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MABT134 Anti-VE-cadherin Antibody, clone BV6

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MABT134
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICC, FC, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT134
      ReplacesMAB1989
      DescriptionAnti-VE-cadherin Antibody, clone BV6
      Alternate Names
      • Cadherin-5
      • 7B4 Antigen
      Background InformationHuman Vascular Endothelial (VE)-cadherin is a calcium-dependent adhesion molecule strictly located at cell-to-cell junctions. VE-cadherin is present in all types of endothelium (veins, arteries, capillary and large vessels). Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells. Cadherins may thus contribute to the sorting of heterogeneous cell types. VE-cadherin may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. VE-cadherin also associates with alpha-catenin forming a link to the cytoskeleton.
      References
      Product Information
      FormatPurified
      Control
      • HUVEC cell lysate
      PresentationPurified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-VE-cadherin Antibody, clone BV6 is validated for use in WB, IC, FC, IH(P) for the detection of VE-cadherin.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Flow Cytometry
      • Immunohistochemistry (Paraffin)
      Application NotesImmunocytochemistry Analysis: A 1:500 dilution from a representative lot detected VE-cadherin in the cell-cell junctions of HUVEC cells.

      Immunohistochemistry Analysis: A 1:10-1:20 dilution of a representative lot was used by an independent laboratory in paraffin-embedded tissue sections. Buffered formalin fixation is recommended with a fixation period of no longer than 12 hours. High heat antigen retrieval in citrate buffer (Cat. No. 21545) is also suggested. Antibody can also be used to label acetone-fixed cryostat sections or cells using a immunoperoxidase staining protocol (eg. IHC Select® Detection Kit, Cat. No. DAB150)

      Flow Cytometry Analysis: A representative lot was used in the presence of Ca2+. Note: Use PBS + 2-5mM EDTA for cell detachment.

      Western Blot Analysis: Non-reducing conditions may be needed, Ca2+ required in buffer (2-5 mM).
      Biological Information
      ImmunogenHUVEC cells
      CloneBV6
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is a classical cadherin from the cadherin superfamily and is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. An alternative splice variant has been described but its full length sequence has not been determined.
      Gene Symbol
      • CDH5
      • CD144
      • 7B4
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. Acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction.

      SUBUNIT STRUCTURE: Interacts via cadherin 5 domain with PTPRB. Interacts with TRPC4. Interacts with KRIT1.

      SUBCELLULAR LOCATION: Cell junction. Cell membrane; Single-pass type I membrane protein. Note: Found at cell-cell boundaries and probably at cell-matrix boundaries. KRIT1 and CDH5 reciprocally regulate their localization to endothelial cell-cell junctions.
      Molecular Weight~120 kDa observed.
      The calculated molecular weight is 82 kDa but it will run between ~90-140 kDa because this protein is glycosolated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HUVEC cell lysate.

      Western Blot Anlaysis: A 1:2,000 dilution of this antibody detected VE-cadherin in HUVEC cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-VE-cadherin Antibody, clone BV6 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-VE-cadherin Antibody, clone BV6 Certificates of Analysis

      TitleLot Number
      Anti-VE-cadherin, clone BV6 3082485
      Anti-VE-cadherin, clone BV6 - 2165870 2165870
      Anti-VE-cadherin, clone BV6 - 2383614 2383614
      Anti-VE-cadherin, clone BV6 - 1999459 1999459
      Anti-VE-cadherin, clone BV6 - 2007341 2007341
      Anti-VE-cadherin, clone BV6 - 2061579 2061579
      Anti-VE-cadherin, clone BV6 - 2225193 2225193
      Anti-VE-cadherin, clone BV6 - 2276378 2276378
      Anti-VE-cadherin, clone BV6 - 2309351 2309351
      Anti-VE-cadherin, clone BV6 - 3275081 3275081

      References

      Reference overviewPub Med ID
      ARF6 inhibition stabilizes the vasculature and enhances survival during endotoxic shock.
      Davis, CT; Zhu, W; Gibson, CC; Bowman-Kirigin, JA; Sorensen, L; Ling, J; Sun, H; Navankasattusas, S; Li, DY
      Journal of immunology (Baltimore, Md. : 1950)  192  6045-52  2014

      Show Abstract
      24835390 24835390
      Origin of endothelial progenitors in human postnatal bone marrow.
      Reyes, M; Dudek, A; Jahagirdar, B; Koodie, L; Marker, PH; Verfaillie, CM
      The Journal of clinical investigation  109  337-46  2002

      Show Abstract
      11827993 11827993
      CD34- blood-derived human endothelial cell progenitors.
      Harraz, M; Jiao, C; Hanlon, HD; Hartley, RS; Schatteman, GC
      Stem cells (Dayton, Ohio)  19  304-12  2001

      Show Abstract
      11463950 11463950
      Functional roles for PECAM-1 (CD31) and VE-cadherin (CD144) in tube assembly and lumen formation in three-dimensional collagen gels.
      Yang, S; Graham, J; Kahn, JW; Schwartz, EA; Gerritsen, ME
      The American journal of pathology  155  887-95  1999

      Show Abstract
      10487846 10487846
      Cleavage of beta-catenin and plakoglobin and shedding of VE-cadherin during endothelial apoptosis: evidence for a role for caspases and metalloproteinases.
      Herren, B; Levkau, B; Raines, EW; Ross, R
      Molecular biology of the cell  9  1589-601  1998

      Show Abstract
      9614196 9614196

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      Life Science Research > Antibodies and Assays > Primary Antibodies