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AB2251-I Anti-VGluT2 Antibody

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AB2251-I
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, MWB, IHCGpSerumPolyclonal Antibody
      Description
      Catalogue NumberAB2251-I
      DescriptionAnti-VGluT2 Antibody
      Alternate Names
      • Vesicular glutamate transporter 2
      • Differentiation-associated BNPI
      • Differentiation-associated Na(+)-dependent inorganic phosphate cotransporte
      • Solute carrier family 17 member 6
      • VGluT2
      Background InformationVesicular glutamate transporter 2 (UniProt Q9JI12; also known as Differentiation-associated BNPI, Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter, Solute carrier family 17 member 6, VGluT2) is encoded by the Slc17a6 (also known as Dnpi, Vglut2) gene (Gene ID: 84487) in rat. VGluT2 is a multi-pass membrane protein that mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neurons. It contains seven cytoplasmic domains, twelve transmembrane domains, and six vesicular domains. Its N- and C-terminal tails (aa 1-71 and 499-582, respectively) are exposed at the cytoplasmic side. VGluT1- and VGluT2-expressing neurons represent the bulk of canonical glutamatergic neurons in the brain, with VGluT1 representing the major subtype and accounting for approximately 80% of total vesicular transport of glutamate in the brain. Expression of VGluT2 is reported to increase sharply during the first ten days of postnatal life, and also increases with increasing numbers of rhodopsin cells in the retina. It has been shown that both VGluT1 and VGluT2 are expressed prominently in the adult brain, however, during the first two weeks of postnatal development, VGluT2 expression predominates. VGluT1 expression becomes essential for survival at the time of the developmental switch from VGLUT2 to VGLUT1.
      References
      Product Information
      FormatSerum
      PresentationGuinea Pig polyclonal antibody in buffer containing serum with 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-VGluT2, Cat. No. AB2251-I is a Guinea pig polyclonal antibody that detects VGluT2 and is tested for use in Immunohistochemistry (Paraffin), and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunohistochemistry (Paraffin) Analysis: A 1:2,000 dilution from a representative lot detected VGluT2 in mouse brain and rat brain tissue sections.

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 18 amino acids from the C-terminal, cytoplasmic domain of rat Vesicular Glutamate Transporter 2 (VGluT2).
      EpitopeC-terminus.
      ConcentrationPlease refer to lot specific datasheet.
      HostGuinea Pig
      SpecificityThis Guinea Pig polyclonal antibody detects Vesicular glutamate transporter 2. It targets an epitope within 18 amino acids from the C-terminal region.
      Species Reactivity
      • Rat
      • Mouse
      Species Reactivity NoteRat, Mouse.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Slc17a6
      • Dnpi
      • Vglut2
      Purification MethodUnpurified.
      UniProt Number
      Molecular Weight~56 kDa observed; 64.58 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Rat Brain Membrane tissue lysate.

      Western Blotting Analysis: A 1:10,000 dilution of this antibody detected VGluT2 in Rat Brain Membrane tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      AB2251-I 04055977355345

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies