan, YW; Ngai, SM
      BMC plant biology  11  178  2011

      Show Abstract
      22168212 22168212
      p300 Acetyltransferase activity differentially regulates the localization and activity of the FOXO homologues in skeletal muscle.
      Senf, SM; Sandesara, PB; Reed, SA; Judge, AR
      American journal of physiology. Cell physiology  300  C1490-501  2011

      Show Abstract
      21389279 21389279
      SIRT1 acts as a modulator of neointima formation following vascular injury in mice.
      Li, L; Zhang, HN; Chen, HZ; Gao, P; Zhu, LH; Li, HL; Lv, X; Zhang, QJ; Zhang, R; Wang, Z; She, ZG; Zhang, R; Wei, YS; Du, GH; Liu, DP; Liang, CC
      Circulation research  108  1180-9  2011

      Show Abstract
      Western Blotting21474819 21474819
      Aurora B interacts with NIR-p53, leading to p53 phosphorylation in its DNA-binding domain and subsequent functional suppression.
      Wu, L; Ma, CA; Zhao, Y; Jain, A
      The Journal of biological chemistry  286  2236-44  2011

      Show Abstract
      20959462 20959462
      Chromatin loading of E2F-MLL complex by cancer-associated coregulator ANCCA via reading a specific histone mark.
      Revenko, AS; Kalashnikova, EV; Gemo, AT; Zou, JX; Chen, HW
      Molecular and cellular biology  30  5260-72  2010

      Show Abstract Full Text Article
      20855524 20855524
      CCAAT/enhancer binding protein beta2 is involved in growth hormone-regulated insulin-like growth factor-II gene expression in the liver of rainbow trout (Oncorhynchus mykiss).
      Lo, JH; Chen, TT
      Endocrinology  151  2128-39  2010

      Show Abstract
      20228168 20228168
      AAA+ proteins RUVBL1 and RUVBL2 coordinate PIKK activity and function in nonsense-mediated mRNA decay.
      Izumi N, Yamashita A, Iwamatsu A, Kurata R, Nakamura H, Saari B, Hirano H, Anderson P, Ohno S
      Sci Signal  3  ra27.  2010

      Show Abstract
      20371770 20371770
      Contrasting proteome biology and functional heterogeneity of the 20 S proteasome complexes in mammalian tissues.
      Gomes, AV; Young, GW; Wang, Y; Zong, C; Eghbali, M; Drews, O; Lu, H; Stefani, E; Ping, P
      Molecular & cellular proteomics : MCP  8  302-15  2009

      Show Abstract Full Text Article
      18931337 18931337

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      Life Science Research > Antibodies and Assays > Primary Antibodies