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05-1339 Anti-Trimethyl Histone H3 (Lys4) Antibody, clone CMA304

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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Vrt ELISA, ICC, IP, WB, Mplex, DB M Purified Monoclonal Antibody
      Catalogue Number05-1339
      DescriptionAnti-Trimethyl Histone H3 (Lys4) Antibody, clone CMA304
      Alternate Names
      • H3K4me3
      • Histone H3 (tri methyl K4)
      • H3 histone family, member T
      • histone 3
      • H3
      • histone cluster 3
      • H3
      Background InformationHistones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome. Histones are modified post-translationally; and these modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. The modifications occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle. Trimethylation of histone H3 (Lys4) is is found at the 5' end of active genes and contributes to transcriptional activation by recruiting chromatin-remodelling enzymes. Accordingly, the inactive X chromosome is largely devoid of this mark.
      Product Information
      • HeLa Acid extract lysate
      PresentationPurified mouse monoclonal IgG1κ in PBS with 0.05% sodium azide.
      Quality LevelMQ100
      ApplicationAnti-Trimethyl Histone H3 (Lys4) Antibody, clone CMA304 is a mouse monoclonal antibody for detection of Trimethyl Histone H3 (Lys4) also known as H3K4me3, Histone H3 (tri methyl K4) & has been validated in ELISA, ICC, IP, WB, Mplex, DB.
      Key Applications
      • ELISA
      • Immunocytochemistry
      • Immunoprecipitation
      • Western Blotting
      • Multiplexing
      • Dot Blot
      Application NotesELISA:
      This antibody has been shown by an outside laboratory to be suitable for ELISA.1

      This antibody has been shown by an outside laboratory to be suitable for immunocytochemistry.1

      This antibody has been shown by an outside laboratory to be suitable for immunoprecipitation.1

      This antibody specifically recognizes histone H3 trimethylated on Lys4 by Luminex® assay.
      Biological Information
      ImmunogenSynthetic peptide corresponding to amino acids 1-12 of human Histone H3, trimethylated on Lys4, conjugated to KLH.
      EpitopeTrimethylated Lys4
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody specifically recognizes Histone H3 trimethylated at Lys4. The antibody binding specificity allows for modifications of Lys91.
      Species Reactivity
      • Human
      • Vertebrates
      Species Reactivity NoteHuman. Broad species cross-reactivity is expected, based on sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
      Gene Symbol
      • H3.4
      • H3/g
      • H3/t
      • H3FT
      • H3T
      • H3t
      • MGC126886
      • MGC126888
      • OTTHUMP00000037945
      • Methylation
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

      SUBUNIT STRUCTURE:The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.


      TISSUE SPECIFICITY: Expressed in testicular cells.

      Developmental stage Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.

      PTM:Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 By similarity.

      Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription By similarity.

      Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression By similarity.

      Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin By similarity.

      Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation By similarity.

      Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin.

      Ubiquitinated By similarity.

      SIMILARITY: Belongs to the histone H3 family.
      Molecular Weightapprox. 17 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceWestern Blot Analysis:
      0.5 - 2 μg/mL (1:500-2000) dilution of this antibody detected trimethyl Histone H3 (Lys4) on 10 μg of HeLa acid extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information


      Anti-Trimethyl Histone H3 (Lys4) Antibody, clone CMA304 SDS


      Safety Data Sheet (SDS) 

      Anti-Trimethyl Histone H3 (Lys4) Antibody, clone CMA304 Certificates of Analysis

      TitleLot Number
      Anti-trimethyl Histone H3 (Lys4), clone CMA304 - 21471222147122
      Anti-trimethyl Histone H3 (Lys4), clone CMA304 - 20248972024897
      Anti-trimethyl Histone H3 (Lys4), clone CMA304 - NG1683902NG1683902
      Anti-trimethyl Histone H3 (Lys4), clone CMA304 - NG1726948NG1726948
      Anti-trimethyl Histone H3 (Lys4), clone CMA304 - NG1809717NG1809717
      Anti-trimethyl Histone H3 (Lys4), clone CMA304 - NG1897032NG1897032
      Anti-trimethyl Histone H3 (Lys4), clone CMA304 - NRG1587636NRG1587636
      Anti-trimethyl-Histone H3 (Lys4), clone -25697862569786
      Anti-trimethyl-Histone H3 (Lys4), clone -26587492658749
      Anti-trimethyl-Histone H3 (Lys4), clone -26974052697405


      Reference overviewApplicationPub Med ID
      Embryonic MicroRNA-369 Controls Metabolic Splicing Factors and Urges Cellular Reprograming.
      Konno, M; Koseki, J; Kawamoto, K; Nishida, N; Matsui, H; Dewi, DL; Ozaki, M; Noguchi, Y; Mimori, K; Gotoh, N; Tanuma, N; Shima, H; Doki, Y; Mori, M; Ishii, H
      PloS one  10  e0132789  2015

      Show Abstract
      26176628 26176628
      Decreased histone H2B monoubiquitination in malignant gastric carcinoma.
      Wang, ZJ; Yang, JL; Wang, YP; Lou, JY; Chen, J; Liu, C; Guo, LD
      World journal of gastroenterology  19  8099-107  2013

      Show Abstract
      24307806 24307806
      AHT-ChIP-seq: a completely automated robotic protocol for high-throughput chromatin immunoprecipitation.
      Aldridge, S; Watt, S; Quail, MA; Rayner, T; Lukk, M; Bimson, MF; Gaffney, D; Odom, DT
      Genome biology  14  R124  2013

      Show Abstract
      24200198 24200198
      Id2 complexes with the SNAG domain of Snai1 inhibiting Snai1-mediated repression of integrin β4.
      Chang, C; Yang, X; Pursell, B; Mercurio, AM
      Molecular and cellular biology  33  3795-804  2013

      Show Abstract
      23878399 23878399
      Mutations in EDM2 selectively affect silencing states of transposons and induce plant developmental plasticity.
      Tsuchiya, T; Eulgem, T
      Scientific reports  3  1701  2013

      Show Abstract
      23609044 23609044
      An alternative polyadenylation mechanism coopted to the Arabidopsis RPP7 gene through intronic retrotransposon domestication.
      Tsuchiya, T; Eulgem, T
      Proceedings of the National Academy of Sciences of the United States of America  110  E3535-43  2013

      Show Abstract
      23940361 23940361
      Transcriptional activation of transposable elements in mouse zygotes is independent of Tet3-mediated 5-methylcytosine oxidation.
      Inoue, A; Matoba, S; Zhang, Y
      Cell research  22  1640-9  2012

      Show Abstract
      23184059 23184059
      Regulation of DCC localization by HTZ-1/H2A.Z and DPY-30 does not correlate with H3K4 methylation levels.
      Petty, E; Laughlin, E; Csankovszki, G
      PloS one  6  e25973  2011

      Show Abstract
      Immunofluorescence21998734 21998734
      Global mapping of H3K4me3 and H3K27me3 reveals specificity and plasticity in lineage fate determination of differentiating CD4+ T cells.
      Wei, G; Wei, L; Zhu, J; Zang, C; Hu-Li, J; Yao, Z; Cui, K; Kanno, Y; Roh, TY; Watford, WT; Schones, DE; Peng, W; Sun, HW; Paul, WE; O'Shea, JJ; Zhao, K
      Immunity  30  155-67  2009

      Show Abstract
      19144320 19144320
      The organization of histone H3 modifications as revealed by a panel of specific monoclonal antibodies.
      Kimura, Hiroshi, et al.
      Cell Struct. Funct., 33: 61-73 (2008)  2008

      18227620 18227620

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