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ST1704 Anti-TOMM22 Mouse mAb (4G4)

Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H, M, R M Monoclonal Antibody

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ST1704-100UG
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      100 μg
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      Description
      OverviewRecognizes the ~18 kDa TOMM22 protein in PC-12, NIH3T3, Raw 264.7, and HeLa cells and human small intestine tissue.
      Catalogue NumberST1704
      Brand Family Calbiochem®
      SynonymsAnti-Translocase of Outer Mitochondrial Membrane 22
      Application Data
      Detection of rat TOMM22 by immunoblotting. Sample: Whole cell lysate from PC-12 cells. Primary antibody: Anti-TOMM22 Mouse mAb (4G4) (Cat. No. ST1704) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of mouse TOMM22 by immunoblotting. Sample: Whole cell lysate from Raw 264.7 cells. Primary antibody: Anti-TOMM22 Mouse mAb (4G4) (Cat. No. ST1704) (1-5 µg/ml). Detection: chemilimunescence.

      Detection of human TOMM22 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-TOMM22 Mouse mAb (4G4) (Cat. No. ST1704) (10 µg/ml). Detection: fluorescence.

      Detection of human TOMM22 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human small intestine tissue. Primary antibody: Anti-TOMM22 Mouse mAb (4G4) (Cat. No. ST1704) (3 µg/ml). Detection: colorimetric (DAB).
      References
      Product Information
      Formliquid
      FormulationIn PBS, pH 7.2.
      Negative control293T cells
      Positive controlPC 12 cells, NIH3T3 cells, Raw 264.7 cells, HeLa cells, Human small intestine tissue
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Paraffin Sections
      Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Application NotesELISA (see coments)
      Immunoblotting (1-5 µg/ml)
      Immunocytochemistry (10 µg/ml)
      Paraffin Sections (3 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenFull-length, recombinant, human TOMM22 (aa 1-143), expressed as a GST fusion protein
      Clone4G4
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-TOMM22 Mouse mAb (4G4) SDS

      Title

      Safety Data Sheet (SDS) 

      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision10-November-2010 RFH
      SynonymsAnti-Translocase of Outer Mitochondrial Membrane 22
      ApplicationELISA (see coments)
      Immunoblotting (1-5 µg/ml)
      Immunocytochemistry (10 µg/ml)
      Paraffin Sections (3 µg/ml)
      Application Data
      Detection of rat TOMM22 by immunoblotting. Sample: Whole cell lysate from PC-12 cells. Primary antibody: Anti-TOMM22 Mouse mAb (4G4) (Cat. No. ST1704) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of mouse TOMM22 by immunoblotting. Sample: Whole cell lysate from Raw 264.7 cells. Primary antibody: Anti-TOMM22 Mouse mAb (4G4) (Cat. No. ST1704) (1-5 µg/ml). Detection: chemilimunescence.

      Detection of human TOMM22 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-TOMM22 Mouse mAb (4G4) (Cat. No. ST1704) (10 µg/ml). Detection: fluorescence.

      Detection of human TOMM22 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human small intestine tissue. Primary antibody: Anti-TOMM22 Mouse mAb (4G4) (Cat. No. ST1704) (3 µg/ml). Detection: colorimetric (DAB).
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~18 kDa TOMM22 protein.
      BackgroundThe protein encoded by this gene is an integral membrane protein of the mitochondrial outer membrane. The encoded protein interacts with TOMM20 and TOMM40, and forms a complex with several other proteins to import cytosolic preproteins into the mitochondrion.
      HostMouse
      ImmunogenFull-length, recombinant, human TOMM22 (aa 1-143), expressed as a GST fusion protein
      Clone4G4
      IsotypeIgG2a
      Specieshuman, mouse, rat
      Positive controlPC 12 cells, NIH3T3 cells, Raw 264.7 cells, HeLa cells, Human small intestine tissue
      Negative control293T cells
      Formliquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review