AB5935 Anti-Tyrosine Hydroxylase Antibody, phosphoSer 40

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AB5935
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, M, H, Av ICC, IHC, WB Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue NumberAB5935
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Tyrosine Hydroxylase Antibody, phosphoSer 40
      Alternate Names
      • Tyrosine Monooxygenase
      Background InformationTyrosine hydroxylase plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing.
      References
      Product Information
      FormatAffinity Purified
      Control
      • POSITIVE CONTROL: brain (sympathetic nerve terminals) and adrenal glands.
      PresentationPurified rabbit polyclonal liquid in buffer containing 10mM Hepes, pH 7.5, 150mM NaCl, 100 µg/mL BSA, and 50% glycerol.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Tyrosine Hydroxylase Antibody, phosphoSer 40 detects level of Tyrosine Hydroxylase & has been published & validated for use in IC, IH & WB.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Western Blotting
      Application NotesImmunohistochemistry:
      A 1:1,000 dilution of a previous lot was used in IH.

      Immunocytochemistry:
      A 1:1,000 dilution of a previous lot was used in IC.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenSynthetic peptide from the phosphoSer40 of rat Tyrosine Hydroxylase.
      Epitopephospho-Ser40
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityTyrosine Hydroxylase, phosphoSer40. The antibody recognizes a protein of 60 kDa corresponding to TH phosphorylated at Ser40. Depending on brain region being studied, protein loads and detection methods used some higher molecular weight bands may be detected.
      Species Reactivity
      • Rat
      • Mouse
      • Human
      • Avian
      Species Reactivity NoteRat and mouse. It is expected that the antibody will also react with human, porcine, quail and non-human primates due to sequence homology (100%). Other species have not been tested.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryTyrosine hydroxylase is involved in the conversion of tyrosine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons.
      Gene Symbol
      • TH
      • TYH
      • EC 1.14.16.2
      Modifications
      • Phosphorylation
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P07101 # Plays an important role in the physiology of adrenergic neurons.
      COFACTOR: Fe(2+) ion.
      SIZE: 528 amino acids; 58524 Da
      TISSUE SPECIFICITY: Mainly expressed in the brain and adrenal glands.
      DISEASE: SwissProt: P07101 # Defects in TH are the cause of autosomal recessive Segawa syndrome [MIM:605407]; also known as DOPA-responsive dystonia. Typically, it begins in childhood or adolescence with progressive difficulty in walking and, in some cases, spasticity. Some cases present with parkinsonian symptoms in infancy and are referred to as autosomal recessive infantile parkinsonism.
      SIMILARITY: SwissProt: P07101 ## Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
      Molecular Weight60 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on PC12 lysates.

      Western Blot Analysis:
      1:500 dilution of this lot detected TYROSINE HYDROX PSER40 on 10 μg of PC12 lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 6 months at -20°C in undiluted from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Tyrosine Hydroxylase Antibody, phosphoSer 40 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Tyrosine Hydroxylase Antibody, phosphoSer 40 Certificates of Analysis

      TitleLot Number
      Anti-Tyrosine Hydroxylase, -28042182804218
      Anti-Tyrosine Hydroxylase, phosphoSer 402474866
      Anti-Tyrosine Hydroxylase, phosphoSer 403075635
      Anti-Tyrosine Hydroxylase, phosphoSer 40 - 24303272430327
      Anti-Tyrosine Hydroxylase, phosphoSer 40 - 19744611974461
      Anti-Tyrosine Hydroxylase, phosphoSer 40 - 20740302074030
      Anti-Tyrosine Hydroxylase, phosphoSer 40 - 22816342281634
      Anti-Tyrosine Hydroxylase, phosphoSer 40 - 32097743209774
      Anti-Tyrosine Hydroxylase, phosphoSer 40 - 32885333288533
      Anti-Tyrosine Hydroxylase, phosphoSer 40 - 33890523389052

      References

      Reference overviewApplicationPub Med ID
      Unique Effects of Acute Aripiprazole Treatment on the Dopamine D2 Receptor Downstream cAMP-PKA and Akt-GSK3β Signalling Pathways in Rats.
      Pan, B; Chen, J; Lian, J; Huang, XF; Deng, C
      PloS one  10  e0132722  2015

      Show Abstract
      26162083 26162083
      Bardoxolone Methyl Prevents Fat Deposition and Inflammation in Brown Adipose Tissue and Enhances Sympathetic Activity in Mice Fed a High-Fat Diet.
      Dinh, CH; Szabo, A; Yu, Y; Camer, D; Zhang, Q; Wang, H; Huang, XF
      Nutrients  7  4705-23  2015

      Show Abstract
      26066016 26066016
      Phosphodiesterase 4 inhibition enhances the dopamine D1 receptor/PKA/DARPP-32 signaling cascade in frontal cortex.
      Mahomi Kuroiwa,Gretchen L Snyder,Takahide Shuto,Atsuo Fukuda,Yuchio Yanagawa,David R Benavides,Angus C Nairn,James A Bibb,Paul Greengard,Akinori Nishi
      Psychopharmacology  219  2012

      Show Abstract
      21833500 21833500
      Restricted role of CRF1 receptor for the activity of brainstem catecholaminergic neurons in the negative state of morphine withdrawal.
      Juan-Antonio Garcia-Carmona,Pilar Almela,Alberto Baroja-Mazo,M Victoria Milanes,M Luisa Laorden
      Psychopharmacology  220  2012

      Show Abstract
      21947312 21947312
      Role of corticotropin-releasing factor (CRF) receptor-1 on the catecholaminergic response to morphine withdrawal in the nucleus accumbens (NAc).
      Almela, P; Navarro-Zaragoza, J; García-Carmona, JA; Mora, L; Hidalgo, J; Milanés, MV; Laorden, ML
      PloS one  7  e47089  2012

      Show Abstract
      Western Blotting23071721 23071721
      Genetic deletion of trace amine 1 receptors reveals their role in auto-inhibiting the actions of ecstasy (MDMA).
      Di Cara, B; Maggio, R; Aloisi, G; Rivet, JM; Lundius, EG; Yoshitake, T; Svenningsson, P; Brocco, M; Gobert, A; De Groote, L; Cistarelli, L; Veiga, S; De Montrion, C; Rodriguez, M; Galizzi, JP; Lockhart, BP; Cogé, F; Boutin, JA; Vayer, P; Verdouw, PM; Groenink, L; Millan, MJ
      The Journal of neuroscience : the official journal of the Society for Neuroscience  31  16928-40  2011

      Show Abstract
      Western Blotting22114263 22114263
      Serine 129 phosphorylation reduces the ability of alpha-synuclein to regulate tyrosine hydroxylase and protein phosphatase 2A in vitro and in vivo.
      Lou, H; Montoya, SE; Alerte, TN; Wang, J; Wu, J; Peng, X; Hong, CS; Friedrich, EE; Mader, SA; Pedersen, CJ; Marcus, BS; McCormack, AL; Di Monte, DA; Daubner, SC; Perez, RG
      The Journal of biological chemistry  285  17648-61  2010

      Show Abstract Full Text Article
      20356833 20356833
      14-3-3zeta contributes to tyrosine hydroxylase activity in MN9D cells: localization of dopamine regulatory proteins to mitochondria.
      Wang, J; Lou, H; Pedersen, CJ; Smith, AD; Perez, RG
      The Journal of biological chemistry  284  14011-9  2009

      Show Abstract Full Text Article
      19289463 19289463
      Distinct roles of PDE4 and PDE10A in the regulation of cAMP/PKA signaling in the striatum.
      Nishi, A; Kuroiwa, M; Miller, DB; O'Callaghan, JP; Bateup, HS; Shuto, T; Sotogaku, N; Fukuda, T; Heintz, N; Greengard, P; Snyder, GL
      The Journal of neuroscience : the official journal of the Society for Neuroscience  28  10460-71  2008

      Show Abstract Full Text Article
      18923023 18923023
      Regulation of serine (Ser)-31 and Ser40 tyrosine hydroxylase phosphorylation during morphine withdrawal in the hypothalamic paraventricular nucleus and nucleus tractus solitarius-A2 cell group: role of ERK1/2.
      Cristina Núñez, M Luisa Laorden, M Victoria Milanés
      Endocrinology  148  5780-93  2007

      Show Abstract
      17823252 17823252

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