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05-584 Anti-Tie2/TEK Antibody, clone Ab33

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05-584
200 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, PoWB, IP, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-584
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Tie2/TEK Antibody, clone Ab33
      Alternate Names
      • CD202b antigen
      • TEK tyrosine kinase, endothelial
      • Tunica interna endothelial cell kinase
      • Tyrosine-protein kinase receptor TEK
      • Tyrosine-protein kinase receptor TIE-2
      • p140 TEK
      • soluble TIE2 variant 1
      • soluble TIE2 variant 2, venous malformations, multiple cutaneous and mucosal
      Background InformationTIE2 (tyrosine kinase with Ig and EGF homology domains 2) is expressed almost exclusively in endothelial cells in mice, rats and humans. This receptor possesses a unique extracellular domain containing two immunoglobulin-like loops separated by three epidermal growth factor-like repeats that are connected to three fibronectin type III-like repeats. The ligand for the receptor is Angiopoietin 1. Defects in TIE2 are associated with inherited venous malformations; the TIE2 signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis.
      References
      Product Information
      FormatPurified
      Control
      • HUVEC cell lysate
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide before the addition of glycerol to 30%. Liquid at -20°C.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Tie2/TEK Antibody, clone Ab33 is validated for use in WB, IP, IH for the detection of Tie2/TEK.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunohistochemistry
      Application NotesImmunoprecipitation: This antibody immunoprecipitated Tie2/TEK, as reported by an independent laboratory.

      Immunohistochemistry: Reported to detect Tie2/TEK in frozen, Triton-treated sections.
      This antibody is not suitable for paraffin-embedded sections.
      Biological Information
      ImmunogenFusion protein corresponding to residues 1-745 of human Tie2/TEK with a C-terminal His6-tag purified from infected SF-9 cells. Clone Ab33
      Cloneclone Ab33
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes Tie2/TEK, Mr 145kDa
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Pig
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe TEK receptor tyrosine kinase is expressed almost exclusively in endothelial cells in mice, rats, and humans. This receptor possesses a unique extracellular domain containing 2 immunoglobulin-like loops separated by 3 epidermal growth factor-like repeats that are connected to 3 fibronectin type III-like repeats. The ligand for the receptor is angiopoietin-1. Defects in TEK are associated with inherited venous malformations; the TEK signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis. TEK is closely related to the TIE receptor tyrosine kinase.
      Gene Symbol
      • TEK
      • CD202B
      • TIE2
      • VMCM1
      • VMCM
      • hTIE2
      • CD202b
      • TIE-2
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q02763 # This protein is a protein tyrosine-kinase transmembrane receptor for angiopoietin 1. It may constitute the earliest mammalian endothelial cell lineage marker. Probably regulates endothelial cell proliferation, differentiation and guides the proper patterning of endothelial cells during blood vessel formation.
      SIZE: 1124 amino acids; 125811 Da
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      TISSUE SPECIFICITY: Predominantly expressed in endothelial cells and their progenitors, the angioblasts. Has been directly found in placenta and lung, with a lower level in umbilical vein endothelial cells, brain and kidney.
      DISEASE:SwissProt: Q02763 # Defects in TEK are a cause of dominantly inherited venous malformations (VMCM) [MIM:600195]; an error of vascular morphogenesis characterized by dilated, serpiginous channels.
      SIMILARITY: SwissProt: Q02763 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. Tie subfamily. & Contains 3 EGF-like domains. & Contains 3 fibronectin type-III domains. & Contains 2 Ig-like C2-type (immunoglobulin-like) domains. & Contains 1 protein kinase domain.
      Molecular Weight145 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by western blot on RIPA lysates from HUVEC cells.

