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MABE1124 Anti-XPA Antibody, clone 1XPA Antibody-1E11

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MABE1124
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, ICC M Ascites Monoclonal Antibody
      Description
      Catalogue NumberMABE1124
      DescriptionAnti-XPA Antibody, clone 1XPA Antibody-1E11
      Alternate Names
      • DNA repair protein complementing XP-A cells
      • XPA
      • Xeroderma pigmentosum group A-complementing protein
      Background InformationDNA repair protein complementing XP-A cells (UniProt P23025; also known as Excision repair-controlling, Mutant xeroderma pigmentosum complementation group A, Xeroderma pigmentosum group A-complementing protein) is encoded by the XPA (also known as XP1, XPAC) gene (Gene ID 7507) in human. DNA lesions caused by UV irradiation, drugs, or other environmental factors are eliminated by two nucleotide excision repair (NER) pathways, Global genome repair (GGR) and transcription-coupled repair (TCR). In GGR, the removal of lesions requires their recognition by the repair factor XPC/HR23b and the subsequent opening of the DNA duplex by transcription factor II human (TFIIH). The resulting single-stranded structure is stabilized by XPA and replication protein A (RPA). XPG is recruited through its interaction with TFIIH on the 3′ side of the lesion and its positioning on the cut site requires RPA. The interaction between XPA and XPB (ERCC1) stimulates the recruitment of ERCC1-XPF on the 5′ side of the DNA lesion. The damaged oligonucleotide can then be removed through the double incision by XPG and ERCC1-XPF endonucleases. In TCR, these factors (except XPC/HR23B) are recruited by the stalled RNA pol II in front of the damage with the help of the CSB and CSA proteins.
      References
      Product Information
      FormatAscites
      PresentationMouse monoclonal IgG1κ ascites with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-XPA Antibody, clone 1XPA Antibody-1E11 is an antibody against XPA for use in Western Blotting, Immunocytochemistry.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesWestern Blotting Analysis: A representative lot detected the ATP-dependent recruitment of XPA in HeLa nuclear extract onto the stalled RNA pol IIO on mono-cisplatin-damaged DNA (Riedl, T., et al. (2003). EMBO J. 22(19):5293-5303; Laine, J.P., and, Egly, J.M. (2006). EMBO J. 25(2):3873-97).
      Immunocytochemistry Analysis: A representative lot detected XPA recruitment to the DNA damage sites in the nuclei of UV-irradated HeLa cells (Alekseev, S., et al. (2014). Chem Biol. 21(3):398-407).
      Biological Information
      ImmunogenLinear peptide corresponding to human XPA sequence near C-terminus.
      EpitopeNear C-terminus
      Clone1XPA-1E11
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • XPA
      • XPAC
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~37 kDa observed. Target band appears larger than the calculated molecular weight of 31.4 kDa, most likely due to posttranslational modifications. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa nuclear extract.

      Western Blotting Analysis: A 1:2,000 dilution of this antibody detected XPA in 10 µg of HeLa nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-XPA Antibody, clone 1XPA Antibody-1E11 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-XPA Antibody, clone 1XPA Antibody-1E11 Certificates of Analysis

      TitleLot Number
      Anti-XPA, clone 1XPA-1E11 -Q2589914Q2589914

      References

      Reference overviewPub Med ID
      A small molecule screen identifies an inhibitor of DNA repair inducing the degradation of TFIIH and the chemosensitization of tumor cells to platinum.
      Alekseev, S; Ayadi, M; Brino, L; Egly, JM; Larsen, AK; Coin, F
      Chemistry & biology  21  398-407  2014

      Show Abstract
      24508195 24508195
      Initiation of DNA repair mediated by a stalled RNA polymerase IIO.
      Lainé, JP; Egly, JM
      The EMBO journal  25  387-97  2006

      Show Abstract
      16407975 16407975
      The comings and goings of nucleotide excision repair factors on damaged DNA.
      Riedl, T; Hanaoka, F; Egly, JM
      The EMBO journal  22  5293-303  2003

      Show Abstract
      14517266 14517266