07-1420 Anti-VEGF Antibody

50 µg  
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      Replacement Information
      Replacement Information07-1420 is a recommended replacement for AB2142P.

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M WB, IH(P), ELISA Rb Affinity Purified Polyclonal Antibody
      Catalogue Number07-1420
      DescriptionAnti-VEGF Antibody
      Alternate Names
      • Vascular permeability factor
      Background InformationVEGF (Vascular endothelial growth factor, VEGFA, VEGF-A), a dimeric ligand, is among the most potent angiogenic mitogens. VEGF is secreted by tumor cells and other cells exposed to hypoxia. VEGF is a highly specific mitogen for vascular endothelial cells. Seven VEGF isoforms (a, b, c, d, e, f, g) are generated as a result of alternative splicing from a single VEGF gene. The most commonly studied isoforms are VEGF121, VEGF165, and VEGF189 (f, d and b, respectively) All seven isoforms differ in their molecular mass and in biological properties such as their ability to bind to cell-surface heparan-sulfate proteoglycans. The expression of VEGF is potentiated and is secreted by tumor cells in response to hypoxia, by activated oncogenes, and by a variety of cytokines. VEGF induces angiogenesis as well as permeabilization of blood vessels, and plays a central role in the regulation of vasculogenesis. VEGF expression correlates to the degree of tumor vascularization and with increased metastatic risk. Additionally, VEGF induces endothelial cell proliferation, promotes cell migration, and inhibits apoptosis. Consequently, inhibition of VEGF signaling abrogates the development of a wide variety of tumors.
      Product Information
      FormatAffinity Purified
      • Huvec cell lysate
      PresentationAffinity purified rabbit polyclonal IgG employing immobilized mouse VEGF matrix. Antibody solution (1 mg/ml) was lyophilized from PBS, pH 7.2. Reconstitute with sterile distilled water.
      ApplicationAnti-VEGF Antibody detects level of VEGF & has been published & validated for use in WB, IH(P), ELISA.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      • ELISA
      Application NotesImmunohistochemisty (paraffin): VEGF staining in mouse placenta, tissue was pretreated with citrate buffer, pH 6.0. A previous lot of antibody was diluted to 1:100, using IHC-Select® detection with HRP-DAB.

      Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mouse VEGF (50.0 ng/mL), a concentration of 1.0-2.0 µg/mL of a previous lot of this antibody was required.

      ELISA: A concentration of at least 0.5 µg/mL (100 μL/well) is required to detect 0.2-0.4 ng/well of recombinant mouse VEGF using a previous lot of this antibody.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenHighly pure (>98%) recombinant mouse Vascular Endothelial Growth Factor.
      SpecificityRecognizes Vascular Endothelial Growth Factor (VEGF), a secreted homodimeric protein secreted by a variety of vascularized tissues. The reported activities of VEGF include stimulation of endothelial cell growth, angiogenesis, and capillary permeability.
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG,leading to additional isoforms. [provided by RefSeq].
      Gene Symbol
      • VEGFA
      • VPF
      • VEGF-A
      • VEGF
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis, and induces permeabilization of blood vessels. Binds to the VEGFR1/Flt-1 and VEGFR2/Kdr receptors, heparan sulfate and heparin. Neuropilin-1 binds isoforms VEGF-165 and VEGF-145.
      SUBUNIT: Homodimer; disulfide-linked. Also found as heterodimer with PlGF (By similarity).
      SUBCELLULAR LOCATION: Secreted. Note=VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
      ALTERNATIVE PRODUCTS: 10 named isoforms [FASTA] produced by alternative splicing.
      Molecular Weight27 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on Huvec lysates.

      Western Blot Analysis:
      1:500 dilution of this lot detected VEGF on 10 μg of Huvec lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsReconstituted antibody, at a concentration of ≥ 0.2 mg/mL, is stable for at least six weeks at 2-8ºC. Aliquots stored at -20ºC are stable for at least one year from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information


      Anti-VEGF Antibody SDS


      Safety Data Sheet (SDS) 

      Anti-VEGF Antibody Certificates of Analysis

      TitleLot Number
      Anti-VEGF - 21370372137037
      Anti-VEGF - 23839982383998
      Anti-VEGF - 22804752280475
      Anti-VEGF - DAM1621699DAM1621699
      Anti-VEGF - NG1554817NG1554817
      Anti-VEGF - NG1636567NG1636567
      Anti-VEGF - NG1660993NG1660993
      Anti-VEGF - NG1723322NG1723322
      Anti-VEGF - NG1794849NG1794849


      Reference overviewApplicationPub Med ID
      A novel role of IGFBP7 in mouse uterus: regulating uterine receptivity through Th1/Th2 lymphocyte balance and decidualization.
      Liu, ZK; Wang, RC; Han, BC; Yang, Y; Peng, JP
      PloS one  7  e45224  2012

      Show Abstract
      Western Blotting23028860 23028860
      Prevention of vasa vasorum neovascularization attenuates early neointima formation in experimental hypercholesterolemia.
      Gössl, M; Herrmann, J; Tang, H; Versari, D; Galili, O; Mannheim, D; Rajkumar, SV; Lerman, LO; Lerman, A
      Basic research in cardiology  104  695-706  2009

      Show Abstract Full Text Article
      19458984 19458984

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      Life Science Research > Antibodies and Assays > Primary Antibodies