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06-599 Anti-acetyl-Histone H3 Antibody

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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, ICC, ChIP-seq, ChIPRbPurifiedPolyclonal Antibody
      Catalogue Number06-599
      DescriptionAnti-acetyl-Histone H3 Antibody
      Alternate Names
      • H3 histone family, member T
      • histone 3, H3
      • histone cluster 3, H3
      Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Acetylation of histone H3 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs). Acetylation of lysine14 is commonly seen in genes that are being actively transcribed into RNA.
      Product Information
      • HeLa acid extract, sodium butyrate treated HeLa cells, and recombinant Histone H3
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      ApplicationDetect acetyl-Histone H3 with Anti-acetyl-Histone H3 Antibody (Rabbit Polyclonal Antibody), that has been shown to work in WB, ICC, ChIP, ChIP-seq.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • ChIP-seq
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesImmunocytochemistry Analysis: 1:500 dilution of a representative lot of this antibody detected acetylated Histone H3 in NIH/3T3 and A431 cells.

      Chromatin Immunoprecipitation: This antibody has been shown to work for chIP using HeLa extracted chromatin and 5 mg of this antibody.

      ChIP-Seq: This antibody has been shown to work for ChIP-Seq in Kanai, A., et. al. (2011). DNA Research 18:379-392.
      Biological Information
      ImmunogenLinear peptide corresponding to human Histone H3 acetylated at the N-terminus.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityRecognizes Histone H3 acetylated at the N-terminus.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteDemonstrated to react with human, mouse, and rat. Broad species cross-reactivity expected based on sequence similarity.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq].
      Gene Symbol
      • HIST3H3
      • H3FT
      • H3t
      • H3T
      • H3/g
      • H3.4
      • H3/t
      • Acetylation
      UniProt Number
      UniProt SummaryFUNCTION: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

      SIZE: 136 amino acids; 15508 Da

      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.


      TISSUE SPECIFICITY: Expressed in testicular cells.

      DEVELOPMENTAL STAGE: Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.

      PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity).

      SIMILARITY: Belongs to the histone H3 family.
      Molecular Weight~ 17 kDa observed
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa acid extract, sodium butyrate treated HeLa cells, and recombinant Histone H3.

      Western Blot Analysis: 0.05 µg/mL of this antibody detected Histone H3 on 10 µg of HeLa acid extract, sodium butyrate treated HeLa cells, and recombinant Histone H3.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Global Trade Item Number
      Catalog Number GTIN
      06-599 04053252406812


      Anti-acetyl-Histone H3 Antibody SDS


      Safety Data Sheet (SDS) 

      Anti-acetyl-Histone H3 Antibody Certificates of Analysis

      TitleLot Number
      Anti-acetyl-Histone H3 - 2153150 2153150
      Anti-acetyl-Histone H3 - 2370129 2370129
      Anti-acetyl-Histone H3 - 2430399 2430399
      Anti-acetyl-Histone H3 - 2455632 2455632
      Anti-acetyl-Histone H3 - 15792 15792
      Anti-acetyl-Histone H3 - 17300 17300
      Anti-acetyl-Histone H3 - 19165 19165
      Anti-acetyl-Histone H3 - 1969119 1969119
      Anti-acetyl-Histone H3 - 2068182 2068182
      Anti-acetyl-Histone H3 - 21514 21514


      Reference overviewApplicationSpeciesPub Med ID
      Compensatory induction of MYC expression by sustained CDK9 inhibition via a BRD4-dependent mechanism.
      Lu, H; Xue, Y; Xue, Y; Yu, GK; Arias, C; Lin, J; Fong, S; Faure, M; Weisburd, B; Ji, X; Mercier, A; Sutton, J; Luo, K; Gao, Z; Zhou, Q
      eLife  4  e06535  2015

      Show Abstract
      26083714 26083714
      SCARECROW-LIKE15 interacts with HISTONE DEACETYLASE19 and is essential for repressing the seed maturation programme.
      Gao, MJ; Li, X; Huang, J; Gropp, GM; Gjetvaj, B; Lindsay, DL; Wei, S; Coutu, C; Chen, Z; Wan, XC; Hannoufa, A; Lydiate, DJ; Gruber, MY; Chen, ZJ; Hegedus, DD
      Nature communications  6  7243  2015

      Show Abstract
      26129778 26129778
      Lysine-specific demethylase (LSD1/KDM1A) and MYCN cooperatively repress tumor suppressor genes in neuroblastoma.
      Amente, S; Milazzo, G; Sorrentino, MC; Ambrosio, S; Di Palo, G; Lania, L; Perini, G; Majello, B
      Oncotarget  6  14572-83  2015

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      Salicylic acid biosynthesis is enhanced and contributes to increased biotrophic pathogen resistance in Arabidopsis hybrids.
      Yang, L; Li, B; Zheng, XY; Li, J; Yang, M; Dong, X; He, G; An, C; Deng, XW
      Nature communications  6  7309  2015

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      26065719 26065719
      DOT1L cooperates with the c-Myc-p300 complex to epigenetically derepress CDH1 transcription factors in breast cancer progression.
      Cho, MH; Park, JH; Choi, HJ; Park, MK; Won, HY; Park, YJ; Lee, CH; Oh, SH; Song, YS; Kim, HS; Oh, YH; Lee, JY; Kong, G
      Nature communications  6  7821  2015

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      HDAC8, A Potential Therapeutic Target for the Treatment of Malignant Peripheral Nerve Sheath Tumors (MPNST).
      Lopez, G; Bill, KL; Bid, HK; Braggio, D; Constantino, D; Prudner, B; Zewdu, A; Batte, K; Lev, D; Pollock, RE
      PloS one  10  e0133302  2015

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      Checkpoint Activation of an Unconventional DNA Replication Program in Tetrahymena.
      Sandoval, PY; Lee, PH; Meng, X; Kapler, GM
      PLoS genetics  11  e1005405  2015

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      26218270 26218270
      PU.1 Suppresses Th2 Cytokine Expression via Silencing of GATA3 Transcription in Dendritic Cells.
      Yashiro, T; Kubo, M; Ogawa, H; Okumura, K; Nishiyama, C
      PloS one  10  e0137699  2015

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      26361334 26361334
      The Depsipeptide Romidepsin Reverses HIV-1 Latency In Vivo.
      Søgaard, OS; Graversen, ME; Leth, S; Olesen, R; Brinkmann, CR; Nissen, SK; Kjaer, AS; Schleimann, MH; Denton, PW; Hey-Cunningham, WJ; Koelsch, KK; Pantaleo, G; Krogsgaard, K; Sommerfelt, M; Fromentin, R; Chomont, N; Rasmussen, TA; Østergaard, L; Tolstrup, M
      PLoS pathogens  11  e1005142  2015

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      Nanocurcumin Prevents Hypoxia Induced Stress in Primary Human Ventricular Cardiomyocytes by Maintaining Mitochondrial Homeostasis.
      Nehra, S; Bhardwaj, V; Ganju, L; Saraswat, D
      PloS one  10  e0139121  2015

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      Technical Info

      White Paper - The Message in the Marks: Deciphering Cancer Epigenetics