06-1372 Anti-acetyl (Lys26) phospho (Ser27) Histone H1.4 Antibody

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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, DB Rb Affinity Purified Polyclonal Antibody
      Catalogue Number06-1372
      DescriptionAnti-acetyl (Lys26) phospho (Ser27) Histone H1.4 Antibody
      Alternate Names
      • Histone H1.4
      • Histone H1b
      Background InformationH1.4 is one of four variants of histone H1 (H1.2-H1.5) that is found in somatic cells. The H1 histones may function as linker proteins binding DNA via their GH domain, and enabling the wrapping of DNA around the core nucleosomal structure formed from the association of H2A, H2B, H3, and H4. In response to various stimuli, histones undergo a number of post-translational modifications (PTM) including acetylation, phoshorylation, and methylation. Previous studies have suggested that H1.4 may be phosphorylated during mitosis and interphase resulting in increased transcription of target genes by RNA polymerase I and II, and therefore this PTM on H1.4 may have implications for cell growth. H1.4 may also undergo acetylation of its N-terminal lysine residues. Often, it is the pattern of different modifications on several histone residues that alter chromatin and affect gene expression, rather than modification of single residues.
      Product Information
      FormatAffinity Purified
      • Colcemid treated HeLa acid extract
      PresentationPurified rabbit polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      ApplicationAnti-acetyl (Lys26) phospho (Ser27) Histone H1.4 Antibody is a rabbit polyclonal antibody for detection of acetyl (Lys26) phospho (Ser27) Histone H1.4 also known as Histone H1.4, Histone H1b & has been validated in WB, DB.
      Key Applications
      • Western Blotting
      • Dot Blot
      Application NotesDot Blot Analysis: A 1:1,320 dilution from representative lot detected acetyl (Lys26) phospho (Ser27) Histone H1.4 peptides.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the N-terminus of human Histone H1.4 acetylated at Lys26 and phosphorylated at Ser27.
      SpecificityThis antibody recognizes the N-terminus of Histone H1.4 acetylated at Lys26 and phosphorylated at Ser27.
      Species Reactivity
      • Human
      Species Reactivity NoteDemonstrated to react with Human. Predicted to react with Canine, Orangutan, Giant Panda, and Rhesus Macaque based on 100% sequence homology. Other homologies: Rat and Mouse (82% sequence homology).
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HIST1H1E
      • H1F4
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~35 kDa observed. Uncharacterized bands may be observed at ~70 kDa and ~17 kDa in some acid extracts.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in colcemid treated HeLa acid extract.

      Western Blot Analysis: A 1:3,000 dilution of this antibody detected acetyl (Lys26) phospho (Ser27) Histone H1.4 in 10 µg of colcemid treated HeLa acid extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information


      Anti-acetyl (Lys26) phospho (Ser27) Histone H1.4 Antibody Certificates of Analysis

      TitleLot Number
      Anti-acetyl (Lys26) phospho (Ser27) -27809292780929
      Anti-acetyl (Lys26) phospho (Ser27) Histone H1.4 - 24513262451326
      Anti-acetyl (Lys26) phospho (Ser27) Histone H1.4 - 24671452467145
      Anti-acetyl (Lys26) phospho (Ser27) Histone H1.4 - Q2032349Q2032349


      Reference overviewPub Med ID
      Dynamics and dispensability of variant-specific histone H1 Lys-26/Ser-27 and Thr-165 post-translational modifications.
      Terme, Jean-Michel, et al.
      FEBS Lett., (2014)  2014

      Show Abstract
      24873882 24873882

      Technical Info

      White Paper - The Message in the Marks: Deciphering Cancer Epigenetics (EMD)

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      Life Science Research > Antibodies and Assays > Primary Antibodies