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06-599 Anti-acetyl-Histone H3 Antibody

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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R WB, ICC, ChIP-seq, ChIP Rb Purified Polyclonal Antibody
      Catalogue Number06-599
      DescriptionAnti-acetyl-Histone H3 Antibody
      Alternate Names
      • H3 histone family, member T
      • histone 3, H3
      • histone cluster 3, H3
      Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Acetylation of histone H3 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs). Acetylation of lysine14 is commonly seen in genes that are being actively transcribed into RNA.
      Product Information
      • HeLa acid extract, sodium butyrate treated HeLa cells, and recombinant Histone H3
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      ApplicationDetect acetyl-Histone H3 with Anti-acetyl-Histone H3 Antibody (Rabbit Polyclonal Antibody), that has been shown to work in WB, ICC, ChIP, ChIP-seq.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • ChIP-seq
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesImmunocytochemistry Analysis: 1:500 dilution of a representative lot of this antibody detected acetylated Histone H3 in NIH/3T3 and A431 cells.

      Chromatin Immunoprecipitation: This antibody has been shown to work for chIP using HeLa extracted chromatin and 5 mg of this antibody.

      ChIP-Seq: This antibody has been shown to work for ChIP-Seq in Kanai, A., et. al. (2011). DNA Research 18:379-392.
      Biological Information
      ImmunogenLinear peptide corresponding to human Histone H3 acetylated at the N-terminus.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityRecognizes Histone H3 acetylated at the N-terminus.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteDemonstrated to react with human, mouse, and rat. Broad species cross-reactivity expected based on sequence similarity.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq].
      Gene Symbol
      • HIST3H3
      • H3FT
      • H3t
      • H3T
      • H3/g
      • H3.4
      • H3/t
      • Acetylation
      UniProt Number
      UniProt SummaryFUNCTION: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

      SIZE: 136 amino acids; 15508 Da

      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.


      TISSUE SPECIFICITY: Expressed in testicular cells.

      DEVELOPMENTAL STAGE: Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.

      PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity).

      SIMILARITY: Belongs to the histone H3 family.
      Molecular Weight~ 17 kDa observed
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa acid extract, sodium butyrate treated HeLa cells, and recombinant Histone H3.

      Western Blot Analysis: 0.05 µg/mL of this antibody detected Histone H3 on 10 µg of HeLa acid extract, sodium butyrate treated HeLa cells, and recombinant Histone H3.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information


      Anti-acetyl-Histone H3 Antibody Certificates of Analysis

      TitleLot Number
      Anti-acetyl-Histone H3 - 21531502153150
      Anti-acetyl-Histone H3 - 23701292370129
      Anti-acetyl-Histone H3 - 24303992430399
      Anti-acetyl-Histone H3 - 24556322455632
      Anti-acetyl-Histone H3 - 1579215792
      Anti-acetyl-Histone H3 - 1730017300
      Anti-acetyl-Histone H3 - 1916519165
      Anti-acetyl-Histone H3 - 19691191969119
      Anti-acetyl-Histone H3 - 20681822068182
      Anti-acetyl-Histone H3 - 2151421514


      Reference overviewApplicationSpeciesPub Med ID
      PU.1 Suppresses Th2 Cytokine Expression via Silencing of GATA3 Transcription in Dendritic Cells.
      Yashiro, T; Kubo, M; Ogawa, H; Okumura, K; Nishiyama, C
      PloS one  10  e0137699  2015

      Show Abstract
      26361334 26361334
      The Depsipeptide Romidepsin Reverses HIV-1 Latency In Vivo.
      Søgaard, OS; Graversen, ME; Leth, S; Olesen, R; Brinkmann, CR; Nissen, SK; Kjaer, AS; Schleimann, MH; Denton, PW; Hey-Cunningham, WJ; Koelsch, KK; Pantaleo, G; Krogsgaard, K; Sommerfelt, M; Fromentin, R; Chomont, N; Rasmussen, TA; Østergaard, L; Tolstrup, M
      PLoS pathogens  11  e1005142  2015

      Show Abstract
      26379282 26379282
      Myeloid-derived suppressor cells impair alveolar macrophages through PD-1 receptor ligation during Pneumocystis pneumonia.
      Lei, GS; Zhang, C; Lee, CH
      Infection and immunity  83  572-82  2015

      Show Abstract
      25404033 25404033
      Nanocurcumin Prevents Hypoxia Induced Stress in Primary Human Ventricular Cardiomyocytes by Maintaining Mitochondrial Homeostasis.
      Nehra, S; Bhardwaj, V; Ganju, L; Saraswat, D
      PloS one  10  e0139121  2015

      Show Abstract
      26406246 26406246
      A gene expression signature identifying transient DNMT1 depletion as a causal factor of cancer-germline gene activation in melanoma.
      Cannuyer, J; Van Tongelen, A; Loriot, A; De Smet, C
      Clin Epigenetics  7  114  2015

      Show Abstract
      26504497 26504497
      Progenitor stage-specific activity of a cis-acting double GATA motif for Gata1 gene expression.
      Moriguchi, T; Suzuki, M; Yu, L; Takai, J; Ohneda, K; Yamamoto, M
      Molecular and cellular biology  35  805-15  2015

      Show Abstract
      25535330 25535330
      Circovirus transport proceeds via direct interaction of the cytoplasmic dynein IC1 subunit with the viral capsid protein.
      Cao, J; Lin, C; Wang, H; Wang, L; Zhou, N; Jin, Y; Liao, M; Zhou, J
      Journal of virology  89  2777-91  2015

      Show Abstract
      25540360 25540360
      Epigenetic heterogeneity in HIV-1 latency establishment.
      Matsuda, Y; Kobayashi-Ishihara, M; Fujikawa, D; Ishida, T; Watanabe, T; Yamagishi, M
      Scientific reports  5  7701  2015

      Show Abstract
      25572573 25572573
      hnRNP K coordinates transcriptional silencing by SETDB1 in embryonic stem cells.
      Thompson, PJ; Dulberg, V; Moon, KM; Foster, LJ; Chen, C; Karimi, MM; Lorincz, MC
      PLoS genetics  11  e1004933  2015

      Show Abstract
      Western Blotting25611934 25611934
      An IL-27/NFIL3 signalling axis drives Tim-3 and IL-10 expression and T-cell dysfunction.
      Zhu, C; Sakuishi, K; Xiao, S; Sun, Z; Zaghouani, S; Gu, G; Wang, C; Tan, DJ; Wu, C; Rangachari, M; Pertel, T; Jin, HT; Ahmed, R; Anderson, AC; Kuchroo, VK
      Nature communications  6  6072  2015

      Show Abstract
      25614966 25614966

      Technical Info

      White Paper - The Message in the Marks: Deciphering Cancer Epigenetics (EMD)