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MABN2320-100UG Anti-TREM-2 Antibody, C-terminal Antibody, clone 14D1

MABN2320-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABN2320-100UG
      DescriptionAnti-TREM-2 Antibody, C-terminal Antibody, clone 14D1
      Alternate Names
      • Triggering receptor expressed on myeloid cells 2
      • Triggering receptor expressed on monocytes 2
      Background InformationTriggering receptor expressed on myeloid cells 2 (UniProt: Q99NH8; also known as TREM-2, Triggering receptor expressed on monocytes 2) is encoded by the Trem2 (also known as Trem2a, Trem2b, Trem2c) gene (Gene ID: 83433) in murine species. TREM-2 is a single-pass type I monomeric membrane protein that forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells. TREM-2 is expressed at higher levels in the central nervous system (CNS), heart, and lung. At lower levels it is also expressed in lymph nodes and in non-lymphoid tissues such as kidney, liver and testis. In the CNS it is highly expressed in microglia, where it, along with its adaptor protein DAP12, regulates inflammatory cytokine release and phagocytosis of apoptotic neurons. It plays a role in chronic inflammation and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. TREM-2 forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells. Apolipoprotein E (ApoE) is reported to function as a high-affinity ligand for TREM-2. ApoE binding is shown to enhance the phagocytosis of apoptotic N2a cells by primary microglia cells. Greatly reduced ApoE binding is seen with Alzheimer s disease-associated TREM2 R47H mutant. TREM2 is synthesized with a signal peptide (aa 1-18) that is cleaved off to generate the mature form with a large extracellular region (aa 19-171) that contains the V-type Ig-like domain, a single-transmembrane domain (aa 172-192), and a cytoplasmic tail (aa 193-227). The short cytoplasmic tail does not contain any signaling motifs and relies on its association with the immunoreceptor tyrosine-based activation motif- (ITAM-) containing TYROBP for signal transduction. (Ref.: Kleinberger, G., et al. (2017). EMBO J. 36(13); 1837-1853).
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Trem2, C-terminal, clone 14D1, Cat. No. MABN2320, is a highly specific rat monoclonal antibody, that targets Triggering receptor expressed on myeloid cells 2 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry (Paraffin) Analysis: A 1:25 dilution from a representative lot detected TREM-2 in mouse cerebral cortex and olfactory lobe tissue sections.

      Western Blotting Analysis: A representative lot detected TREM-2 in Western Blotting applications (Kleinberger, G., et. al. (2017). EMBO J. 36(13):1837-1853).
      Biological Information
      ImmunogenA linear peptide corresponding to 20 amino acids from the C-terminal region of murine Triggering receptor expressed on myeloid cells 2 (TREM-2).
      Clone14D1
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityClone 14D1 is a rat monoclonal antibody that specifically detects murine Triggering receptor expressed on myeloid cells 2 (TREM-2). It targets an epitope with in 20 amino acids from the C-terminal region.
      IsotypeIgG2aκ
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Trem2
      • Trem2a
      • Trem2c
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight24.53 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in mouse hindbrain tissue sections.

      Immunohistochemistry (Paraffin) Analysis: A 1:25 dilution of this antibody detected TREM-2 in mouse hindbrain tissue sections.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-TREM-2 Antibody, C-terminal Antibody, clone 14D1 Certificates of Analysis

      TitleLot Number
      Anti-TREM-2, C-terminal, clone 14D1 - 35027353502735
      Anti-TREM-2, C-terminal, clone 14D1 - Q3224764Q3224764

      References

      Reference overviewPub Med ID
      The FTD-like syndrome causing TREM2 T66M mutation impairs microglia function, brain perfusion, and glucose metabolism
      Gernot Kleinberger 1 2 , Matthias Brendel 3 , Eva Mracsko 4 , Benedikt Wefers 5 6 , Linda Groeneweg 4 , Xianyuan Xiang 1 , Carola Focke 3 , Maximilian Deußing 3 , Marc Suárez-Calvet 1 5 , Fargol Mazaheri 5 , Samira Parhizkar 1 , Nadine Pettkus 1 , Wolfgang Wurst 2 5 6 7 , Regina Feederle 2 5 8 , Peter Bartenstein 2 3 , Thomas Mueggler 4 , Thomas Arzberger 5 9 10 , Irene Knuesel 4 , Axel Rominger 2 3 , Christian Haass
      EMBO J  36(13)  1837-1853  2017

      Show Abstract
      28559417 28559417