IHCR1005-6 Anti-Tyrosine Hydroxylase Antibody, prediluted, clone LNC1

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IHCR1005-6
6 mL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Ch, H, M, Mk, R, Fg, Lz, Vo, Zebrafish IH(P) M Purified Monoclonal Antibody
      Description
      Catalogue NumberIHCR1005-6
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Tyrosine Hydroxylase Antibody, prediluted, clone LNC1
      Background InformationTyrosine hydroxylase plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing.
      References
      Product Information
      FormatPurified
      PresentationLiquid diluted in PBS, pH 7.2 with stabilizers, 0.2% Tween 20, and 0.1% Kathon as preservative.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Tyrosine Hydroxylase Antibody, prediluted, clone LNC1 detects level of Tyrosine Hydroxylase & has been published & validated for use in IH(P).
      Key Applications
      • Immunohistochemistry (Paraffin)
      Application NotesAntibody is prediluted and ready to use for Immunohistochemistry of formalin-fixed, paraffin-embedded tissues.

      Pretreatment: Heat Induced Epitope Retrieval (HIER). Recommend Citrate Buffer, pH 6.0 (Cat. No. 21545). No signal was detected without Epitope retrieval.

      Incubation: 30 minutes with IHC Select® Detection Kits.

      Tyrosine Hydroxylase has been prediluted for use as the primary antibody with Chemicon's IHC Select® Detection Kits and Protocols (Catalog Nos. DAB050, DET-HP1000, APR050, and DET-APR1000), but other supplier's IHC detection systems may be used. For optimized protocol details, visit www.chemicon.com and select the protocols link under Cat. No.IHCR1005-6.
      Biological Information
      ImmunogenTyrosine Hydroxylase purified from PC12 cells.
      EpitopeRecognizes an epitope on the outside of the regulatory N-terminus of tyrosine hydroxylase.
      CloneLNC1
      HostMouse
      SpecificityTyrosine Hydroxylase. Recognizes a protein of approximately 59-63 kDa by Western blot. Does not react with the following on Western Blots: dopamine-beta-hydroxylase, phenylalanine hydroxylase, trytophan hydroxylase, dehydropteridine reductase, sepiapterin reductase, phenethanolamine-N-methyl transferase (PNMT).
      IsotypeIgG1κ
      Species Reactivity
      • Chicken
      • Human
      • Mouse
      • Monkey
      • Rat
      • Frog
      • Lizard
      • Vole
      • Zebrafish
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryTyrosine hydroxylase is involved in the conversion of tyrosine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons.
      Gene Symbol
      • TH
      • TYH
      • EC 1.14.16.2
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P07101 # Plays an important role in the physiology of adrenergic neurons.
      COFACTOR: Fe(2+) ion.
      SIZE: 528 amino acids; 58524 Da
      TISSUE SPECIFICITY: Mainly expressed in the brain and adrenal glands.
      DISEASE: SwissProt: P07101 # Defects in TH are the cause of autosomal recessive Segawa syndrome [MIM:605407]; also known as DOPA-responsive dystonia. Typically, it begins in childhood or adolescence with progressive difficulty in walking and, in some cases, spasticity. Some cases present with parkinsonian symptoms in infancy and are referred to as autosomal recessive infantile parkinsonism.
      SIMILARITY: SwissProt: P07101 ## Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C. Refer to vial for expiration dating.
      Packaging Information
      Material Size6 mL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Tyrosine Hydroxylase Antibody, prediluted, clone LNC1 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Tyrosine Hydroxylase Antibody, prediluted, clone LNC1 Certificates of Analysis

      TitleLot Number
      CHEMICON IHC SELECT RESEARCH -27393562739356
      CHEMICON IHC SELECT RESEARCH PREDILUTED MOUSE ANTI-TYROSINE HYDROXYLASE -25268372526837
      CHEMICON IHC SELECT RESEARCH#210; PREDILUTED MOUSE ANTI-TYROSINE HYDROXYLASE MONOCLONAL ANTIBODY - 20065252006525
      CHEMICON IHC SELECT RESEARCH#210; PREDILUTED MOUSE ANTI-TYROSINE HYDROXYLASE MONOCLONAL ANTIBODY - 20499032049903
      CHEMICON IHC SELECT RESEARCH#210; PREDILUTED MOUSE ANTI-TYROSINE HYDROXYLASE MONOCLONAL ANTIBODY - 22337352233735
      CHEMICON IHC SELECT RESEARCH® PREDILUTED MOUSE ANTI-TYROSINE HYDROXYLASE MONOCLONAL ANTIBODY3049363
      CHEMICON IHC SELECT RESEARCH®PREDILUTED MOUSE ANTI-TYROSINE HYDROXYLASE MONOCLONAL ANTIBODY2925708
      CHEMICON IHC SELECT RESEARCH -26371392637139
      CHEMICON IHC SELECT RESEARCH PREDILUTED MOUSE ANTI-TYROSINE HYDROXYLASE MONOCLONAL ANTIBODY3079079

      References

      Reference overviewApplicationPub Med ID
      Sulpiride, but not SCH23390, modifies cocaine-induced conditioned place preference and expression of tyrosine hydroxylase and elongation factor 1α in zebrafish.
      Darland, T; Mauch, JT; Meier, EM; Hagan, SJ; Dowling, JE; Darland, DC
      Pharmacology, biochemistry, and behavior  103  157-67  2012

      Show Abstract
      Western Blotting22910534 22910534

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