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09-881 Anti-acetyl Cortactin Antibody

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09-881
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number09-881
      DescriptionAnti-acetyl Cortactin Antibody
      Alternate Names
      • Amplaxin
      • Oncogene EMS1
      • Src substrate cortactin
      Background InformationSrc substrate cortactin (UniProt: Q14247; also known as Amplaxin, Oncogene EMS1) is encoded by the CTTN (also known as EMS) gene (Gene ID: 2017) in human. Cortactin is a peripheral membrane protein that serves as a substrate for Src tyrosine kinase and plays a role in regulating cell motility. It is shown to interact with F-actin to promote its polymerization and branching and contributes to the organization of the actin cytoskeleton and cell shape. It also plays a role in the formation of lamellipodia and in cell migration. It contains an N-terminal acidic domain, six and a half tandem repeats of a unique 37-amino acid sequence, and a C-terminal Src homology (SH3) domain. The repeat regions are shown to be essential for F-actin binding. Cortactin is activated via phosphorylation by upstream kinases in response to extracellular stimuli. In mice, Src kinase primarily phosphorylates cortactin at tyrosines 421, 466, and 482, which leads its interaction with F-actin and Arp2/3. It can also be phosphorylated by ERK on serine 405 and 418 that results in recruitment of additional actin nucleation-promoting factors (NPFs), most importantly N-WASP, to the Arp2/3/ complex. Deacetylation of cortactin by HDAC6 is reported to block its translocation to the cell periphery and block its association with F-actin and impairs cell motility. Overexpression of cortactin has been reported in many human tumors and its expression correlates well with the metastatic potential of breast cancer cells and hepatocellular carcinomas. (Ref.: Zhang, X., et al. (2007). Mol. Cell. 27(2); 197-213; Chuma, M., et al. (2004). J. Hepatol. 41(4); 629-636; Li, Y., et al. (2001). Cancer Res. 61(18); 6906-6911; Meiler, E. et al. (2012). PLoS One. 7(3); e33662).
      References
      Product Information
      FormatAffinity Purified
      Control
      • HeLa-Anti-Cortactin immunocomplex treated with TSA/Nicotinamide
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-acetyl Cortactin, Cat. No. 09-881, is a rabbit polyclonal antibody that detects Acetyl-Cortactin and is tested for use in Western Blotting.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated linear peptide surrounding acetylated lysine site of human cortactin.
      EpitopeUnknown
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody specifically detects acetylated form of Cortactin.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman and mouse. Predicted to react with rat and drosophila based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Two splice variants that encode different isoforms have been identified for this gene. [provided by RefSeq]
      Gene Symbol
      • CTTN
      • EMS1
      Modifications
      • Acetylation
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: May contribute to the organization of cell structure. The SH3 motif may function as a binding region to cytoskeleton. Tyrosine phosphorylation in transformed cells may contribute to cellular growth regulation and transformation.

      SUBUNIT STRUCTURE: Interacts with SHANK2 and SHANK3 via its SH2 domain. Also interacts with FGD1 By similarity. Interacts with PLXDC2.

      SUBCELLULAR LOCATION: Cytoplasm › cytoskeleton By similarity. Cell projection › lamellipodium By similarity. Cell projection › ruffle By similarity. Note: Associated with membrane ruffles and lamellipodia By similarity.

      SEQUENCE SIMILARITIES: Contains 7 cortactin repeats.

      Contains 1 SH3 domain.
      Molecular Weight~80 kDa observed; 61.59 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in lysate from HeLa cells treated with Trichostatin A and Nicotinamide.

      Western Blotting Analysis: A 1:400 dilution of this antibody detected immunoprecipitated Acetyl-Cortactin in lysate from HeLa cells treated with 13.2 mM Trichostatin A and 20 mM Nicotinamide (12 h), but not in lysate from untreated cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-acetyl Cortactin Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-acetyl Cortactin Antibody Certificates of Analysis

      TitleLot Number
      Anti-acetyl Cortactin 2453216
      Anti-acetyl Cortactin - 2012966 2012966
      Anti-acetyl Cortactin - 2028604 2028604
      Anti-acetyl Cortactin - 2120096 2120096
      Anti-acetyl Cortactin - 2280478 2280478
      Anti-acetyl Cortactin - 2489040 2489040
      Anti-acetyl Cortactin - 3467106 3467106
      Anti-acetyl Cortactin - 3610572 3610572
      Anti-acetyl Cortactin - 3698814 3698814
      Anti-acetyl Cortactin - 3700478 3700478

      References

      Reference overviewPub Med ID
      Cortactin tyrosine phosphorylation promotes its deacetylation and inhibits cell spreading.
      Meiler, Eugenia, et al.
      PLoS ONE, 7: e33662 (2012)  2012

      Show Abstract
      22479425 22479425

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies