AB9983 Anti-Tyrosine Hydroxylase Antibody

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AB9983
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, R IH(P), WB Rb Purified Polyclonal Antibody
      Description
      Catalogue NumberAB9983
      DescriptionAnti-Tyrosine Hydroxylase Antibody
      Alternate Names
      • Tyrosine 3-hydroxylase
      • tyrosine 3-monooxygenase
      • tyrosine hydroxylase
      Background InformationTyrosine Hydroxylase plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing.
      References
      Product Information
      FormatPurified
      Control
      • Rat Brain lysate
      PresentationPurified in 0.1M Tris-Glycine (pH7.4) 150mM NaCl with 0.05% NaN3
      Quality LevelMQ100
      Applications
      ApplicationAnti-Tyrosine Hydroxylase Antibody detects level of Tyrosine Hydroxylase & has been published & validated for use in IH(P) & WB.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry: 1:100 working dilutions were used on rat and mouse brain (paraffin embedded tissue).
      Biological Information
      ImmunogenRecombinant protein
      EpitopeC-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityCat. # AB9983 recognizes the C-terminus of Tyrosine Hydroxylase
      Species Reactivity
      • Mouse
      • Rat
      Species Reactivity NoteMouse and Rat. Reactivity with other species has not been determined.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq]
      Gene Symbol
      • TYH
      Purification MethodAntigen Affinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: Plays an important role in the physiology of adrenergic neurons.
      CATALYTIC ACTIVITY: L-tyrosine + tetrahydrobiopterin + O2 = 3,4-dihydroxy-L-phenylalanine + 4a-hydroxytetrahydrobiopterin.
      COFACTOR: Fe2+ ion.
      ENZYME REGULATION: Phosphorylation leads to an increase in the catalytic activity.
      PATHWAY: Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2.
      TISSUE SPECIFICITY: Mainly expressed in the brain and adrenal glands.
      INVOLVEMENT IN DISEASE: Defects in TH are the cause of autosomal recessive Segawa syndrome [MIM:605407]; also known as DOPA-responsive dystonia. Typically, it begins in childhood or adolescence with progressive difficulty in walking and, in some cases, spasticity. Some cases present with parkinsonian symptoms in infancy and are referred to as autosomal recessive infantile parkinsonism.
      SEQUENCE SIMILARITIES: Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
      Molecular Weight~ 58 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8ºC from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Tyrosine Hydroxylase Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Tyrosine Hydroxylase Antibody Certificates of Analysis

      TitleLot Number
      Anti-Tyrosine Hydroxylase - 24595082459508
      Anti-Tyrosine Hydroxylase - 20022042002204
      Anti-Tyrosine Hydroxylase - 20632052063205
      Anti-Tyrosine Hydroxylase - 25332182533218
      Anti-Tyrosine Hydroxylase - 33792673379267
      Anti-Tyrosine Hydroxylase - NG1598673NG1598673
      Anti-Tyrosine Hydroxylase - NG1663847NG1663847
      Anti-Tyrosine Hydroxylase - NG1804944NG1804944
      Anti-Tyrosine Hydroxylase - NG1924569NG1924569
      Anti-Tyrosine Hydroxylase -26084432608443

      References

      Reference overviewPub Med ID
      Unique Effects of Acute Aripiprazole Treatment on the Dopamine D2 Receptor Downstream cAMP-PKA and Akt-GSK3β Signalling Pathways in Rats.
      Pan, B; Chen, J; Lian, J; Huang, XF; Deng, C
      PloS one  10  e0132722  2015

      Show Abstract
      26162083 26162083
      Bardoxolone Methyl Prevents Fat Deposition and Inflammation in Brown Adipose Tissue and Enhances Sympathetic Activity in Mice Fed a High-Fat Diet.
      Dinh, CH; Szabo, A; Yu, Y; Camer, D; Zhang, Q; Wang, H; Huang, XF
      Nutrients  7  4705-23  2015

      Show Abstract
      26066016 26066016

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies