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AB152 Anti-Tyrosine Hydroxylase Antibody

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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, M, Fe, Ft, Sqd, Dr, MlELISA, IF, IHC, IH(P), IP, WBRbAffinity PurifiedPolyclonal Antibody
      Catalogue NumberAB152
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Tyrosine Hydroxylase Antibody
      Alternate Names
      • Tyrosine 3-hydroxylase
      • Tyrosine 3-monooxygenase
      • tyrosine hydroxylase
      • TH
      Background InformationTyrosine hydroxylase (also know as Tyrosine 3-hydroxylase and Tyrosine 3-monooxygenase) plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing.
      Product Information
      FormatAffinity Purified
      • POSITIVE CONTROL: Brain (corpus striatum, sympathetic nerve terminals) and adrenal glands.
      PresentationPurified rabbit polyclonal in buffer containing liquid in 10 mM HEPES (pH 7.5), 150 mM NaCl (pH 7.5), 100 ug/mL BSA and 50% glycerol.
      Quality LevelMQ100
      ApplicationAnti-Tyrosine Hydroxylase Antibody detects level of TH and has been published and validated for use in ELISA, IF, IH, IH(P), IP and WB.
      Key Applications
      • ELISA
      • Immunofluorescence
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunohistochemistry:
      A previous lot of this antibody was uesd at 1:100-1:1,000 dilution, 4°C, for 1-3 days. Stains both fresh frozen and paraffin embedded tissue samples by indirect immunofluorescence and immunoperoxidase.

      A previous lot of this antibody was used at 1:100-1:1,000 dilution.

      A previous lot of this antibody was used on Immunoprecipitation.

      A previous lot of this antibody was used in ELISA.

      Optimal dilutions must be determined by the end user.
      Biological Information
      ImmunogenDenatured tyrosine hydroxylase from rat pheochromocytoma (denatured by sodium dodecyl sulfate).
      SpecificityTyrosine hydroxylase. By western blot, AB152 selective labels a single band at approximately 62kDa (reduced) corresponding to Tyrosine Hydroxylase. It is expected that the antibody will react with most mammalian and many non-mammalian species. It has been reported that this antibody does not work on paraffin embedded human tissue.
      Species Reactivity
      • Human
      • Rat
      • Mouse
      • Feline
      • Ferret
      • Squid
      • Drosophila
      • Mollusk
      Species Reactivity NoteRat, mouse, ferret, feline, Aplysia (marine mollusc). It is expected that the antibody will react with most mammalian and many non-mammalian species. It has been reported that this antibody does not work on paraffin embedded human tissue.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryTyrosine hydroxylase is involved in the conversion of tyrosine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons.
      Gene Symbol
      • TH
      • TYH
      • EC
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P07101 # Plays an important role in the physiology of adrenergic neurons.
      COFACTOR: Fe(2+) ion.
      SIZE: 528 amino acids; 58524 Da
      TISSUE SPECIFICITY: Mainly expressed in the brain and adrenal glands.
      DISEASE: SwissProt: P07101 # Defects in TH are the cause of autosomal recessive Segawa syndrome [MIM:605407]; also known as DOPA-responsive dystonia. Typically, it begins in childhood or adolescence with progressive difficulty in walking and, in some cases, spasticity. Some cases present with parkinsonian symptoms in infancy and are referred to as autosomal recessive infantile parkinsonism.
      SIMILARITY: SwissProt: P07101 ## Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
      Molecular Weight62 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on PC12 lysates.

      Western Blot Analysis:
      1:1000 dilution of this lot detected tyrosine hydroxylase on 10 μg of PC12 lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain frozen at -20ºC to -70ºC in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self defrosting freezer.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information


      Anti-Tyrosine Hydroxylase Antibody SDS


      Safety Data Sheet (SDS) 

      Anti-Tyrosine Hydroxylase Antibody Certificates of Analysis

      TitleLot Number
      Anti-Tyrosine Hydroxylase 3072361
      Anti-Tyrosine Hydroxylase - 2115918 2115918
      Anti-Tyrosine Hydroxylase - 2388829 2388829
      Anti-Tyrosine Hydroxylase - 2392225 2392225
      Anti-Tyrosine Hydroxylase - 2426518 2426518
      Anti-Tyrosine Hydroxylase - 2458991 2458991
      Anti-Tyrosine Hydroxylase - 1951919 1951919
      Anti-Tyrosine Hydroxylase - 1994651 1994651
      Anti-Tyrosine Hydroxylase - 2021194 2021194
      Anti-Tyrosine Hydroxylase - 2038257 2038257


      Reference overviewApplicationSpeciesPub Med ID
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      Van Kampen, JM; Baranowski, DC; Robertson, HA; Shaw, CA; Kay, DG
      PloS one  10  e0139694  2015

      Show Abstract
      26439489 26439489
      Prenatal exposure to methylphenidate affects the dopamine system and the reactivity to natural reward in adulthood in rats.
      Lepelletier, FX; Tauber, C; Nicolas, C; Solinas, M; Castelnau, P; Belzung, C; Emond, P; Cortese, S; Faraone, SV; Chalon, S; Galineau, L
      The international journal of neuropsychopharmacology / official scientific journal of the Collegium Internationale Neuropsychopharmacologicum (CINP)  18  2015

      Show Abstract
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      Sexual dimorphism in the hypophysiotropic tyrosine hydroxylase-positive neurons in the preoptic area of the teleost, Clarias batrachus.
      Saha, S; Patil, S; Singh, U; Singh, O; Singru, PS
      Biology of sex differences  6  23  2015

      Show Abstract
      26557978 26557978
      Catecholamine metabolism in paraganglioma and pheochromocytoma: similar tumors in different sites?
      Grouzmann, E; Tschopp, O; Triponez, F; Matter, M; Bilz, S; Brändle, M; Drechser, T; Sigrist, S; Zulewski, H; Henzen, C; Fischli, S; Abid, K
      PloS one  10  e0125426  2015

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      Neurite Mistargeting and Inverse Order of Intraretinal Vascular Plexus Formation Precede Subretinal Vascularization in Vldlr Mutant Mice.
      Johnson, V; Xiang, M; Chen, Z; Junge, HJ
      PloS one  10  e0132013  2015

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      A method for combining RNAscope in situ hybridization with immunohistochemistry in thick free-floating brain sections and primary neuronal cultures.
      Grabinski, TM; Kneynsberg, A; Manfredsson, FP; Kanaan, NM
      PloS one  10  e0120120  2015

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      Immunohistochemistry25794171 25794171
      Tfap2a and 2b act downstream of Ptf1a to promote amacrine cell differentiation during retinogenesis.
      Jin, K; Jiang, H; Xiao, D; Zou, M; Zhu, J; Xiang, M
      Molecular brain  8  28  2015

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      NMDA receptor GluN2A/GluN2B subunit ratio as synaptic trait of levodopa-induced dyskinesias: from experimental models to patients.
      Mellone, M; Stanic, J; Hernandez, LF; Iglesias, E; Zianni, E; Longhi, A; Prigent, A; Picconi, B; Calabresi, P; Hirsch, EC; Obeso, JA; Di Luca, M; Gardoni, F
      Frontiers in cellular neuroscience  9  245  2015

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      The temporary and accumulated effects of transcranial direct current stimulation for the treatment of advanced Parkinson's disease monkeys.
      Li, H; Lei, X; Yan, T; Li, H; Huang, B; Li, L; Xu, L; Liu, L; Chen, N; Lü, L; Ma, Y; Xu, L; Li, J; Wang, Z; Zhang, B; Hu, X
      Scientific reports  5  12178  2015

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      26220760 26220760
      Analysing human neural stem cell ontogeny by consecutive isolation of Notch active neural progenitors.
      Edri, R; Yaffe, Y; Ziller, MJ; Mutukula, N; Volkman, R; David, E; Jacob-Hirsch, J; Malcov, H; Levy, C; Rechavi, G; Gat-Viks, I; Meissner, A; Elkabetz, Y
      Nature communications  6  6500  2015

      Show Abstract
      25799239 25799239


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