AB976 Anti-Thyroid Stimulating Hormone Antibody

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AB976
500 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Ca, H, R, Sh, Fe IH(P) Rb Serum Polyclonal Antibody
      Description
      Catalogue NumberAB976
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Thyroid Stimulating Hormone Antibody
      Alternate Names
      • TSH
      References
      Product Information
      FormatSerum
      PresentationLiquid rabbit antiserum containing 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Thyroid Stimulating Hormone Antibody detects level of Thyroid Stimulating Hormone & has been published & validated for use in IH(P).
      Key Applications
      • Immunohistochemistry (Paraffin)
      Application NotesTISSUE EVALUATION:

      Formalin-fixed and paraffin embedded sections (6 microns thick) of anterior pituitary glands were prepared from a number of species including man, cat, rat, dog, ox and sheep. Cryostat sections (10 microns thick) of the above fixed tissues can also be used in the staining procedures.



      IMMUNOPEROXIDASE

      STAINING:

      1. The sections to be stained are hydrated to phosphated buffered saline (PBS, pH 7.4).

      2. The antiserum diluted 1:1,000 (in PBS) is allowed to react with the tissue at 37º C for 30 min.

      3. Using PBS, the tissue is washed for 15 min at room temperature and treated sequentially with goat anti-rabbit gamma globulin (diluted 1:20 in PBS) and peroxidase anti-peroxidase complex (diluted 1:100 in PBS) for 30 min at 37º C. After each of these steps, the tissue is washed with PBS as before.

      4. The final stage of the reaction is treatment of the section with a mixture of 3,3'-diaminbenzidine HC1 (0.02%) in hydrogen peroxide (0.004%). For preservation, the sections are dehydrated, cleared and mounted.



      IMMUNO-

      FLUORESCENCE

      STAINING: 1. Same as Step 1 above.

      2. The tissue is reacted with the antiserum, diluted 1:20-1:40 in (PBS) for 30 min at 37º C.

      3. After washing off the excess antiserum with PBS, the section is treated fluorescein labeled goat anti-rabbit gamma globulin serum (diluted 1:10 in PBS) for 30 min at 37º C. The tissue is washed with PBS and after mounting under saline-gylcerol, is viewed with a fluorescence microscope.



      COMMENTS: The antiserum described here has been established as giving a positive staining on the thyrotrophs when the above protocol is used. Final working dilutions must be determined by end user. In general, staining can occur on further dilution of the primary antiserum if this is accompanied by an increase in its incubation time, e.g. 24 hours at either 37º C or 4º C.
      Biological Information
      ImmunogenHighly purified TSH.
      HostRabbit
      SpecificityThe antiserum supplied has been evaluated for its ability to give a positive and specific immunostaining of thyrotropin stimulating hormone (TSH)-containing cells using both the immunoperoxidase or immunofluorescence procedures.
      Species Reactivity
      • Canine
      • Human
      • Rat
      • Sheep
      • Feline
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TSHB
      • TSH-BETA
      • TSH-beta
      • TSH-B
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P01222 # Indispensable for the control of thyroid structure and metabolism.
      SIZE: 138 amino acids; 15609 Da
      SUBUNIT: Heterodimer of a common alpha chain and a unique beta chain which confers biological specificity to thyrotropin, lutropin, follitropin and gonadotropin.
      SUBCELLULAR LOCATION: Secreted.
      SIMILARITY: SwissProt: P01222 ## Belongs to the glycoprotein hormones subunit beta family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsLyophilized: 20°C for up to 18 months. Reconstituted: -20° C for up to 12 months. Do not thaw and refreeze reconstituted material.
      Packaging Information
      Material Size500 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Thyroid Stimulating Hormone Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Thyroid Stimulating Hormone Antibody Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-THYROTROPIN STIMULATING HORMONE (TSH) POLYCLONAL ANTIBODY - 24203712420371
      RABBIT ANTI-THYROTROPIN STIMULATING HORMONE (TSH) - 25523582552358
      RABBIT ANTI-THYROTROPIN STIMULATING HORMONE (TSH) -26890922689092
      RABBIT ANTI-THYROTROPIN STIMULATING HORMONE (TSH) -28202642820264
      RABBIT ANTI-THYROTROPIN STIMULATING HORMONE (TSH) POLYCLONAL ANTIBODY - 20069092006909
      RABBIT ANTI-THYROTROPIN STIMULATING HORMONE (TSH) POLYCLONAL ANTIBODY - 20313212031321
      RABBIT ANTI-THYROTROPIN STIMULATING HORMONE (TSH) POLYCLONAL ANTIBODY - 20614192061419
      RABBIT ANTI-THYROTROPIN STIMULATING HORMONE (TSH) POLYCLONAL ANTIBODY - 21910592191059
      RABBIT ANTI-THYROTROPIN STIMULATING HORMONE (TSH) POLYCLONAL ANTIBODY - 22192622219262
      RABBIT ANTI-THYROTROPIN STIMULATING HORMONE (TSH) POLYCLONAL ANTIBODY - 22833112283311

      References

      Reference overviewApplicationPub Med ID
      Insm1 controls development of pituitary endocrine cells and requires a SNAG domain for function and for recruitment of histone-modifying factors.
      Welcker, JE; Hernandez-Miranda, LR; Paul, FE; Jia, S; Ivanov, A; Selbach, M; Birchmeier, C
      Development (Cambridge, England)  140  4947-58  2013

      Show Abstract
      24227653 24227653
      Expression of P2Y receptors in the rat anterior pituitary.
      Yu, Q; Guo, W; Song, X; Liu, X; Xiang, Z; He, C; Burnstock, G
      Purinergic signalling  7  207-19  2011

      Show Abstract Full Text Article
      Immunohistochemistry21567129 21567129
      Rat thyroid hyperplasia induced by gestational and lactational exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin.
      N Nishimura, J Yonemoto, Y Miyabara, M Sato, C Tohyama, N Nishimura, J Yonemoto, Y Miyabara, M Sato, C Tohyama
      Endocrinology  144  2075-83  2003

      Show Abstract
      12697716 12697716
      Immunohistochemical localization of thyroid stimulating hormone induced by a low oral dose of 2,3,7,8-tetrachlorodibenzo-p-dioxin in female Sprague-Dawley rats.
      Noriko Nishimura, Yuichi Miyabara, Mikio Sato, Junzo Yonemoto, Chiharu Tohyama
      Toxicology  171  73-82  2002

      Show Abstract
      11836014 11836014
      Immunohistochemical study of the postnatal development of pituitary thyrotrophs in the rat, with special reference to cluster formation.
      Yashiro, T, et al.
      Cell Tissue Res., 216: 39-46 (1981)  1981

      Show Abstract
      7226208 7226208
      Localization of thyrotropin (TSH) in the dog pituitary gland.
      el-Etreby, M F and el-Bab, M R
      Cell Tissue Res., 186: 399-412 (1978)  1978

      Show Abstract
      75068 75068