PC234L Anti-Vasopressin Rabbit pAb

Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
R Rb Polyclonal Antibody

Pricing & Availability

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PC234L-100UL
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      Plastic ampoule 100 ul
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      Description
      OverviewRecognizes vasopressin in rat hypothalamus.
      Catalogue NumberPC234L
      Brand Family Calbiochem®
      Application Data
      Detection of rat vasopressin by immunohistochemistry. Sample: Rat paraventricular nucleus and supraoptic nucleus. Primary antibody: Anti-Vasopressin Rabbit pAb (Cat. No. PC234L) (1:400). Detection: fluorescence.
      References
      ReferencesSudhof, T.C. 1995. Nature 375, 645.
      Hokfelt, T., et al. 1987. Experientia Suppl. 56, 154.
      Product Information
      FormLyophilized
      FormulationUndiluted serum.
      Positive controlRat hypothalamus
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Frozen Sections
      Immunofluorescence
      Application NotesFrozen Sections (1:400-1:800, Cy3; 1:2000-1:4000, Bn-SA/HRP)
      Immunofluorescence (1:200-1:400)
      Application CommentsAntibody specificity was examined in the rat paraventricular nucleus and supraoptic nucleus. Staining is completely eliminated by pretreatment of antibody with arginine vasopressin (10 µg/ml). Preabsorption of antibody with oxytocin (100 µg/ml) resulted in no loss of immunostaining. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenrat arginine vasopressin
      ImmunogenRat
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Rat
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsReconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Be careful to reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months. Avoid freeze/thaw cycles of solutions.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Vasopressin Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Vasopressin Rabbit pAb Certificates of Analysis

      TitleLot Number
      PC234L

      References

      Reference overview
      Sudhof, T.C. 1995. Nature 375, 645.
      Hokfelt, T., et al. 1987. Experientia Suppl. 56, 154.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision20-April-2009 JSW
      ApplicationFrozen Sections (1:400-1:800, Cy3; 1:2000-1:4000, Bn-SA/HRP)
      Immunofluorescence (1:200-1:400)
      Application Data
      Detection of rat vasopressin by immunohistochemistry. Sample: Rat paraventricular nucleus and supraoptic nucleus. Primary antibody: Anti-Vasopressin Rabbit pAb (Cat. No. PC234L) (1:400). Detection: fluorescence.
      DescriptionRabbit polyclonal antibody supplied as lyophilized undiluted serum. Recognizes vasopressin.
      BackgroundNeurotransmitters can be defined as chemical substances that are released by the presynaptic neuron before being bound by receptors on the postsynaptic cell surface. It has been well established that the nervous system utilizes neurotransmitters for cell signaling. Neurotransmitters can be divided into two groups; low molecular weight transmitter molecules such as biogenic amines, purines, or amino acids, and bioactive peptides, of which more than 50 members have been identified in the mammalian central nervous system. Many low molecular weight neurotransmitters are produced within the axon terminal from common cellular metabolites and packaged into synaptic vesicles. In contrast, neuropeptides are synthesized in the cell body and packaged into large dense core vesicles for delivery to the presynaptic membrane. The transport and delivery of vesicles containing neurotransmitter is a highly regulated and complex process. The neuropeptide vasopressin plays important roles in vasoconstriction, antinociception, behavior, electrolyte balance, and coagulation functions. Vasopressin helps regulate the hypothalamic-pituitary-adrenal axis along with the corticotropin-releasing hormone. The immuno-localization, neuronal distribution, and vesicular compartmentalization of neurotransmitter molecules and their precursors are important for understanding the modulation, regulation, and function of neurotransmitters in the nervous system.
      HostRabbit
      Immunogen speciesRat
      Immunogenrat arginine vasopressin
      IsotypeIgG
      Speciesrat
      Positive controlRat hypothalamus
      FormLyophilized
      FormulationUndiluted serum.
      Preservative≤0.1% sodium azide
      CommentsAntibody specificity was examined in the rat paraventricular nucleus and supraoptic nucleus. Staining is completely eliminated by pretreatment of antibody with arginine vasopressin (10 µg/ml). Preabsorption of antibody with oxytocin (100 µg/ml) resulted in no loss of immunostaining. Antibody should be titrated for optimal results in individual systems.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsReconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Be careful to reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months. Avoid freeze/thaw cycles of solutions.
      Toxicity Standard Handling
      ReferencesSudhof, T.C. 1995. Nature 375, 645.
      Hokfelt, T., et al. 1987. Experientia Suppl. 56, 154.