      Western Blot Analysis: 0.2-1 μg/mL of this antibody detected Tie2/TEK in RIPA lysates from HUVEC cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at from date of receipt.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Tie2/TEK Antibody, clone Ab33 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Tie2/TEK Antibody, clone Ab33 Certificates of Analysis

      TitleLot Number
      Anti-SAP, clone SAP23.1.5 - DAM1407394 DAM1407394
      Anti-Tie2/TEK, clone Ab33 3050684
      Anti-Tie2/TEK, clone Ab33 - 20177 20177
      Anti-Tie2/TEK, clone Ab33 - 21771 21771
      Anti-Tie2/TEK, clone Ab33 - 23758 23758
      Anti-Tie2/TEK, clone Ab33 - 25989 25989
      Anti-Tie2/TEK, clone Ab33 - 26069 26069
      Anti-Tie2/TEK, clone Ab33 - 3173792 3173792
      Anti-Tie2/TEK, clone Ab33 - 3218956 3218956
      Anti-Tie2/TEK, clone Ab33 - 3244041 3244041

      References

      Reference overviewApplicationPub Med ID
      Delivery of therapeutic siRNA to the lung endothelium via novel Lipoplex formulation DACC.
      Fehring, V; Schaeper, U; Ahrens, K; Santel, A; Keil, O; Eisermann, M; Giese, K; Kaufmann, J
      Molecular therapy : the journal of the American Society of Gene Therapy  22  811-20  2014

      Show Abstract
      Western Blotting24390281 24390281
      Activation of hypoxia response in endothelial cells contributes to ischemic cardioprotection.
      Kerkelä, R; Karsikas, S; Szabo, Z; Serpi, R; Magga, J; Gao, E; Alitalo, K; Anisimov, A; Sormunen, R; Pietilä, I; Vainio, L; Koch, WJ; Kivirikko, KI; Myllyharju, J; Koivunen, P
      Molecular and cellular biology  33  3321-9  2013

      Show Abstract
      Western Blotting23775121 23775121
      The flow dependency of Tie2 expression in endotoxemia.
      Kurniati, Neng F, et al.
      Intensive Care Med, (2013)  2013

      Show Abstract
      23563632 23563632
      Autocrine role of angiopoietins during megakaryocytic differentiation.
      Saulle, E; Guerriero, R; Petronelli, A; Coppotelli, E; Gabbianelli, M; Morsilli, O; Spinello, I; Pelosi, E; Castelli, G; Testa, U; Coppola, S
      PloS one  7  e39796  2012

      Show Abstract
      Western Blotting22792187 22792187
      Increased Expression of Angiopoietins and Tie2 in the Lungs of Chronic Asthmatic Mice.
      Makinde TO, Agrawal DK
      Am J Respir Cell Mol Biol  2010

      Show Abstract
      20463289 20463289
      Angiopoietin-1/Tie-2 activation contributes to vascular survival and tumor growth during VEGF blockade.
      Huang, J; Bae, JO; Tsai, JP; Kadenhe-Chiweshe, A; Papa, J; Lee, A; Zeng, S; Kornfeld, ZN; Ullner, P; Zaghloul, N; Ioffe, E; Nandor, S; Burova, E; Holash, J; Thurston, G; Rudge, J; Yancopoulos, GD; Yamashiro, DJ; Kandel, JJ
      International journal of oncology  34  79-87  2009

      Show Abstract Full Text Article
      19082480 19082480
      Antiangiogenic gene therapy targeting the endothelium-specific receptor tyrosine kinase Tie2.
      Lin, P, et al.
      Proc. Natl. Acad. Sci. U.S.A., 95: 8829-34 (1998)  1998

      Show Abstract
      9671764 9671764
      Expression of Tie2/Tek in breast tumour vasculature provides a new marker for evaluation of tumour angiogenesis.
      Peters, K G, et al.
      Br. J. Cancer, 77: 51-6 (1998)  1998

      Show Abstract
      9459145 9459145
      Tie2 expression and phosphorylation in angiogenic and quiescent adult tissues.
      Wong, A L, et al.
      Circ. Res., 81: 567-74 (1997)  1997

      Show Abstract
      Immunohistochemistry (Tissue)9314838 9314838

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies